An HPLC analysis and telemetric study of crustacean cardiac performance

采用高效液相色谱法对克氏原螯虾（Procambarus clarkii）和美洲龙虾（Homarus americanus）的血淋巴进行了分析。从用冰麻醉30分钟或搅拌10分钟的动物中获得血样。在小龙虾中，麻醉动物和战斗动物分别显示出较低和较高的5-HT水平（5.3 +/- 1.0和17.3 +/- 3.0 pg/10 ul HPLC样品）。我们发现，5-HT的代谢物5-HIAA在小龙虾中也显示出相同的趋势，麻醉动物和战斗动物的水平分别较低和较高（71.1 ± 14.5和29.8 ± 14.1 pg/10 μ l血液HPLC样品）。其他物质在血液中的浓度在麻醉和战斗动物之间没有变化：平均浓度为20 μ g/l去甲肾上腺素，3 μ g/l HVA（多巴胺代谢物），0.01 mg/l GABA，6 mg/l天冬氨酸和12 mg/l谷氨酸。然而，我们发现，麻醉和战斗龙虾显示，分别较低和较高的多巴胺水平，在同一龙虾（n=5动物）进行比较。目前的调查显示，血液中的神经激素显着改变的行为状态，即战斗或休息。

We performed HPLC analysis of hemolymph of a crayfish (Procambarus clarkii) and the American lobster (Homarus americanus). Blood samples were obtained from either animals anesthetized with ice for 30 min or agitated for 10 min.In the crayfish, anesthetized animal and fighting animal showed, respectively, lower and higher 5-HT levels (5.3 +/- 1.0 and 17.3 +/- 3.0 pg/10 ul HPLC sample). We found that 5-HIAA, a metabolite of 5-HT, also shows the same trend in the crayfish, lower and higher levels, respectively, for anesthetized and fighting animals (71.1 +/- 14.5 and 29.8+-14.1 pg/10 ul blood HPLC sample). Other substances in blood were not changed in concentration between anesthetized and fighting animals : mean concentrations were 20 ug/l noradrenaline, 3 ug/l HVA (a dopamine metabolite), 0.01 mg/l GABA, 6 mg/l aspartate, and 12 mg/l glutamate.In the lobster, concentrations of 5-HT and 5-HIAA were found to be lower than the detection limit of HPLC, 0.5 pg/10 ul sample. However, we found that anesthetized and fighting lobster shows, respectively, lower and higher dopamine levels, performing the comparison in the same lobster (n=5 animals).The present investigation revealed that neurohormones in the blood are significantly changed by behavioral state, that is, fighting or resting.