Distribution and function of phosphoinositide second messenger system in developing brain

A.肌醇磷脂介导系统的研究免疫电子显微镜观察了大鼠小脑内肌醇1，4，5-三磷酸3-激酶(IP3K)的发育性变化。在具有特殊发育意义的结构上观察到IP3K的高表达，提示了该系统在发育过程中的重要作用。B.环状ADP-核糖(CADPR)介导的系统的研究。利用合成的CD38肽片段的多克隆抗体，研究CD38抗原在脑内的表达。免疫组织化学和免疫组织化学方法均证实CD38在脑内的存在。

A.Studies on the phosphoinositide-mediated system.Developmental changes of inositol 1,4,5-trisphosphate 3-kinase (IP3K) were investigated in rat cerebellum by immunoelectron microscopy. An important role of this system during development was suggested by the high expression of IP3K observed on structures having special developmental significance, such as the synapses between the prarllel fibers and the proximal dendrites of Purkinje cells.B.Studies on the cyclic ADP-ribose (cADPR) -mediated system.The expression in brain of CD38 antigen, which is involved in both the formation and hydrolysis of cADPR,was investigated with polyclonal antibodies raised against synthetic CD38 peptide fragments. Using these antibodies in addition to monoclonals, the presence of CD38 in brain was demonstrated by both immunochemical and immunohistochemical methods.