Hepatic reserve function assesed by integrated liver components : Mitochondrial, microsomal and reticuloendothelial system.

本项目旨在通过同时测量肝脏线粒体、微粒体和网状内皮系统三个区室的功能来评价肝脏储备功能。测定肝脏能量电荷（EC）和动脉酮体比（AKBR）作为肝脏线粒体的参数，测定血清7 α -羟胆固醇和胆固醇7 α -羟化酶（ch7α - oh）作为肝脏微粒体功能，测定纤维连接蛋白作为网状内皮系统功能。我们已经报道过这些系统评价具有临床重要性。在本研究中，我们研究了1)胆道梗阻后线粒体和微粒体功能与肝脏再生的关系，2)选择性门静脉结扎（PVL）和非结扎（PVNL）肝叶中肝脏能量状态与肝脏再生的关系，3)部分门静脉闭塞在扩大肝切除术前的优势。在胆道梗阻模型中，由于长期梗阻性黄疸进行性损伤肝细胞，提示胆道减压是必要的。在选择性门静脉闭塞模型中，再生需要EC，但DNA合成不仅仅由EC调节，因为EC的下降并不能预测随后的肝脏再生。本研究中AKBR未反映全肝EC。PVL、PVNL肝叶和假手术肝组织中ch7 α - oh活性无显著性差异。在实验3中，我们认为选择性门脉阻断在扩大肝切除术前具有优势，因为EC没有下降，肝脏再生恢复速度比不进行选择性门脉阻断的相应肝切除术的动物更快。

The aim of the project is to evaluate hepatic reserve function by measuring the function of three compartments namely, liver mitochondria, microsome and reticuloendothelial system simultaneously. Energy charge (EC) of the liver and arterial ketone body ratio (AKBR) were measured as the parameter of the liver mitochondria, serum 7alpha-hydroxycholesterol and cholesterol 7alpha-hydroxylase (CH7alpha-OH) as the liver microsomal function, and fibronectin as the reticuloendothelial systemic function. We have reported that these systemic evaluation is of clinical importance. In the present study, we examined 1) mitochondrial and microsomal functions and liver regeneration following biliary obstruction, 2) the relationship between hepatic energy status and liver regeneration in the selective portal vein ligated (PVL) and nonligated (PVNL) lobes of the liver, 3) advantages of partial portal occlusion preceding extended hepatectomy. In the model of biliary obstruction, it is suggested that biliary decompression is neccessary, since the prolonged obstructive jaundice progressively injured hepatocyte. In the selective portal vein occlusion model, EC was required for regeneration but DNA synthesis was not merely modulated by EC because decline in EC did not predict the following liver regeneration. AKBR did not reflect EC of the whole liver in this study. The microsomal function was preserved and there was no significant difference in CH 7alpha-OH activity among PVL,PVNL lobes and sham-operated liver. In the experiment 3, it was suggested that selective portal occlusion prior to extended hepatectomy had advantage because EC did not decline and liver regeneration restored faster than animals underwent corresponding hepatectomy without selective portal occlusion.