Study On The Role Of Growth Factors Factors For Growth Of Extremely Low Birth Weight Infants Using Extrauterine Fetal Incubation Model System Of Goats.

利用山羊宫外胎儿孵化模型系统研究生长因子对极低出生体重婴儿生长的作用。

• 批准号: 05670973
• 负责人: UETANI Yoshiyuki
• 金额: $ 1.41万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670973/
• 关键词: extremely low birth weight infants; extrauterine fetal incubation; IGF-II; IGF-I; growth factor; fetal growth; lecithin; spingomyelin ratio; surfactant protein A

To clarify the role of growth factors on growth of extremely low birth weight infants, plasma insulin like growth factor I and II (IGF-I,II) from the fetus of the goat incubated in extrauterine arterio-venous extracorporeal membrane oxygenation (A-V ECMO) system were determined by formic acid and acetone extraction method. IGF-I levels of the fetus were increased gradually after incubation, on the other hand, IGF-II levels were decreased to the mother's level within 60 to 80 hours after beggining of incubation. These results indicate that IGF-I plays an important role on growth of newborn infants and IGF-II on growth of fetus.As the indicator of pulmonary surfactant synthesis, lecithin sphingomyelin ratio (L/S) and surfactant protein A (SP-A) levels in amniotic fluid or lung liquid were determined to evaluate the pulmonary maturation. 110-130 days of gestation, L/S ratios and SP-A of amniotic fluid were low, but those of lung liquid were increased rapidly after 60-70 hours after incubation.Plasma IGF-II levels of human cord blood and extremely low birth weight infants were determined and compared at the same postconceptional weeks. IGF-II levels in cord blood were significantly higher than those of after birth. This difference is one of the cause of growth disturbance in extremely low birth weight infants compared to intrauterine growth.These results indicate that induction of IGF-II will make it possible to establish the intrauterine growth in extremely low birth weight infants.

为探讨生长因子在极低出生体重儿生长中的作用，采用甲酸-丙酮提取法测定了体外膜肺氧合（A-VECMO）系统中培养的山羊胎儿血浆中胰岛素样生长因子I、II（IGF-I、II）的含量。胎儿IGF-Ⅰ水平在孵化后逐渐升高，而IGF-Ⅱ水平在孵化开始后60 ~ 80小时内降至母体水平。结果表明，IGF-Ⅰ对新生儿的生长发育起重要作用，IGF-Ⅱ对胎儿的生长发育起重要作用，以羊水或肺液中卵磷脂/鞘磷脂比值（L/S）和肺表面活性物质蛋白A（SP-A）作为肺表面活性物质合成的指标，评价肺成熟。妊娠110-130天，羊水L/S比值和SP-A较低，而肺液L/S比值和SP-A在孕60-70小时后迅速升高。脐血IGF-Ⅱ水平明显高于生后。这种差异是极低出生体重儿与宫内生长相比生长障碍的原因之一，这些结果表明，IGF-II的诱导将使极低出生体重儿建立宫内生长成为可能。

项目成果

Hisano K,Okada M,Sakata M,Noma O,Yokoyama N,Uetani Y,Nakamura H: "Experimental study of artificial placenta ; Doppler echo cardiographic evaluation of goat fetal blood flow." Jpn J Artif Organs. 22 (3). 880-884 (1993)

Hisano K，Okada M，Sakata M，Noma O，Yokoyama N，Uetani Y，Nakamura H：“人工胎盘的实验研究；山羊胎儿血流的多普勒超声心动图评估。”

坂田雅宏: "Color Doppler法によるECMO A-V Bypass下の山羊胎児血流分布の評価-特に動脈管血流と胎児血行動態について-" 人工臓器. 23. 921-924 (1994)

Masahiro Sakata：“使用彩色多普勒方法评估 ECMO A-V 旁路下山羊胎儿的血流分布 - 特别是关于动脉导管血流和胎儿血流动力学 -”人工器官 23. 921-924 (1994)。

久野克也: "ヤギ胎仔水中保育実験におけるドップラー超音波法を用いた血行動態の研究." 人工臓器. 22. 880-884 (1993)

Katsuya Kuno：“在山羊胎儿水下孵化实验中使用多普勒超声进行血流动力学研究。”22. 880-884 (1993)。

坂田雅宏: "Color Doppler法によるECMO A-VBypass下の山羊胎児血流分布の評価 -特に動脈管血流と胎児血行動態について-." 人工臓器. 23. 921-924 (1994)

Masahiro Sakata：“使用彩色多普勒方法评估 ECMO A-VBypass 下山羊胎儿的血流分布 - 特别是关于动脉导管血流和胎儿人工器官。” 23. 921-924 (1994)

Sakata M,Hisano K,Okada M,Yasufuku M,Yokayama N,Uetani Y,Noma O,Nakamura H: "Experimental study of artificial placenta ; Doppler echocardiographic evaluation of goat fetal ductal blood velocity and waveforms in artificial placenta." Jpn J Artif Organs. 23

Sakata M，Hisano K，Okada M，Yasufuku M，Yokayama N，Uetani Y，Noma O，Nakamura H：“人工胎盘的实验研究；人工胎盘中山羊胎儿导管血流速度和波形的多普勒超声心动图评估。”