Distribution and function of phosphoinositide second messenger system in developing brain

磷酸肌醇第二信使系统在大脑发育中的分布和功能

• 批准号: 05670659
• 负责人: MIZUGUCHI Masashi
• 金额: $ 1.28万
• 依托单位: National Institute of Neuroscience, NCNP
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670659/
• 关键词: Second messenger; Inositol 1,4,5-trisphosphate; Cyclic ADP-ribose; CD38; Calcium ion; Immunohistochemistry; Central nervous System; イノシトールリン脂質; 細胞内情報伝達

A.Studies on the phosphoinositide-mediated system.Developmental changes of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated in rat cerebellum by immunoelectron microscopy. An important role of this system during development was suggested by the high expression of IP3K observed on structures having special developmental significance, such as the synapses between the prarllel fibers and the proximal dendrites of Purkinje cells.B.Studies on the cyclic ADP-ribose (cADPR) -mediated system.The expression in brain of CD38 antigen, which is involved in both the formation and hydrolysis of cADPR,was investigated with polyclonal antibodies raised against synthetic CD38 peptide fragments. Using these antibodies in addition to monoclonals, the presence of CD38 in brain was demonstrated by both immunochemical and immunohistochemical methods.

A.磷酸肌醇介导系统的研究。通过免疫电镜研究大鼠小脑肌醇1,4,5-三磷酸3-激酶(IP3K)的发育变化。在具有特殊发育意义的结构上观察到的 IP3K 高表达表明了该系统在发育过程中的重要作用，例如浦肯野细胞的平行纤维和近端树突之间的突触。 使用针对合成 CD38 肽片段的多克隆抗体进行研究。除了单克隆抗体外，还使用这些抗体，通过免疫化学和免疫组织化学方法证明了大脑中 CD38 的存在。

项目成果

水口 雅: "組織培養を用いた神経系の発生と老化に関する研究-ホスホリパーゼCアイソザイムの中枢神経系培養細胞における分布-" 笹川医学医療記念財団・海外派遣研究者報告. 21-25 (1993)

Masaru Mizuguchi：“利用组织培养研究神经系统的发育和老化-中枢神经系统培养细胞中磷脂酶C同工酶的分布-”笹川医学和医学纪念基金会海外研究员报告21-25（1993）。

山田光則: "Developmental profile of inositol 1,4,5‐trisphosphate 3‐kinase in rat cerebella cortex: light and electron microscopic immunohistochemical studies." Developmental Brain Research. 71. 137-145 (1993)

Mitsunori Yamada：“大鼠小脑皮质中肌醇 1,4,5-三磷酸 3-激酶的发育概况：光和电子显微镜免疫组织化学研究。发育脑研究”。

水口 雅: "先天性代謝異常症" 日本臨床. 51. 2919-2923 (1993)

Masaru Mizuguchi：“先天性代谢缺陷”日本临床 51. 2919-2923 (1993)

Yamada,M.et al.: "Specific expression of inositol 1,4,5-trisphosphate 3-kinase in the dendritic spines." Brain Pathology. 4. 383 (1994)

Yamada,M.et al.：“肌醇 1,4,5-三磷酸 3-激酶在树突棘中的特异性表达。”

Mizuguchi,M.et al.: "Expression of Bcl-2 protein in murine neural cells in culture." Brain Research. 649. 197-202 (1994)

Mizuguchi,M.et al.：“培养的小鼠神经细胞中 Bcl-2 蛋白的表达。”