Molecular biological approarch for motor neuron disease

运动神经元病发病机制的分子生物学研究尚不清楚。我们通过VNTRs的多态存在和合成的DNA探针杂交细胞骨架蛋白，如神经丝、微管相关蛋白和tau来研究这一问题。我们选择了四个VNTR探针进行本研究。按常规方法从静脉穿刺液中洗脱DNA。采用聚合酶链式反应、杂交法等方法进行研究。遗憾的是，这些实验并没有得到有意义的结果。我们对这些结果进行了如下分析：1.运动神经元疾病的发病机制与遗传方式无关。前角细胞上的神经丝等细胞性异常不是神经丝的遗传改变所致。对于这些分析，VNTRs是粗略的基因组探针4。对于我们来说，重要的是用另一种方法进行研究，如使用微卫星，并检查另一种与轴突流动相关的蛋白质的基因组变化。我们将继续这项研究，以阐明运动神经元病的发病机制。

Molecular biological approrch for pathogenesis of motor neuron disease was still obscure. We investigated for this probrem by the presence of polymorphism of VNTRs and hyblidization with systhethized DNA probe for cytoskeletal proteins, such as neurofilament, microtubule associated proteins, and tau. We selected thrity four VNTR probes for this reserch. DNA was eluted from the venipunctured lymphocytes according to conventional methods. PCR methods, hybridization methods were applyed for these investigations. Unfortunatery, we could not get significant results by these experiments.We analyzed these result as follows ;1. Pathogenesis of motor neuron disease was not related genetic fashion.2. Cytoskletal abnormalities such as neurofilaments on the anterior spinal horn cells were not due to genetic alterations on neurofilament.3. VNTRs were rough genomic probes for these analysis4. It is important for us to investigate by another methods such as using microsattelite, and check genomic alterations in another proteins related axonal flow. We are going to continue this investigation to clarify pathogenesis of motor neuron disease.