Trans-splicing occurs after cis-splicing and polyadenylation in processing of Onchocerca volvulus actin mRNA

在锥虫和线虫中发现了一种称为反式剪接的新型转录后过程。在这个过程中，一个被称为剪接前导体（SL）的RNA序列被剪接到一个前体mRNA中，以创建成熟mRNA的5'片段。由于线虫既可以进行反式剪接，也可以进行顺式剪接，因此线虫是研究顺式剪接和反式剪接机制的良好模型。本研究旨在寻找含有或不含SL内含子的未成熟mrna，以便研究顺式剪接和反式剪接之间的时间关系。成虫cDNA文库。利用5′非翻译区和编码区两个探针对扭转菌进行筛选。本研究分离到的两个具有编码区的cdna克隆、五个内含子中的一个克隆和不带SL的poly-A尾部克隆表明，在反式剪接和顺式剪接完成之前，聚腺苷化可以发生。顺式剪接也可以发生在反式剪接之前。

A novel post-transcriptional process called trans-splicing has been found in trypanosomes and nematodes. In this process an RNA sequence, called the spliced leader (SL) , is spliced into a precursor mRNA to create the 5' segment of the mature mRNA.Since both trans-splicing and cis-splicing occur in nematodes, nematodes is a good model for investigations of mechanism between cis-and trans-splicing. The present investigation was conducted to seek immature mRNAs possessing some introns with or without the SL so that the temporal relationship between cis-and trans-splicing could be examined. cDNA libraries of adult 0.volvulus were screened with two probes, the 5'untranslated region and the coding region region. The two cDNAs clones having coding region, one of five introns, and poly-A tail without SL which were isolated in the present study indicate that polyadenylation can occur before trans-splicing and before cis-splicing is complete. Cis-splicing can also occur before trans-splicing.