Preparation of the oligo-DNA modified electrode and application of recognition for DNA segment

寡聚DNA修饰电极的制备及其在DNA片段识别中的应用

• 批准号: 13640607
• 负责人: MOTONAKA Junko
• 金额: $ 2.3万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640607/
• 关键词: DNA modified electrode; recognition for DNA segment; Osmium complex; mediator; センサ試作

A novel immobilized DNA electrode based on a porous carbon electrode was prepared to study interactions between DNA and chemical compounds. The characteristics of this electrode were evaluated by electrochemical methods, and then the interaction between DNA and an osmium complex was studied. On this electrode (electrode surface ; 0.66 - 2.37 cm^2), DNA was immobilized at 0.26 x 10^<-4>-1.23 x 10^<-4> mol cm^<-2>. Hybridization of DNA was observed with a relative standard deviation of 11% (n=4). The tris(bipyridyl)osmium(II/III) ion (osmium complex) was found to interact to major or minor grooves. Using this osmium complex, a DNA sequence could be recognized with more than 12 mer of the same sequence.It was found that the immobilization using only physical adsorption gave some limitation in the analysis because of the relatively large variation of immobilization. Improvement of immobilization treatment, which is in progress, would enable these electrodes to be applied for detailed study of various interactions.

制备了一种新型的基于多孔碳电极的DNA固定化电极，用于研究DNA与化合物的相互作用。用电化学方法对该电极的性能进行了评价，并研究了其与DNA的相互作用。在该电极上(电极表面0.66~2.37 cm^2)，DNA在0.26×10~(-4)-1.23×10~(-4)mol·cm~(-4)~(-2)处被固定。DNA杂交的相对标准偏差为11%(n=4)。研究发现，三(联吡啶基)Os(II/III)离子与主槽或次槽相互作用。结果发现，由于固定化的变化量较大，仅用物理吸附的固定化方法在分析中存在一定的局限性。正在进行的固定化处理的改进将使这些电极能够应用于各种相互作用的详细研究。

项目成果

Junko Motonaka: "Electro chemical and DNA-binding properties of dipyridophenazine complexes of osmium (II)"Journal of Electroanalytical Chemistry. 510. 96-102 (2001)

Junko Motonaka：“锇 (II) 联吡啶吩嗪复合物的电化学和 DNA 结合特性”电分析化学杂志。

Junko Motonaka: "E valuation of kinetic parameter of bipyrizine type osmium complex for enzyme reaction"Analytical Science. 2001. 1367-1370 (2002)

Junko Motonaka：“酶反应联吡嗪型锇络合物动力学参数的E评估”分析科学。

Junko Motonaka: "Evaluation of kinetic parameter of bipyrizine type osmium complexes for enzyme reaction"Analytical Sciences. 2001. 1367-1370 (2002)

Junko Motonaka：“酶反应联吡嗪型锇复合物动力学参数的评估”分析科学。

Junko Motonaka: "Electrochemical and DNA-bending properties dipyridophenazine complexes of osmium(11)"Journal of Electroanalytical Chemistry. 510. 96-102 (2001)

Junko Motonaka：“锇联吡啶吩嗪复合物的电化学和 DNA 弯曲特性 (11)”电分析化学杂志。

Junko Motonaka: "Electrochemical and DNA-binding properties of dipyridophenazine complexes of osmium (II)"Journal of Electroanalytical Chemistry. 510. 96-102 (2001)

Junko Motonaka：“锇 (II) 联吡啶吩嗪复合物的电化学和 DNA 结合特性”电分析化学杂志。