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Susceptihility of human keratinocytes to ultraviolet B light

人角质形成细胞对紫外线 B 光的敏感性

基本信息

批准号：
07670940
负责人：
FURUKAWA Fukumi
金额：
$ 1.47万
依托单位：
Hamamatsu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670940/
关键词：
ultraviolet light keratinocyte skin SS-A Ro RNP susceptibility ループスエリテマトーデスサンスクリーンサイトカイン ETAF ループスエリテマトーデス SS A

项目摘要

Susceptibility of human keratinocytes to ultraviolet B light (UVBL) was examined in order to understand the mechanisms of photosensitivity found in lupus erythematosus (LE) and other photosensitive diseases.1.Keratinocytes cultureCulture of human keratinocytes was established from patients with photosensitivity and from normal controls by using suction blister epidermal roof and serum-free keratinocyte growth medium.2.UVBL-induced cytotoxicity of keratinocytesUVBL induced cytotoxicity of cultured keratinocytes at the dose of 50 and 100 mJ/cm^2. The cytotoxicity was much higher in photosensitivity patients than normal controls. Apoptotic cell ratio was similar between patients and normal controls.3.UVBL-induced susceptibility of keratinocytesThe usefulness of expression of U1RNP and SS-A/Ro antigens was examined. These antigens were induced on the surface of UVBL-irradiated keratinocytes, which was confirmed by an immunofluorescence method and a confocal laser microscopy. Positive binding of antisera containing antibodies against U1RNP or SS-A/Ro was much higher in patients with photosensitivity than normal controls.4.Regulations of photosusceptibility by sunscreen and cytokinesUVBL-induced susceptibility was blocked by sunscreen, and upregulated by ETAF.These findings suggest that expression of U1RNP or SS-A/Ro antigens by UVBL was usefull for the detection of photosusceptibility as well as photosensitivity.

研究人角质形成细胞对紫外B光（UVBL）的敏感性，以了解红斑狼疮（LE）和其他光敏性疾病中光敏性的机制。 1.角质形成细胞培养采用吸泡表皮屋顶和正常对照的方法建立人角质形成细胞的培养物。 2.UVBL诱导的角质形成细胞细胞毒性UVBL在50和100 mJ/cm^2剂量下对培养的角质形成细胞产生细胞毒性。光敏性患者的细胞毒性远高于正常对照。患者与正常对照之间的凋亡细胞比例相似。3.UVBL诱导的角质形成细胞的敏感性检测U1RNP和SS-A/Ro抗原表达的有效性。这些抗原在 UVBL 照射的角质形成细胞表面被诱导，并通过免疫荧光方法和共焦激光显微镜证实。光过敏患者中含有 U1RNP 或 SS-A/Ro 抗体的抗血清的阳性结合率远高于正常对照。 4.防晒霜和细胞因子对光敏感性的调节 UVBL 诱导的敏感性被防晒霜阻断，并被 ETAF 上调。这些发现表明 UVBL 表达 U1RNP 或 SS-A/Ro 抗原有助于检测光敏感性和光敏性。