An HPLC analysis and telemetric study of crustacean cardiac performance

甲壳动物心脏性能的 HPLC 分析和遥测研究

• 批准号: 09640811
• 负责人: YAZAWA Toru
• 金额: $ 0.26万
• 依托单位: TOKYO METROPOLITAN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640811/
• 关键词: crustacea; heart; serotonin; dopamine; 5-HIAA; HPLC; noradrenaline; telemetry; 血液; アメリカザリガニ; アメリカンロブスター; 行動; モノアミン; アミノ酸; 神経伝達物質; 威かく行動; ホルモン

We performed HPLC analysis of hemolymph of a crayfish (Procambarus clarkii) and the American lobster (Homarus americanus). Blood samples were obtained from either animals anesthetized with ice for 30 min or agitated for 10 min.In the crayfish, anesthetized animal and fighting animal showed, respectively, lower and higher 5-HT levels (5.3 +/- 1.0 and 17.3 +/- 3.0 pg/10 ul HPLC sample). We found that 5-HIAA, a metabolite of 5-HT, also shows the same trend in the crayfish, lower and higher levels, respectively, for anesthetized and fighting animals (71.1 +/- 14.5 and 29.8+-14.1 pg/10 ul blood HPLC sample). Other substances in blood were not changed in concentration between anesthetized and fighting animals : mean concentrations were 20 ug/l noradrenaline, 3 ug/l HVA (a dopamine metabolite), 0.01 mg/l GABA, 6 mg/l aspartate, and 12 mg/l glutamate.In the lobster, concentrations of 5-HT and 5-HIAA were found to be lower than the detection limit of HPLC, 0.5 pg/10 ul sample. However, we found that anesthetized and fighting lobster shows, respectively, lower and higher dopamine levels, performing the comparison in the same lobster (n=5 animals).The present investigation revealed that neurohormones in the blood are significantly changed by behavioral state, that is, fighting or resting.

本文对克氏原螯虾（Procambarus clarkii）和美洲大龙虾（Homarus americanus）的血淋巴进行了高效液相色谱分析。对冰麻醉30分钟或搅拌10分钟的动物采集血样。在小龙虾、麻醉动物和打斗动物中，5-HT水平分别较低和较高（HPLC样品为5.3 +/- 1.0和17.3 +/- 3.0 pg/10 ul）。我们发现5-羟色胺的代谢物5-HIAA在小龙虾中也表现出同样的趋势，麻醉和战斗动物的5-HIAA分别较低和较高（血液HPLC样品为71.1 +/- 14.5和29.8+-14.1 pg/10 ul）。麻醉动物和搏斗动物血液中其他物质的浓度没有变化：平均浓度为去甲肾上腺素20微克/升，HVA（多巴胺代谢物）3微克/升，GABA 0.01毫克/升，天冬氨酸6毫克/升，谷氨酸12毫克/升。在龙虾中，5-HT和5-HIAA的浓度低于HPLC的检测限0.5 pg/10 ul样品。然而，我们发现麻醉龙虾和战斗龙虾分别显示出较低和较高的多巴胺水平，在同一只龙虾（n=5只动物）中进行比较。目前的研究表明，在行为状态下，即战斗或休息，血液中的神经激素会发生显著的变化。

项目成果

赤野史典: "ラットのPO/AHへの高カリウムRinger液かん流が体温調節に及ぼす影響" 法政大学多摩研究報告. 13. 37-42 (1998)

Fuminori Akano：“高钾林格溶液灌注至大鼠 PO/AH 对体温调节的影响”法政大学多摩研究报告。13. 37-42 (1998)。

T.Yazawa: "A pharmacological and HPLC analysis of the excitatory transmitters of the cardiac ganglion in the heart of the isopod crustacen, Bathynomus doederleini." Can.J.Physiol.Pharmacol.76. 599-604 (1998)

T.Yazawa：“对甲壳等足类动物 Bathynomus doederleini 心脏中的心脏神经节的兴奋性递质进行药理学和 HPLC 分析。”

S.Matsumura: "Serotonergic control of the heart and pericardium in the chiton Acanthopleura japonica" Comp.Biochem.Physiol.A. in press. (1999)

S.Matsumura：“日本棘皮鳖心脏和心包的血清素控制”Comp.Biochem.Physiol.A。

Hiroshi Ando, Toru Yazawa and Kiyoaki Kuwasawa: "Cardioacceleratory reflexes triggered by mechanoproprioceptors of the swimmerets in the stomatopod crustacean Squilla oratoria" Comp.Biochem.Physiol.C.(in press).

Hiroshi Ando、Toru Yazawa 和 Kiyoaki Kuwasawa：“由口足类甲壳动物虾蛄中游泳足的机械本体感受器触发的心脏加速反射”Comp.Biochem.Physiol.C.（出版中）。

M.Yasumatsu, T.: "Monoamines, amino acids snd acetylcholine in the preoptic area and anterior hypothalamus of rats : measurements of tissue extracts and in vivo microdialysates." Comp.Biochem.Physiol.A. 121. 13-23 (1998)

M.Yasumatsu, T.：“大鼠视前区和下丘脑前部的单胺、氨基酸和乙酰胆碱：组织提取物和体内微透析液的测量。”