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膵潅流法によるMSG肥満マウスの膵β細胞インスリン動態に関する研究

胰腺灌注法研究味精肥胖小鼠胰腺β细胞胰岛素动力学

基本信息

批准号：
05670692
负责人：
KINUGASA Akihiko
金额：
$ 1.28万
依托单位：
KYOTO PREFECTURAL UNEVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670692/
关键词：
PANCREATIC PERFUSION OBESITY MSG INSULIN 膵灌流

项目摘要

The mechanism of the hyperinsulinemia of the monosodium glutamate-induced obese mice (MSG mice) was investigated.The male mice (Jcl : ICR strain) were rendered obese by subcutaneous injection of 10% monosodium L-glutamate (MSG), 2 mg/g of weight every 24 hour, for the first five postnatal days. Blood glucose and plasma insulin levels were measured at fasting and at feeding ad libitum on 4,8,12,24, and 36 weeks of age. Glucose tolerance tests and arginine tolerance tests were performed at 8 and 24 weeks of age. The pancreas perfusions were performed according to the method of Grodsky et al. The insulin secrition was stimulated with 30 mM glucose and 19 mM arginine, and insulin in the perfusate were measured at an interval of one minute.At 4 weeks of age, plasma insulin level at feeding ad libitum were slightly higher in the MSG mice compared to the control mice. Marked elevation of the plasma insulin level was observed in fed MSG mice after 12 weeks of age. The MSG mice revealed a marked increase in insulin secretion by arginine tolerance test at 8 weeks of age. On the pancreas perfusion study, intact insulin response to both blucose and arginine was maintained in the MSG mice.These results suggest that the hyperinsulinemia of the MSG mice may be caused by the disorder in neurological regulation.

研究了谷氨酸钠致肥胖小鼠高胰岛素血症的发生机制。雄性小鼠（Jcl: ICR品系）在出生后5天皮下注射10%的l -谷氨酸钠（MSG），每24小时注射2 mg/g体重。分别在4、8、12、24和36周龄时测定空腹和自由采食时的血糖和血浆胰岛素水平。葡萄糖耐量试验和精氨酸耐量试验分别在8周龄和24周龄进行。胰腺灌注参照Grodsky等的方法。用30 mM葡萄糖和19 mM精氨酸刺激胰岛素分泌，每隔1分钟测定灌注液中的胰岛素。在4周龄时，与对照小鼠相比，随意喂食味精小鼠的血浆胰岛素水平略高。12周龄后，喂食味精的小鼠血浆胰岛素水平明显升高。精氨酸耐量试验显示，味精小鼠在8周龄时胰岛素分泌明显增加。在胰腺灌注研究中，味精小鼠对葡萄糖和精氨酸的胰岛素反应保持完整。这些结果提示，味精小鼠的高胰岛素血症可能是由神经调节紊乱引起的。