Keratinocyte-derived auto antigens involved in systemic autoimmune diseases

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1. Anti-SS-A/Ro antibody is bonding to the cell surface of keratinocytes irradiated with ultraviolet B light, which was confirmed by confocal microscopy.2. Autoantibody dependency was confirmed in the cytotoxicity of UVB irradiated keratinocytes of patients plus patients' sera plus mononuclear cells from normal individuals.3. Isolations of nucleosides and autoantigens were performed in the culture supernatants of patients' keratinocytes irradiated with UVB light. Nucleosomes were partially purified and were immunized to MRL/n mice. In these mice, low titer of anti-nucleosome antibody was detected.4. Mononuclear cells from patients with high titer of anti-SS-A/Ro antibody were transferred to SCID mice in order to induce the skin lesions. Our data showed that transferred mice produced slight erythema and hair loss without immunoglobulin and/or complement components depositions at dermoepidermal junction.5. Signal transduction systems are now under investigations.