AIRWAY MUCOSA CELLS PRODUCE CHEMOTACTIC FACTOR(S) FOR MAST CELLS.

气道粘膜细胞为肥大细胞产生趋化因子。

• 批准号: 08670657
• 负责人: SHOJI Shunsuke
• 金额: $ 1.34万
• 依托单位: The UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670657/
• 关键词: CHEMOTAXIS; MAST CELLS; AIRWAY MUCOSA; FIBROBLASTS; VASCULAR ENDOTHELIAL CELLS

In the allergic diseases, including bronchial asthma, the airway mast cells in the bronchial mucosa may contribute to the pathogenesis of such diseases by releasing a variety of mediators and cytokines. Although the relation between airway mast cells and the peribronchial fibrosis have recently been highlighted, it is not known which types of cells in the airway mucosa are capable of releasing chemotactic factor(s) for those mast cells. In the present study, we cultured bronchial epithelial cells, fibroblasts and vascular endothelial cells in vitro, and assayed for the chemotactic activity in the culture supernatant conditioned media of those cells. The results demonstrated that bronchial epithelial cells, fibroblasts and vascular endothelial cells can release chemotactic activity for mast cells, and one of the potent chemotactic factor derived from epithelial cells was identified as fibronectin. In addition, it was certified that the fibronectin-directed mast cell chemotaxis could be controlled by the Src family tyrosine kinase, Lyn, during the intracellular signal transduction. It will be the future problem how we should manage for the mast cell chemotaxis in the allergic airway disease such as bronchial asthma.

在包括支气管哮喘在内的过敏性疾病中，支气管粘膜中的气道肥大细胞可能通过释放多种介质和细胞因子参与了这些疾病的发病机制。虽然气道肥大细胞和支气管周围纤维化之间的关系最近已被强调，它是不知道的气道粘膜中的哪种类型的细胞能够释放趋化因子的肥大细胞。本研究通过体外培养支气管上皮细胞、成纤维细胞和血管内皮细胞，检测其培养上清条件培养液的趋化活性。结果表明，支气管上皮细胞、成纤维细胞和血管内皮细胞均能释放对肥大细胞的趋化活性，其中纤维连接蛋白是一种来源于上皮细胞的强趋化因子。此外，研究证实，纤维连接蛋白介导的肥大细胞趋化性在细胞内信号转导过程中可由Src家族酪氨酸激酶林恩控制。如何调控肥大细胞趋化性在支气管哮喘等过敏性气道疾病中的作用将是今后研究的重点。

项目成果

Suzuki T, Shoji S, Yamamoto K, Nada S, Okada M, Yamamoto T, Honda Z: "Essential roles of Lyn in fibronectin-mediated filamentous actin assembly and cell motility in mast cells."Journal of Immunology. 161. 3694-3701 (1998)

Suzuki T、Shoji S、Yamamoto K、Nada S、Okada M、Yamamoto T、Honda Z：“Lyn 在肥大细胞中纤连蛋白介导的丝状肌动蛋白组装和细胞运动中的重要作用。”免疫学杂志。

Suzuki T,Shoj S etal.: "Essential roles of Lyn in fibronectin-mediated filamentous aclin assembly and cell motility in mast cells"Journal of Immunology. 161. 3694-3701 (1998)

Suzuki T、Shoj S 等人：“Lyn 在肥大细胞中纤连蛋白介导的丝状肌动蛋白组装和细胞运动中的重要作用”免疫学杂志。

庄司俊輔: "気道粘膜と免疫-気道粘膜の防御機構と修復機序-" 呼吸. 16・2. 146-153 (1997)

Shoji俊介：“呼吸道粘膜和免疫-气道粘膜的防御机制和修复机制”16・2（1997）。