PREPARATION AND PROPERTIES OF MICRO ENZYME SENSORS FOR CHOLESTEROL AND CHOLESTEROL ESTER

胆固醇和胆固醇酯微酶传感器的制备及性能

• 批准号: 05640686
• 负责人: MOTONAKA Junko
• 金额: $ 1.28万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640686/
• 关键词: Cholesterol; cholesterol ester; Micro-enzyme sensors

Micro-enzyme sensors using cholesterol oxidase (EC 1.1.3.6) and cholesterol esterase (EC 3.1.1.13) were developed for measuring cholesterol and cholesterol ester. The platinum micro-sensors (platinum diameter, 50 mum) were etched in hot aqua regia to create a cavity at their tip. A porous composite material prepared from acetylene black and Teflon emulsion was packed into this cavity and the redox mediator [Os (bpy)_3] (PF_6)_2 was monitored by cyclic voltammetry in the potential range of 200 - 900 mV.The micro-sensors were dipped overnight in buffer solution containing the desired enzyme to immobilize it on the tip by adsorption. Calibration curves for measurements of cholesterol and cholesterol ester, the effects of pH,temperature, and concomitant compounds, the life-time of the micro-sensors, and their availability for measuring cholesterol and cholesterol ester in urine were examined. Under optimal conditons, the response of the sensors were linear in concentration ranges of 5 muM - 0.47 mM cholesterol and 2 muM - 1.00 mM cholesterol ester.

研制了胆固醇氧化酶（EC 1.1.3.6）和胆固醇酯酶（EC 3.1.1.13）微酶传感器，用于测定胆固醇和胆固醇酯。铂微传感器（铂直径，50 μ m）在热王水中蚀刻以在其尖端处产生腔。将乙炔黑和聚四氟乙烯乳液制成的多孔复合材料填充到该空腔中，在200 - 900 mV的电位范围内用循环伏安法监测氧化还原介体[Os（bpy）_3]（PF_6）_2。测定胆固醇和胆固醇酯的校准曲线，pH值，温度和伴随化合物的影响，微传感器的寿命，以及它们用于测量尿液中胆固醇和胆固醇酯的可用性进行了检查。在最佳条件下，胆固醇和胆固醇酯的浓度分别在5 μ M- 0.47 mM和2 μ M- 1.00 mM范围内呈线性关系。

项目成果

Junko Motonaka: "Determination of cholesterol and cholesterol Ester with Novel Enzyme microsensors" Analytical Chemistry. 65. 3258-3261 (1993)

Junko Motonaka：“用新型酶微传感器测定胆固醇和胆固醇酯”分析化学。

Junko Motonaka: "Detemination of cholesterol and cholesterol Ester with Novel Enzyme Microsensors" Analytical Chemistry. 65. 3258-3261 (1993)

Junko Motonaka：“用新型酶微传感器测定胆固醇和胆固醇酯”分析化学。

Junko Motonaka: "Determination of cholesterol and cholesterol Esteriwith Novel qngyme microsensors" Analytical Chemistry. 65. 3258-3261 (1993)

Junko Motonaka：“用新型 qngyme 微传感器测定胆固醇和胆固醇酯”分析化学。

Junko Motonaka: "Determination of Cholesterol and Cholesterol Ester with Novel Enzyme Microsensors" Analygtical chemistry. 65. 3258-3261 (1993)

Junko Motonaka：“用新型酶微传感器测定胆固醇和胆固醇酯”分析化学。