Abnormality of free radical scavenging mechanism in skin fibroblasts with dementia of the Alzheimer type

阿尔茨海默型痴呆皮肤成纤维细胞自由基清除机制异常

• 批准号: 05670558
• 负责人: URAKAMI Katsuya
• 金额: $ 1.41万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670558/
• 关键词: Dementia of the Alzheimer type; Superoxide dismutase; Skin fibroblast; SOD; catalase; glutathion peroxidase; superoxide dismutase

We examined superoxide dismutase (SOD) mRNA in skin fibroblasts with dementia of the Alzheimer type (DAT). THE DAT patients were divided into three subgroups as follows : Group A : patients that developed rapidly ; Group B : patients that developed gradually ; Group C : patients that developed very slowly. We determined SOD mRNA levels by Northern blot analysis. SOD mRNA level in skin fibroblast decreased in DAT, especially Group A.The change of SOD mRNA was closely correlate with clinical course of DAT.The determination of SOD mRNA in skin fibroblast may be useful for the diagnosis of three subgroups of DAT.

我们检测了阿尔茨海默型痴呆（DAT）皮肤成纤维细胞中超氧化物歧化酶（SOD）mRNA。DAT患者分为三个亚组，如下：A组：快速发展的患者; B组：逐渐发展的患者; C组：发展非常缓慢的患者。用北方印迹法测定SOD mRNA水平。DAT患者皮肤成纤维细胞SOD mRNA水平降低，以A组为甚，其变化与DAT的临床病程密切相关，检测皮肤成纤维细胞SOD mRNA水平可作为DAT三种亚型的诊断指标。

项目成果

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Urakami K 等人：“利用脑脊液中乙酰胆碱酯酶和生长抑素水平表征阿尔茨海默型老年痴呆的病程”Acta Neurol Scand。

Urakami K et al: "Amyloid beta protein precursor with leunitz-type protease inhibitor domain CAPPI1 in cerebrospinal fluid and APPI mRNAs in cultured skin fibroplast of patients" Tohoku J Exp Med. 174. 199-207 (1994)

Urakami K 等人：“脑脊液中具有 leunitz 型蛋白酶抑制剂结构域 CAPPI1 的淀粉样 β 蛋白前体和患者培养的皮肤纤维细胞中的 APPI mRNA”Tohoku J Exp Med。

Okada A: "Expression of amyloid beta-protein precursor mRNAs in cultured skin fibroblasts taken from patients with dementia of the Alzheimertype." Dementia. 5. 55-56 (1994)

Okada A：“从阿尔茨海默型痴呆患者身上培养的皮肤成纤维细胞中淀粉样蛋白 β 蛋白前体 mRNA 的表达。”

浦上克哉他: "Alzluimer型痴呆患者皮膚線維芽細胞におけうlysosome機能には異常がない" 神経内科. 39. 650-652 (1993)

Katsuya Urakami 等人：“Alzluimer 痴呆患者皮肤成纤维细胞的溶酶体功能没有异常”《神经病学》39. 650-652 (1993)。

Urakami K et al: "Cu Zu superoxide dismutase in patients with dementia of the Alzheimer type" Acta Neurol Scand. 91. 165-168 (1995)

Urakami K 等人：“阿尔茨海默型痴呆患者中的 Cu Zu 超氧化物歧化酶”Acta Neurol Scand。