Development of Extrauterine Fetal Incubation System

宫外胎儿孵化系统的研制

• 批准号: 02557063
• 负责人: OKAI Takashi
• 金额: $ 11.46万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02557063/
• 关键词: Fetus; Artificial placenta; Extracorporeal Circulation; ECMO; Artificial amniotic fluid; Goat; 人工子宮; A-V ECMO; 子宮外胎児保育

Our previous study indicated that the oxygen consumption of an isolated goat fetus, incubated in an isothermic environment with umbilical A-V ECMO, is almost equal to that of fetuses in utero. However the exteriorized animals did not survive for more than 10 days. From observations of the fetal conditions during extrauterine fetal incubation (EUFI), fetal gross movement and water imbalance was strongly suspected as being the cause of circulatory deterioration during long-term incubation. We therefore hypothesized that if fetal movement were suppressed, long-term incubation of an immature fetus without circulatory deterioration would be possible. To test this hypothesis, we performed a series of EUFI experiments under a new protocol in which we administered a muscle relaxant to the fetus.From the results of the study, we have shown that total respiratory support with arteriovenous extracorporeal circulation is useful for the long-term incubation of premature goat fetuses in the EUFI system. Arteriovenous shunting has no fundamentally adverse effect on fetal survival. Maintenance of water homeostasis must be achieved for long-term incubation of isolated premature fetuses.

我们以前的研究表明，在等温环境中与脐A-V ECMO一起孵育的分离山羊胎儿的耗氧量几乎等于子宫内胎儿的耗氧量。然而，体外动物存活时间不超过10天。根据宫外胎仔孵育（EUFI）期间对胎仔状况的观察，强烈怀疑胎仔总体运动和水分失衡是长期孵育期间循环恶化的原因。因此，我们假设，如果胎动受到抑制，长期孵化未成熟的胎儿而不出现循环恶化是可能的。为了验证这一假设，我们进行了一系列的EUFI实验，根据一个新的协议，其中我们管理的肌肉松弛剂的fetus.From的研究结果，我们已经表明，总的呼吸支持与动静脉体外循环是有用的早产山羊胎儿在EUFI系统的长期孵化。动静脉分流术对胎儿存活没有根本性的不良影响。水平衡的维持必须实现长期孵化的孤立的早产儿。

项目成果

Nobuya Unno, Yoshinori kuwabara: "The difference between intrauterine and extrauterine enviroment-for the viewpoint of the development of artificial uterus." Perinatal Medicine. 22 (8). 1053-1056 (1992)

海野伸哉、桑原义德：“宫内与宫外环境的差异——从人工子宫的发展角度来看”。

Nobuya Unno, Yoshinori Kuwabara, Takashi Okai, Masahiko Mizuno: "Development of artificial uterus-present situation." BIO INDUSTRY. 7. 322-331 (1990)

Nobuya Unno、Yoshinori Kuwabara、Takashi Okai、Masahiko Mizuno：“人工子宫的发展-现状”。

Yoshinori Kuwabara: "Experimental perinatology-The possibility of extrauterine fetal incubation." Acta Obstetrica et Gynecologica Japonica. 44 (8). 982-986 (1992)

Yoshinori Kuwabara：“围产期实验——胎儿宫外孵化的可能性。”

Nobuya Unno,Yoshinori Kuwano,Norio Shinozuka,Kazuhiro Akiba,Takashi Okai,Shiro Kozuma,Masahiro Mizuno: "Development of avfificial placenta:Orygen metabolisum of isolated goat fetuses with umbilical avtevioーvenous extracorporeal membrane oxygenation" Fefal

Nobuya Unno，Yoshinori Kuwano，Norio Shinozuka，Kazuhiro Akiba，Takashi Okai，Shiro Kozuma，Masahiro Mizuno：“人工胎盘的发育：离体山羊胎儿的脐带静脉体外膜氧合的Orygen代谢”Fefal

海野信也、桑原慶紀: "実験治療の最前線 : 人工子宮--ヤギ胎仔子宮外保育実験の現況" 病態生理. 10. 702-708 (1991)

Shinya Unno，Yoshiki Kuwahara：“实验治疗的前沿：人工子宫——山羊胎儿宫外孵化实验的现状”病理生理学 10. 702-708 (1991)。