A study on pathophysiology of circulatory dysfunction in immature fetuses by extrauterine fetal incubation

宫外孵化未成熟胎儿循环功能障碍的病理生理学研究

• 批准号: 05454344
• 负责人: OKAI Takashi
• 金额: $ 3.9万
• 依托单位: UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454344/
• 关键词: artificial placenta; fetus; cerebral blood flow; colored microsphere; oxygen consumption; ECMO; ECMO; 胎児循環; 胎動抑制; 異常胎仔

1. The condition of isolated goat fetuses with umibilical arteriovenous extracorporeal membrane oxygenation was investigated. (1) Umbilical arteriovenous extracorporeal membrane oxygenation enabled isolated goat fetuses to survive for three weeks. (2) It was confirmed that fetal circulation was maintained with the patent ductus arteriosus during the extrauterine incubation period. (3) The fetuses maintained stable circlatory and respiratory paramaters after the postoperative period. (4) Oxygen consumption in the incubated fetuses was almost the same as the level in the intrauterine fetuses.2. Effects of repeated umbilical cord occlusion on brain circulation in fetal goats were investigated by colored microsphere technique. (1) The blood flow to the cerebral cortex, white matter, cerebellum and medulla increased by 200-300% respectively during umbilical cord occlusion. (2) The regional blood flow in the brain increased further after the release of cord occlusion. However, it tended to decrease with the repetition of cord occlusion.

1. 研究了山羊离体胎儿经脐动静脉体外膜氧合的情况。(1)脐动静脉体外膜氧合使离体山羊胎儿存活3周。(2)证实在子宫外潜伏期，动脉导管未闭维持了胎儿循环。(3)术后胎儿循环和呼吸参数保持稳定。(4)胎内耗氧量与胎内耗氧量基本一致。采用彩色微球技术研究了反复脐带阻断对胎山羊脑循环的影响。(1)脐带阻断时，大脑皮层、白质、小脑和髓质的血流量分别增加200 ~ 300%。(2)解除脊髓闭塞后，脑内局部血流量进一步增加。然而，随着脊髓闭塞的重复，它有减少的趋势。

项目成果

Unno N, Kuwabara Y, Okai T, et al: "Development of an artificial placenta : Endocrine responses of goat fetuses furing long-term extrauterine incubation with umbilical arteriovenons sxtracorpored membrane Oxygenatis" Eudocrine Journal. 41. S69-S76 (1994)

Unno N、Kuwabara Y、Okai T 等人：“人工胎盘的发育：长期宫外孵化与脐动脉体外膜氧合的山羊胎儿的内分泌反应”Eucocrine Journal。

N.Unnc,T.okai,et al.: "Development of an Artificial Placenta:Endocrine Responses of Goat Fetuses during Long-Term Extrauterine Incutation with Umbilical Arteriovenous Extracorporeal Membrane Oxygenation" Endocrine Journal. 41(Suppl). S69-S76 (1994)

N.Unnc、T.okai 等人：“人工胎盘的发育：采用脐动静脉体外膜氧合长期宫外切割过程中山羊胎儿的内分泌反应”内分泌杂志。

Nobuya Unno, Yoshinori Kuwabara, Takashi Okai, Hirotoshi Nakayama, Akihiko Kikuchi, Yumiko Narumiya, Shiro Kozuma, Yuji Taketani, Masanori Tamura: "Development of an artificail Placenta : Survival of isolated goat fetuses for three weeks with unbilical A-

Nobuya Unno、Yoshinori Kuwabara、Takashi Okai、Hirotoshi Nakayama、Akihiko Kikuchi、Yumiko Narumiya、Shiro Kozuma、Yuji Taketani、Masanori Tamura：“人工胎盘的开发：离体山羊胎儿在脐带 A 中存活三周

海野信也、岡井崇、他: "臍帯動静脈A・V ECMOによるヤギ未熟胎仔の長期間子宮外保育法-胎動抑制法の検討" 人工臓器. 22. 876-879 (1993)

Shinya Unno、Takashi Okai 等人：“使用脐带动静脉 A/V ECMO 进行早产山羊胎儿的长期宫外孵化 - 胎动抑制方法的研究”《人工器官》22. 876-879 (1993)。

Unno N,Kido K,Ryo E,Okai T,Kuwabara Y,Taketani Y.: "Long-term extrauterine incubation of premature goat fetuses using umbilical arteriovenous membrane oxygenation--The fetal immobilization method--" Japanese Journal of Artificial Organs. 22 (3). 876-879 (

Unno N，Kido K，Ryo E，Okai T，Kuwabara Y，Taketani Y.：“利用脐动静脉膜氧合技术对早产山羊胎儿进行长期宫外孵化——胎儿固定法——”日本人工器官杂志。