Molecular evolutionary studies on the origin of eukaryotes-Analyzes of informational macromolecules.

真核生物起源的分子进化研究-信息大分子的分析。

• 批准号: 05640793
• 负责人: INOUE Tadashi
• 金额: $ 1.28万
• 依托单位: The Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640793/
• 关键词: Nosema bombycis; Ribosomal RNA; Elongation factor; elongation factor; 微胞子虫; rRNA遺伝子; 分子進化; 真核生物

In order to investigate the evolutionary origin of eukaryotes, the sequences of ribosomal RNA genes and elongation factors of some microsporidia including Nosema bombycis which is believed to diverge at early stage of eukaryotes are examined by conventional methods.The originisms used were two strains of N.bombycis, Vairimorpha sp. M12 and Pleistophora sp.. The target genes were PCR-amplified by using appropriate primers from the total genomic DNA which had been extracted from their spores. The complete sequences of the small subunit ribosomal RNA (SSU rRNA) genes were obtained from the above four samples. The lengths of the SSU rRNAs were shorter than those of ordinary prokaryotes, and the secondary structure models constructed from the sequence lacked several stems which are common for the eukaryotic SSU rRNAs. This fact may indicate that the microsporidian SSU rRNA stayd in the primitive evolutional stage.Attempt to sequence the elongation factors was failed because of the lack of information for the appropriate primers to amplify the gene.

为了探讨真核生物的进化起源，本文以家蚕微孢子虫（Nosema bombycis）的两个菌株-变形虫（Vairimorphasp.M12）和丛孢霉（Pleistophora sp.M12）为出发菌株，用常规方法对家蚕微孢子虫（Nosema bombycis）等被认为在真核生物早期分化的微孢子虫的核糖体RNA基因和延伸因子的序列进行了分析。从其孢子中提取总基因组DNA，用合适的引物对目的基因进行PCR扩增。从上述四个样品中获得了小亚基核糖体RNA（SSU rRNA）基因的完整序列。SSU rRNA的长度比普通原核生物短，并且由该序列构建的二级结构模型缺少真核SSU rRNA常见的几个茎。这一事实可能表明微孢子虫SSU rRNA仍处于原始进化阶段。由于缺乏合适的引物来扩增该基因的信息，尝试对延伸因子进行测序失败。

项目成果

Kawakami, Y., Inoue, T., Uchida, Y., Hatakeyama, Y., Iwano, H.and Ishihara, R.: "Specific amplification of DNA from reference starains of Nosema bombycis (Nosematidae, Microsporidia) ." J.seric.Sci.Jpn.64 (in press). (1995)

Kawakami, Y.、Inoue, T.、Uchida, Y.、Hatakeyama, Y.、Iwano, H. 和 Ishihara, R.：“来自家蚕微孢子虫（微孢子虫科、微孢子虫）参考 Starains 的 DNA 的特异性扩增。”

Kawakami,Y.,Inoue,T.,Uchida,Y.,Hatakeyama,Y.,Iwano,H.and Ishinara,R.: "Specific amplification of DNA from reference strains of Nosema bombycis(Nosematidae,Microsporidia)." J.Seric.Sci.Jpn.64(in press). (1995)

Kawakami,Y.、Inoue,T.、Uchida,Y.、Hatakeyama,Y.、Iwano,H. 和 Ishinara,R.：“家蚕微孢子虫（微孢子虫科、微孢子虫）参考菌株 DNA 的特异性扩增。”

Kawakami,Y.,Inoue,T.,Uchida,Y.,Hatakeyama,Y.,Iwano,H.and Ishihara,R.: "Specific amplification of DNA from reference strains of Nosema bombycis(Nosematidae,Microsporidia)." J.Seric.Sci.Jpn.64(in press). (1995)

Kawakami,Y.、Inoue,T.、Uchida,Y.、Hatakeyama,Y.、Iwano,H. 和 Ishihara,R.：“来自家蚕微孢子虫（Nosematidae、微孢子虫）参考菌株 DNA 的特异性扩增。”