ROLE OF ENDOTHELIUM DERIVED RELAXING FACTOR IN THE REGULATION OF THE TONE OF THE VENOUS SYSTEM

内皮源性松弛因子在静脉系统张力调节中的作用

• 批准号: 05670601
• 负责人: MINATOGUCHI Shinya
• 金额: $ 1.15万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670601/
• 关键词: dopamine; EDRF; alpha2-adrenoceptors; Dopamaine; 摘出股動・静脈; 内皮α_2受容体; ACE阻害剤; Acetylcholine; Indomethacin; 摘出大腿動・静脈; Prostaglandin F_2α; 内皮由来血管拡張物質; Nitric Oxide; L-nitroarginine

[Purpose]Dopamine has been widely used for the treatment of congestive heart failure, However, effects of dopamine on the vascular system have not yet been fully clarified. In this study, we investigated whether the constriction of the canine isolated femoral arterie and veins are modified by the endothelium derived relaxing factor (EDRF) which is released via the stimulation of alpha2 -adrenoceptors in the endothelium.[Method]Canine isolated femoral arteries and veins which were cut into 5 mm long ring segments with and without endothelium were suspended in 37ﾟC Krebs-Henseleit solution, and the changes in isometric force were recorded.[Results and Discussion](1)alpha2-agonist, clonidine (1X10 - 1X10 M) constricted only the veins, but not the arterles. Pretreatment with NOS inhibitor, NG-nitro-L-arginine (L-NA : 1X10 M) potentiated the constriction of the vein, but not of the arteries, exerted by clonidine, suggesting that this potentiation of the construction of the veins was mediate … More d through the production of EDRF via the activation of alpha2-adrernoceptors. (2) Dopamine (1X10 - 1X10 M) constricted both the arterie and veins. This constriction was potentiated by the pretreatment with L-NA (1X10 M), suggesting that dopamine releases EDRF in both the arteries and veins. (3) Removal of endothelium potentiated the dopamine-induced constriction of the arteries and veins, however, after the pretreatment with L-NA (1X10 M), this constriction was not observed.(4) Dopamine constricted the veins, although pretreatment with alpha2-antagonist, yohimbine((1X10 M) partially inhibited the constriction of the vein exerted by dopamine. Pretreatment with both yohimbine and L-NA did not affect the constriction of the vein exerted by dopamine. Pretreatment with yohimbine or both yohimbine and L-NA did not affect the constriction of the arteries exerted by dopamine. These results suggest that dopamine constricts both the arteries and veins and that this constriction is modified by the vasodilatory action of EDRF released via the activation of alpha2-adrenoceptors.[Conclusion]It is suggested that dopamine releases EDRF via the activation of alpha2-adrenoceptors on the endothelium in the canine isolated femoral arteries and veins. Less

[目的]多巴胺已被广泛用于治疗充血性心力衰竭，但其对血管系统的影响尚未完全阐明。在本研究中，我们观察了内皮源性松弛因子是否能改善犬离体股动脉和股静脉的收缩。[方法]犬离体股动脉和静脉切成5 mm长的有内皮和不带内皮的环状，悬浮在37ﾟC Krebs-Henseleit溶液中，记录等长力的变化。[结果和讨论](1)α2-激动剂可乐定(1X10-1X10M)只收缩静脉，不收缩动脉。一氧化氮合酶抑制剂NG-硝基-L-精氨酸(L-NA：1X10 M)可增强可乐定对静脉的收缩作用，但不能增强可乐定对动脉的收缩作用，提示这种增强静脉结构的作用是介导…更多的d通过激活α2-肾上腺素能受体来产生EDRF。(2)多巴胺(1×10~1×10~(-1)M)收缩动脉和静脉。这种收缩作用可被L-NA(1X10M)所增强，表明多巴胺在动脉和静脉中都能释放EDRF。(3)去内皮可增强多巴胺引起的血管收缩，但经L-NA(1×10 M)处理后，这种收缩作用不明显。(4)多巴胺收缩血管，但用α2受体拮抗剂育亨宾(1×10 M)可部分抑制多巴胺引起的血管收缩。育亨宾和L-纳均不影响多巴胺的收缩作用。育亨宾或育亨宾与L-纳合用均不影响多巴胺引起的动脉收缩。这些结果表明，多巴胺收缩动脉和静脉，这种收缩作用可被激活α2-肾上腺素能受体释放的EDRF的血管扩张作用所改变。[结论]在犬股动脉和股静脉中，多巴胺通过激活内皮上的α2-肾上腺素能受体释放EDRF。较少

项目成果

H.Ito, S.Minatoguchi, et al: "Baroreflex Modifies the Effect of Vasodilators on Systemic Capacitance Vessel in Dogs" Veins: Their Functional Role in the Circulation. 79-89 (1993)

H.Ito、S.Minatoguchi 等人：“Baroreflex 改变血管扩张剂对狗全身电容血管的影响”静脉：它们在循环中的功能作用。

S.Minatoguchi, H.Ito et al: "Modulation of noradrenaline release through presynaptic a2-adrenoceptors in congestive heart failure" Am Heart J. 516-521 (1995)

S.Minatoguchi、H.Ito 等人：“充血性心力衰竭中通过突触前 α2-肾上腺素受体调节去甲肾上腺素释放”Am Heart J. 516-521 (1995)

S.Minatoguchi,H.Ito et al: "Modulation of noradrenaline release through presynaptic α_2-adrenoceptors in congestive heart failure" American Heart Journal. 516-521 (1995)

S. Minatoguchi、H. Ito 等人：“充血性心力衰竭中通过突触前 α_2-肾上腺素受体调节去甲肾上腺素释放”美国心脏杂志 516-521 (1995)。

S.Minatoguchi, H.Ito, et al: "Modulation of noradrenaline release through presynaptic α_2-adrenoceptors in congestive heart failure" American Heart Journal. 516-521 (1995)

S.Minatoguchi、H.Ito 等人：“充血性心力衰竭中通过突触前 α_2-肾上腺素受体调节去甲肾上腺素释放”美国心脏杂志 516-521 (1995)。

H.Ito, S.Minatoguchi et al: "Baroreflex modifies the effect of vasodilators on systemic capacitance vessel in dogs. In Veins" Their Functional Role in the Circulation. 79-89 (1993)

H.Ito、S.Minatoguchi 等人：“Baroreflex 改变了血管扩张剂对狗全身电容血管的影响。在静脉中”它们在循环中的功能作用。