Studies on autoimmune alopecia in New Zealand Black/KN mouse

自身免疫性脱发是系统性红斑狼疮(SLE)的主要症状之一。虽然已经提出了体内免疫复合物沉积和单个核细胞浸润的原因，但其机制仍不清楚。雄性新西兰黑(NZB)/KN小鼠是一种新的SIR易感小鼠，与关节炎有关，这是自发性脱发皮损的特征。本研究以NZB/KN(H-2^d)、标准SLE易感NZB/N(H-2^d)、对照NZ White(NZW)/SK(H-2^d)小鼠为研究对象，设计了NZB/KN(H-2^d)小鼠自身免疫性脱发的遗传学研究。饲养(NZW/SK×NZB/KN)(H-2^&lt；z/d&gt；)F1杂交小鼠92只(H-2^&lt；d/d&gt；，H-2^&lt；z/d&gt；H-2^&lt；z/z&gt；)。在F2小鼠中，雄性NZB/KN小鼠在3个月龄和12个月龄时分别有25%和80%的小鼠上背部和尾部出现无红斑的脱发皮损。早期脱发皮损显示少量…。更多的f渗入毛囊周围。NZB/N和NZW/SK小鼠均未发现脱发皮损。雄性和雌性F1小鼠的脱发发生率分别为13.3%和6.7%。F2在44%的雄性小鼠和7.3%的雌性小鼠中出现脱发。脱发与男性之间存在较强的正相关关系，而脱发、女性与H-2复合体之间没有明显的正相关关系。12月龄NZB/KN小鼠毛囊基底膜有Ig M和Ig 0沉积，分别为75%和5%，而雄性NZB/N和NZW/SK小鼠则为0。雄性F2小鼠Ig M沉积阳性率为53%，雌性F2小鼠Ig沉积阳性率为33%。NZB/KN小鼠自身免疫性脱发与Y染色体相关抗原(Yalphapha)密切相关，而与H-2复合体或体内Ig沉积无关。由于其他SLB易感小鼠如NZB/N和MRL/LPR小鼠的狼疮皮肤病受H-2复合体或IPR基因的调控，因此自身免疫性脱发的发病机制可能不同于红斑及其相关的免疫复合体沉积。较少

Autoimmune alopecia is one of the major symptoms in systemic lupus erythematosus(SLE). Although the in vivo immune complexes deposits and mononuclear cell infiltrates have been proposed as the cause, the mechanisms are still obscure. A male New Zealand Black (NZB)/KN mouse is a novel SIR-prone mouse associated with arthritis, which is characteristic in spontaneous alopecia lesions. We designed the genetic studies on autoimmune alopecia in NZB/KN mice in relation to several autoimmune traits.NZB/KN(H-2^d), the standard SLE-prone NZB/N(H-2^d), the control NZ White (NZW)/sk (H-2^d) mice were purchased and bred in our laboratory. Fl hybrid mice of (NZW/sk x NZB/KN) (H-2^<z/d>) and 92 F2 mice of (F1x F1) (H-2^<d/d>, H-2^<z/d>, H-2^<z/z>) were raised. In F2 mice, the correlation studies were performed.Male NZB/KN mice showed alopecia lesions without erythema on the upper back and the tail regions in 25% and 80% at the age of 3 mo and 12 mo. The early alopecia lesions showed a slight number o … More f infiltrates around hair follicles. No alopecia lesions were found in NZB/N or NZW/sk mice. Male and female Fl mice showed alopecia in 13.3% and 6.7%. F2 developed alopecia in 44% of male mice and 7.3% of female mice. Strong positive association was found between alopecia and male, but not among alopecia, female and H-2 complex. Ig M and Ig 0 deposits at hair follicle basement membrane were found in 75% and 5% of 12- mo-old NZB/KN mice, and in zero of male NZB/N mice or NZW/sk mice. Male F2 mice showed 53% positivity of Ig M deposits and 33% of Ig G deposits, and female F2 showed similar incidences of Ig deposits. No associations were found among alopecia, Ig deposits, H-2 complex and proteinuria.These results suggest that autoimmune alopecia in NZB/KN mice are closely associated with Y chromosome associated antigens (Yalphaalpha), but not with H-2 complex or in vivo Ig deposits. Since lupus dermatoses in other SLB-prone mice such as NZB/N and MRL/lpr mice are regulated by H-2 complex or Ipr gene, it is likely that the pathogenesis of autoimmune alopecia is different from that of erythema and the related immune complex deposits. Less