Study On The Role Of Growth Factors Factors For Growth Of Extremely Low Birth Weight Infants Using Extrauterine Fetal Incubation Model System Of Goats.

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To clarify the role of growth factors on growth of extremely low birth weight infants, plasma insulin like growth factor I and II (IGF-I,II) from the fetus of the goat incubated in extrauterine arterio-venous extracorporeal membrane oxygenation (A-V ECMO) system were determined by formic acid and acetone extraction method. IGF-I levels of the fetus were increased gradually after incubation, on the other hand, IGF-II levels were decreased to the mother's level within 60 to 80 hours after beggining of incubation. These results indicate that IGF-I plays an important role on growth of newborn infants and IGF-II on growth of fetus.As the indicator of pulmonary surfactant synthesis, lecithin sphingomyelin ratio (L/S) and surfactant protein A (SP-A) levels in amniotic fluid or lung liquid were determined to evaluate the pulmonary maturation. 110-130 days of gestation, L/S ratios and SP-A of amniotic fluid were low, but those of lung liquid were increased rapidly after 60-70 hours after incubation.Plasma IGF-II levels of human cord blood and extremely low birth weight infants were determined and compared at the same postconceptional weeks. IGF-II levels in cord blood were significantly higher than those of after birth. This difference is one of the cause of growth disturbance in extremely low birth weight infants compared to intrauterine growth.These results indicate that induction of IGF-II will make it possible to establish the intrauterine growth in extremely low birth weight infants.