Alveolar macrophage-derived chemotactic factor for airway epithelial cells

肺泡巨噬细胞衍生的气道上皮细胞趋化因子

• 批准号: 05670519
• 负责人: SHOJI Shunsuke
• 金额: $ 1.54万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670519/
• 关键词: epithelial cells; chemotaxis; alveolar macrophase; mast cells; fibronectin; neutrophils; ファイブロネクチン; 間質性肺疾患; 肺サルコイドーシス

The title of this project is "alveolar macrophage-derived chemotactic factor for airway epithelial cells".However, the significant chemotactic activity has not been identified in spite of the continuous and many experiments after all. The project has been the branch of our big project titled "the airway epithelial cells and the related cell chemotaxis", so we now report our results from two important research for the mastcell chemotaxis and the neutrophil chemotaxis, both of which are closely related to this project.(1)Bronchial epithelial cells release fibronectin as a chemotactic factor for mast cells.We cultured bovine bronchial epithelial cells and obtained the culture supernatant conditioned medium. The supernatant had significantly more chemotactic activity for the rat basophilic leukemia(RBL-2H3) cells and rat peritoneal mast cells compared with the culture medium. The chemotactic activity was then purified by three serial column chromatographies using HPLC,and finally identified as bovine fibronectin.(submitted)(2)Cigarette smoke stimulates release of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells.Bovine bronchial epithelial cells were cultured and the water-soluble extract of cigarette smoke was added into the culture medium. The culture supermatant conditioned medium were harvested after six hours incubation. The smoke extract contained culture supernatant contained significantly more chemotactic activity for human peripheral blood-derived neutrophils compared with the supernatant without smoke extract. The chemotactic activity appeared to be a lipoxygenase product(s).(published)

本课题的研究题目为“肺泡巨噬细胞源性气道上皮细胞趋化因子”，经过连续多次的实验，至今尚未发现其具有明显的趋化活性。本课题是我们的重大项目“气道上皮细胞及其相关细胞趋化性”的分支，现报告与本课题密切相关的两个重要研究结果：肥大细胞趋化性和中性粒细胞趋化性。（1）支气管上皮细胞释放纤维连接蛋白作为肥大细胞的趋化因子。培养上清对大鼠嗜碱性白血病细胞（RBL-2 H3）和大鼠腹腔肥大细胞的趋化活性明显高于培养上清。然后通过使用HPLC的三个连续柱层析纯化趋化活性，最后鉴定为牛纤维连接蛋白。（2）香烟烟雾刺激培养的牛支气管上皮细胞释放中性粒细胞趋化活性培养牛支气管上皮细胞，并将香烟烟雾的水溶性提取物加入培养基中。培养6小时后收获培养上清液条件培养基。与不含烟雾提取物的培养上清液相比，含有烟雾提取物的培养上清液对人外周血来源的中性粒细胞的趋化活性显著增加。趋化活性似乎是脂氧合酶产物。（已出版）

项目成果

Shoji S,Ertl RF etal: "Cigaretle smoke stimulates relense of neutrophill chem activity from cuured bovine.cells." Clinical Science. 88. 337-344 (1995)

Shoji S、Ertl RF 等人：“香烟烟雾会刺激培养牛细胞中性粒细胞化学活性的减弱。”

Shoji S, Erfl RF, Robbins R, etal: "Cigarette smoke stimulates releve of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells" Clinical Science. 88. 337-344 (1995)

Shoji S、Erfl RF、Robbins R 等人：“香烟烟雾刺激培养的牛支气管上皮细胞中性粒细胞趋化活性的释放”临床科学。

Shoj S,Ertl RF,KoyamaS,Robbins R,LeikaufG,Von Essen S and Rennard SI: "Cigarette smoke stimulates release of neutrophil chemofuctic activity" Clinical Science. 88. 337-344 (1995)

Shoj S、Ertl RF、KoyamaS、Robbins R、LeikaufG、Von Essen S 和 Rennard SI：“香烟烟雾刺激中性粒细胞化学活性的释放”临床科学。