Molecular biological approarch for motor neuron disease

运动神经元疾病的分子生物学方法

• 批准号: 05670571
• 负责人: HASEGAWA Kazuko
• 金额: $ 1.22万
• 依托单位: Kitasato University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670571/
• 关键词: motor neuron disease; VNTR; cytoskelton; 分子生物学的アプローチ

Molecular biological approrch for pathogenesis of motor neuron disease was still obscure. We investigated for this probrem by the presence of polymorphism of VNTRs and hyblidization with systhethized DNA probe for cytoskeletal proteins, such as neurofilament, microtubule associated proteins, and tau. We selected thrity four VNTR probes for this reserch. DNA was eluted from the venipunctured lymphocytes according to conventional methods. PCR methods, hybridization methods were applyed for these investigations. Unfortunatery, we could not get significant results by these experiments.We analyzed these result as follows ;1. Pathogenesis of motor neuron disease was not related genetic fashion.2. Cytoskletal abnormalities such as neurofilaments on the anterior spinal horn cells were not due to genetic alterations on neurofilament.3. VNTRs were rough genomic probes for these analysis4. It is important for us to investigate by another methods such as using microsattelite, and check genomic alterations in another proteins related axonal flow. We are going to continue this investigation to clarify pathogenesis of motor neuron disease.

运动神经元病发病机制的分子生物学基础尚不清楚。我们通过VNTRs多态性的存在以及与合成的细胞骨架蛋白如神经丝、微管相关蛋白和tau蛋白的DNA探针杂交来研究这个问题。我们选用了34种VNTR探针进行研究。根据常规方法从静脉穿刺的淋巴细胞洗脱DNA。应用PCR、杂交等方法对这些基因进行了研究。遗憾的是，这些实验并没有得到有意义的结果。运动神经元病的发病与遗传方式无关.脊髓前角细胞的细胞骨架异常如神经丝的出现不是由于神经元的遗传改变. VNTR是用于这些分析的粗略基因组探针4。因此，通过其他方法如微卫星技术进行研究，并检测与轴突流动相关的其他蛋白质的基因组改变对我们来说是很重要的。我们将继续这项研究，以阐明运动神经元疾病的发病机制。