Studies on in vivo binding ability of anti-nuclear antibodies to epidermal cell nucleus

抗核抗体体内表皮细胞核结合能力的研究

• 批准号: 05670729
• 负责人: FURUKAWA Fukumi
• 金额: $ 1.47万
• 依托单位: Hamamatsu Univ., School of Med., Dept.of Dermatol.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670729/
• 关键词: MRL mouse; lupus erythematoses; anti-nuclear antibody; MCTD; Skin; 抗RNP抗体; ループスエリテマトーヂス; 培養細胞; モデルマウス

The mechanisms of epidermal nuclear staining (ENS) and its relation to skin lesions are still controversial and unclear. We tried to find spontaneous autoimmune prone model mice with ENS in order to better understand the pathological mechanisms. Our results are as follows ;1.We first demonstrated the epidermal nuclear staining by direct immunofluorescence in aged MRL/Mp-+/+ mice. This positive staining was correlated with the positive nuclear staining of kidney in examined mice.2.Spontaneous skin lesions appeared in the age-dependent manner. Light microscopy showed little lymphocytes infiltration, compared with that of SLE-prone MRL/lpr mic.3.Epidermal nuclear staining was not correlated with macroscopic skin lesions.4.In female aged MRL/n mice, there was the positive association between epidermal nuclear staining and anti-RNP antibodise.These results suggested thatged MRL/n mice provide unique models for investigating the mechanisms of epidermal nuclear staining by direct immunofluorescence technique.

表皮细胞核染色(ENS)的机制及其与皮肤损害的关系仍存在争议和不清楚。为了更好地了解ENS的病理机制，我们试图寻找自发性自身免疫倾向的ENS模型小鼠。我们的研究结果如下：1.首次用直接免疫荧光法显示了老年MRL/MP-+/+小鼠的表皮细胞核染色。这一阳性染色与受检小鼠肾脏的阳性核染色呈正相关。2.自发性皮肤损害以年龄依赖性的方式出现。光镜下，与SLE易感的mrl/lpr小鼠相比，mrl/n小鼠的淋巴细胞浸润较少。3.表皮细胞核染色与肉眼可见的皮损无关。4.在雌性老年mrl/n小鼠中，表皮核染色与抗RNP抗体呈正相关。

项目成果

K.Ikai: "Immunohistochemical localization of lipocortins in normal and psoriatic human skin" Arch.Dermatol Res. 285. 296-299 (1993)

K.Ikai：“脂皮质素在正常和银屑病人类皮肤中的免疫组织化学定位”Arch.Dermatol Res。

Furukawa, F.et al.: "Pathogenesis of lupus dermatoses in autoimmune mice. XIX.Attempts to induce subepidermal immunoglobulin deposition in MRL/Mp-+/+ mice." Arch Dermatol Res.285. 20-26 (1993)

Furukawa, F.等人：“自身免疫小鼠狼疮皮肤病的发病机制。XIX.尝试在 MRL/Mp-/ 小鼠中诱导表皮下免疫球蛋白沉积。”

Kanauchi,H: "Evaluation of the Japanese-Chinese Herbal Medicine,Kampo,for the treatment" J Dermatol. 21. 935-939 (1994)

Kanauchi,H：“日中草药汉方治疗的评价”J Dermatol。

Furukawa,F.: "Pathogenesis of lupus dermatoses in autoimmune mice.XIX.Attempts to induce subepidermal immunoglobulin deposition in MRL/MPMp_<-+/+> mice." Arch Dematol Res. 285. 20-26 (1993)

Furukawa,F.：“自身免疫小鼠狼疮皮肤病的发病机制。XIX。尝试在 MRL/MPMp_<-/> 小鼠中诱导表皮下免疫球蛋白沉积。”

古川福実: "アレルギー産業環境からのアプローチ" 金原出版, 368 (1993)

古川福美：“来自过敏行业环境的方法”金原出版社，368（1993）