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Studies on autoimmune alopecia in New Zealand Black/KN mouse

新西兰Black/KN小鼠自身免疫性脱发的研究

基本信息

批准号：
09670873
负责人：
FURUKAWA Fukumi
金额：
$ 1.92万
依托单位：
Hamamatsu University school of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670873/
关键词：
mouse lupus erythematosus alopecia sex H-2 autoimmune NZBマウス

项目摘要

Autoimmune alopecia is one of the major symptoms in systemic lupus erythematosus(SLE). Although the in vivo immune complexes deposits and mononuclear cell infiltrates have been proposed as the cause, the mechanisms are still obscure. A male New Zealand Black (NZB)/KN mouse is a novel SIR-prone mouse associated with arthritis, which is characteristic in spontaneous alopecia lesions. We designed the genetic studies on autoimmune alopecia in NZB/KN mice in relation to several autoimmune traits.NZB/KN(H-2^d), the standard SLE-prone NZB/N(H-2^d), the control NZ White (NZW)/sk (H-2^d) mice were purchased and bred in our laboratory. Fl hybrid mice of (NZW/sk x NZB/KN) (H-2^<z/d>) and 92 F2 mice of (F1x F1) (H-2^<d/d>, H-2^<z/d>, H-2^<z/z>) were raised. In F2 mice, the correlation studies were performed.Male NZB/KN mice showed alopecia lesions without erythema on the upper back and the tail regions in 25% and 80% at the age of 3 mo and 12 mo. The early alopecia lesions showed a slight number o … More f infiltrates around hair follicles. No alopecia lesions were found in NZB/N or NZW/sk mice. Male and female Fl mice showed alopecia in 13.3% and 6.7%. F2 developed alopecia in 44% of male mice and 7.3% of female mice. Strong positive association was found between alopecia and male, but not among alopecia, female and H-2 complex. Ig M and Ig 0 deposits at hair follicle basement membrane were found in 75% and 5% of 12- mo-old NZB/KN mice, and in zero of male NZB/N mice or NZW/sk mice. Male F2 mice showed 53% positivity of Ig M deposits and 33% of Ig G deposits, and female F2 showed similar incidences of Ig deposits. No associations were found among alopecia, Ig deposits, H-2 complex and proteinuria.These results suggest that autoimmune alopecia in NZB/KN mice are closely associated with Y chromosome associated antigens (Yalphaalpha), but not with H-2 complex or in vivo Ig deposits. Since lupus dermatoses in other SLB-prone mice such as NZB/N and MRL/lpr mice are regulated by H-2 complex or Ipr gene, it is likely that the pathogenesis of autoimmune alopecia is different from that of erythema and the related immune complex deposits. Less

自身免疫性脱发是系统性红斑狼疮（SLE）的主要症状之一。虽然体内免疫复合物沉积和单核细胞浸润被认为是病因，但其机制尚不清楚。一只雄性新西兰黑(NZB)/KN小鼠是一种与关节炎相关的新型sir易感小鼠，关节炎是自发性脱发病变的特征。我们设计了NZB/KN小鼠自身免疫性脱发与几种自身免疫性性状的遗传研究。购买NZB/KN（H-2^d）、标准鼠NZB/N（H-2^d）、对照鼠NZ White (NZW)/sk （H-2^d）在实验室饲养。饲养（NZW/sk × NZB/KN）（H-2^<z/d>）的Fl杂交小鼠和（F1x F1）（H-2^<d/d>、H-2^<z/d>、H-2^<z/z>）的F2小鼠92只。对F2只小鼠进行相关性研究。雄性NZB/KN小鼠在3月龄和12月龄时，有25%和80%的小鼠出现上背部和尾部无红斑的秃发病变。早期秃发病变在毛囊周围有少量浸润。NZB/N和NZW/sk小鼠均未见脱发病变。雄性和雌性小鼠脱发率分别为13.3%和6.7%。F2在44%的雄性小鼠和7.3%的雌性小鼠中出现脱发。脱发与男性呈显著正相关，而脱发与女性与H-2复合物无显著正相关。12龄NZB/KN小鼠毛囊基底膜Ig M和Ig 0沉积率分别为75%和5%，雄性NZB/N小鼠和NZW/sk小鼠均为零。雄性F2小鼠Ig M和Ig G的阳性率分别为53%和33%，雌性F2小鼠Ig M和Ig G的阳性率相似。脱发、Ig沉积、H-2复合物和蛋白尿之间无相关性。这些结果表明，NZB/KN小鼠自身免疫性脱发与Y染色体相关抗原（yalpha - α）密切相关，但与H-2复合物或体内Ig沉积无关。由于NZB/N、MRL/lpr等其他slb易感小鼠狼疮皮肤病受H-2复合物或Ipr基因调控，自身免疫性脱发的发病机制可能与红斑及相关免疫复合物沉积的发病机制不同。少