Construction of physical map chicken W chromosome

鸡W染色体实物图谱构建

• 批准号: 05660333
• 负责人: HASHIGUCHI Tsutomu
• 金额: $ 1.41万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660333/
• 关键词: Chicken; Genome library; W chromosome; Reiterated sequence; DNA fingerprinting; Restriction enzyme cleavage map; W染色体; コンティグマップ; コスミドベクター; ゲノムライブラリー; ドットハイブリダイゼーション; サザンハイブリダイゼーション

Chicken genomic library which was constructed with both of restriction enzyme Sau3A I digest of female chicken genomic DNA and cosmid vector super Cos1 were analyzed with two chicken W chromosome specific reiterated sequences pUGD0601 (a 0.7kb unit of the Xho I family) and pUGD1201 (a 1.2kb unit of the EcoR I family). It was detected the chicken W chromosome specific DNA library with 900 clone of Xho I family and 600 clone EcoR I family, respectively. In the results of dot blot hybridization analysis, the 85% clone had W chromosome specific sequences, and it was suggested that this library covered 72% of the amount of chicken W chromosomal genome. The restriction enzyme cleavage map of each clone were constructed by southern blot hybridization analysis among the cosmid clone and short and long arm of the super Cos 1, and analyzed each clone by using DNA finger printing. These results suggested that the mean of the size of insert was about 40kb, and these library were equivalent to 2.1% of whole W chromosome genome.

利用鸡W染色体特异重复序列pUGD 0601（Xho Ⅰ家族的0.7kb单位）和pUGD 1201（EcoR Ⅰ家族的1.2kb单位），对用Sau3A Ⅰ酶切雌鸡基因组DNA和超粘粒载体super Cos1构建的鸡基因组文库进行分析。对含有900个Xho I家族克隆和600个EcoR I家族克隆的鸡W染色体特异DNA文库进行了检测。斑点杂交结果表明，85%的克隆具有W染色体特异性序列，表明该文库覆盖了鸡W染色体基因组的72%。通过Southern印迹杂交分析，将粘粒克隆与super Cos 1的短臂和长臂进行杂交，构建各克隆的限制性内切酶酶切图谱，并对各克隆进行DNA指纹图谱分析。结果表明，插入片段的平均大小约为40kb，约占W染色体全基因组的2.1%。

项目成果

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H.Yamashita、T.Hashiguchi 等人：“日本本土马 DNA 指纹的群体分化”J.Equine Sci.5：（印刷中）。

H.Yamashita,T.Hashiguchi et al.: "Population Differentiation of Japanese Native Horse DNA Fingerprinting." J.Equine.Sci.5(発表予定). (1995)

H.Yamashita、T.Hashiguchi 等人：“日本本土马 DNA 指纹图谱的群体分化”。J.Equine.Sci.5（即将发表）。

H.Yamashita,T.Hashiguchi et al.: "Genetic Relationships Among Domestic and Jungle Fowls Revealed by DNA Fingerprinting Analysis." Jpn.Poult.Sci.31. 335-344 (1994)

H.Yamashita、T.Hashiguchi 等人：“通过 DNA 指纹分析揭示家禽和原鸡之间的遗传关系”。

H.Yamashita, T.Hashiguchi et al.: "DNA fingerprinting analysis of native and red jungle fowls in Fiji and Western Samoa." Jpn.Poult. Sci.32 : (in press). (1995)

H.Yamashita、T.Hashiguchi 等人：“斐济和西萨摩亚本土原鸡和红原鸡的 DNA 指纹分析。”

H.Yamashita,T.Hashiguchi et al.: "Genetic Fingerprinting in Chicken by Amplification of Polymorphic DNA." Biochem.Genet.33(発表予定). (1995)

H. Yamashita、T. Hashiguchi 等人：“通过多态性 DNA 扩增进行鸡基因指纹分析”。Biochem.Genet.33（即将发表）。