Glycoalkaloids as the resistant factor for potato disease.

配糖生物碱作为马铃薯病害的抗性因子。

• 批准号: 05660077
• 负责人: GOTO Kazushige
• 金额: $ 0.83万
• 依托单位: SASEBO NATIONAL COLLEGE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660077/
• 关键词: Potato scab; alpha-solanine; alpha-chaconine; Glycoalkaloid; グリコアルカロイド; 耐病性要因; alpha-ソラニン; alpha-チャコニン; 緑化処理

The purpose of this study is to use the metabolic intermediate in leaf and stem for early diagnosis of soil borne disease. Glycoalkaloids contents in potato were investigated for the indicator of potato scab infection.1.The change of Glycoalkaloids contents in potato tubers, in case of greening by sun light, that treatment was done instead of disease infection, were investigated.The Glycoalkaloids content in potato tubers of disease resistant variety was higher than susceptible variety.Analyzed Glycoalkaloid's components were alpha-Solanine and alpha-Chaconine. The increasing rate of alpha-Chaconine in potato tubers by greening, was higher than alpha-Solanine content.From these results, it is assumed that the step of Glycoalkaloids increasing start from the increase of alpha-Chaconine and then it transfer to alpha-Solanine increase.These increase of Glycoalkaloids in the potato tubers by greening shows that the total Glycoalkaloids have the function of phytoalexins.2.The contents of Glycoalkaloids in leaves of each position on stem were that the three leaves from top were much more Glycoalkaloids contained than lower leaves, these contents shows much Glycoalkaloids accumulate in upper leaves.The content of Glycoalkaloids in leaves of two potato variaties were investegated. The variety of Dejima, it is more susceptible for potato scab, contained more less Glycoalkaloids than none susceptible variety, May Queen.In this study, We could confirmed the positive relation between the contents of Glycoalkaloids and infection susceptibility on potato scab.

本研究的目的是利用叶片和茎中的代谢中间体进行土传疾病的早期诊断。以马铃薯中糖生物碱含量作为马铃薯结痂感染的指标。研究了在日光绿化条件下，不进行病害侵染处理，马铃薯块茎中糖生物碱含量的变化。抗病品种马铃薯块茎中糖生物碱含量高于敏感品种。糖生物碱的主要成分为-龙葵碱和-恰康碱。马铃薯块茎中α -茶碱的增殖率高于α -龙葵碱。从这些结果可以推测，糖生物碱增加的步骤是从-恰康碱增加开始的，然后转移到-龙葵碱增加。马铃薯块茎中总糖生物碱的增加说明总糖生物碱具有抗菌素的作用。茎上各位置叶片的糖生物碱含量均表现为上3个叶片的糖生物碱含量明显高于下3个叶片，说明糖生物碱主要集中在上部叶片。对两个马铃薯品种叶片中糖生物碱的含量进行了研究。Dejima品种对马铃薯结痂更敏感，其糖生物碱含量比不敏感品种少，May Queen。本研究证实了糖生物碱含量与马铃薯赤霉病感染敏感性呈正相关。