MICRO-ENZYME SENSORS WITH OSMIUMN COMPLEX AS MEDIATOR FOR L-AMINO ACIDS AND D-AMINO ACIDS

以锇复合​​物作为 L-氨基酸和 D-氨基酸介体的微酶传感器

• 批准号: 07640810
• 负责人: MOTONAKA Junko
• 金额: $ 1.47万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640810/
• 关键词: L-amino acid; D-amino acid; Micro-enzyme sensors; optical isomers; 光学異性体

Micro-enzyme sensors using L-amino-acid oxidase (EC 1.4.3.2) and D-amino-acid oxidase (EC 1.4.3.3) were developed for measuring L- and D-amino acids. The platinum micro-sensors (platinum diameter, 30 mum) were etched in hot aqua regia to create a cavity at their tip. A porous composite material prepared from acetylene black and Teflon emulsion was packed into the tip of the platinum micro-sensors and the redox mediator [Os (bpy)_3 ] (PF_6)_2 was monitored by cyclic voltammetry in the potential range 200-900 mV.The micro-sensors were dipped overnight in buffer solution containing the desired enzyme to immobilize in on the tip by adsorption method. Calibration curves for measurements of L- and D-amino acids, the effects of pH,temperature, and concomitant compounds, the life-time of the micro-sensors, and their availability for measuring L-amino acids in urine were examined. Under optimal conditions, the response of sensors was linear in the concentration ranges of 3.3-240mg/l L-amino acids and 0.83-17mg/l D-amino acids.

用L-氨基酸氧化酶(EC 1.4.3.2)和D-氨基酸氧化酶(EC 1.4.3.3)制作了测定L和D-氨基酸的微酶传感器。铂金微传感器(铂直径，30微米)在热的王水中蚀刻，在其尖端形成一个空腔。将乙炔黑和聚四氟乙烯乳液制成的多孔复合材料填充到铂微传感器的尖端，用循环伏安法在200-900 mV范围内监测氧化还原介体[Os(Bpy)_3](PF_6)_2。将微传感器浸泡在含有所需酶的缓冲液中过夜，用吸附法将其固定在传感器的尖端。考察了L氨基酸和D-氨基酸测定的校正曲线、pH、温度和伴生化合物的影响、微传感器的寿命及其对尿液中L氨基酸测定的有效性。在最佳条件下，传感器的线性范围分别为：L氨基酸3.3~240 mg/L，D-氨基酸0.83~17 mg/L。

项目成果

Junko Motonaka: "Micro-enzyme sensors with osmium complex as mediator for L- and D- amino acids" Journal of Electro analytical chemistry. 373. 75-81 (1994)

Junko Motonaka：“以锇复合物作为 L- 和 D- 氨基酸介体的微酶传感器”《电分析化学杂志》。

Junko Motonaka: "Preparation and properties of micro-enzyme sensors for L-amino acid and D-amino acid" Chemical Sensers I1. 83-7. 763-770 (1993)

Junko Motonaka：“L-氨基酸和D-氨基酸微酶传感器的制备和性能”化学传感器I1。

Junko Motonaka: "Preparation and prepartes of micro enziyrce sensers for L-amino acid and D-amino acid" chemical Sensors II. 83-7. 763-770 (1993)

Junko Motonaka：“L-氨基酸和D-氨基酸微酶传感器的制备和制备”化学传感器II。

Junko Motonaka: "Preparation and properties of micro enzyme sensors for L-amino acid and D-amino acid" Chemical Sensors II, (M.Butler, A.Ricco and N.Yamazoe, eds), The Electrochemical Society, Inc., New Jersey, U.S.A.Vol.83-7. 763-770 (1993)

Junko Motonaka：“L-氨基酸和 D-氨基酸微酶传感器的制备和特性”化学传感器 II（M.Butler、A.Ricco 和 N.Yamazoe，编辑），电化学协会，Inc.，新

Junko Motonaka: "Micro-engyme sensors with osmium complex as mediator for L- and D- amino acids" Journal of Electro analytical chemistry. 373. 75-81 (1994)

Junko Motonaka：“以锇络合物作为 L- 和 D- 氨基酸介体的微酶传感器”《电分析化学杂志》。