Early diagnosis of soil borne disease infection by metabolic intermediate in leaf and stem

本研究的目的是利用叶片和茎中的代谢中间产物进行土传病害的早期诊断。以马铃薯中的糖苷生物碱含量作为马铃薯疮痂病侵染的指标.经分析，糖苷生物碱的主要成分为α-龙葵碱和α-茶乌头碱。研究了马铃薯疮痂病茎段和未发病茎段中糖苷生物碱的含量。茎上各部位叶片中生物碱的含量，从上到下三片叶片中生物碱含量明显高于下部叶片，说明上部叶片中积累较多.对部分马铃薯品种叶片中糖苷生物碱的含量进行了测定。感病品种千羽和立花的糖基生物碱含量明显低于不感病品种五月皇后。叶片和块茎中糖苷生物碱含量的相关性不显著。而茎部叶位和根部块茎中糖苷生物碱含量与α-龙葵碱含量呈正相关.施钙肥对块茎中糖苷生物碱含量的影响表现为施钙肥增加了西丰和五月皇后的块茎中糖苷生物碱含量。这种增加是由于α-查乌头碱含量增加的结果.本研究未能确定糖碱的指示性含量，但证实了糖碱用于马铃薯疮痂病侵染诊断的可能性。

The purpose of this study is to use the metabolic intermediate in leaf and stem for early diagnosis of soil borne disease. Glycoalkaloids contents in potato were investigated for the indicator of potato scab infection.1. Analyzed Glycoalkaloid's components were alpha-Solanine and alpha-Chaconine. The contents of Glycoalkaloids in the potato scab infected stems and none infected stem were investigated. The contents of Glycoalkaloids in leaves of each position on stem were that the three leaves from top were much more Glycoalkaloids contended than lower leaves, these contents shows much Glycoalkaloids accumulate in upper leaves.2. The content of Glycoalkaloids in leaves of some potato varieties were investigated. The variety of Chigiwa and Tachibana, They are more susceptible for potato scab were contented more less Glycoalkaloids than none susceptible variety, May Queen.3. The corelation of Glycoalkaloids contents between in leaf and tuber were no significant. But the correlation between Glycoalkaloids contents of leaf position on stem and potato tubers on root were positively corelated on alpha-solanine content.4. The change of Glycoalkaloids contents in tubers for the application of calcium fertilizer were that the increase of Glycoalkaloids content by calcium application was found at the variety of Nishiyutaka and May Queen. This increases were result of increasing of alpha-chaconine content.5. In this study, We could not decide the indicative contents of Glycoalkaloids, but the possibility of utilization for infection diagnosis on potato scab were confirmed.