Evaluation of neurotoxic parameter of neurotransimitter release on learning disorder caused by industrial chemicals

工业化学品引起的学习障碍神经递质释放的神经毒性参数评价

• 批准号: 05670367
• 负责人: SHIGETA Sadayoshi
• 金额: $ 0.96万
• 依托单位: Tokai University school
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670367/
• 关键词: Toluene; Carbon-monoxide; Leaning Behavior; Spontaneous Activity; Acethylcholine; DOPAC; HVA; 5-HIAA; 回避学習行動; 神経伝達物質; モノアミン代謝物; 大脳皮質; 線条体

Usefulness as a parameter to evaluate the neurotoxicity on learning disorder caused by toluene and carbon monoxide were studied by shock avoidance results and release of ACh from cerebral cortex and ACh, DOPAC,HVA,5-HIAA from stiatum.Neurotransmitters in cerebral cortex and striatum were measured on the high avoidance rat strain (THA rat) and the low avoidance rat strain (WS rat) for clarify the areas of brain and the kinds of neurotransmitters to control leaning avoidance behavior. ACh release in cerebral cortex and striatum, and monoamine metabolites in striatum were related to avoidance ability between the high and low avoidance rat strains. Change of spontaneous activities of the rats were related to release of ACh and monoamine metabolites on toluene exposure. Release of ACh and DOPAC,HVA,5-HIAA increased when spontaneous activities rose by the exciting action of toluene, and release of neurotransmitters decreased when spontaneous activities suppressed caused by the anesthetic action of toluene. Increase of lever-pressing on shock avoidance behavior seemed to be caused by the exciting action of toluene. The effects of CO exposure on leaning behavior and neurotransmitters were not so clear as toluene exposure.

通过休克避免结果、脑皮质乙酰胆碱（ACh）释放量和脑脊液中乙酰胆碱（ACh）、多巴胺（DOPAC）、HVA、5-HIAA的释放量来评价甲苯和一氧化碳所致学习障碍的神经毒性。对高回避大鼠品系（THA）和低回避大鼠品系（WS）的大脑皮层和纹状体神经递质进行了测定，以明确控制学习回避行为的脑区和神经递质种类。高、低回避大鼠品系的回避能力与大脑皮层和纹状体的乙酰胆碱释放及纹状体单胺代谢有关。大鼠自发活动的变化与接触甲苯后乙酰胆碱和单胺代谢产物的释放有关。甲苯的兴奋作用使自发性活动升高时，ACh、DOPAC、HVA、5-HIAA的释放增加；甲苯的麻醉作用使自发性活动受到抑制时，神经递质释放减少。压杆对避震行为的增加似乎是由甲苯的刺激作用引起的。一氧化碳暴露对学习行为和神经递质的影响不如甲苯暴露那么明显。