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Hepatic reserve function assesed by integrated liver components : Mitochondrial, microsomal and reticuloendothelial system.

通过综合肝脏成分评估肝脏储备功能：线粒体、微粒体和网状内皮系统。

基本信息

批准号：
05671071
负责人：
CHIJIIWA Kazuo
金额：
$ 1.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The aim of the project is to evaluate hepatic reserve function by measuring the function of three compartments namely, liver mitochondria, microsome and reticuloendothelial system simultaneously. Energy charge (EC) of the liver and arterial ketone body ratio (AKBR) were measured as the parameter of the liver mitochondria, serum 7alpha-hydroxycholesterol and cholesterol 7alpha-hydroxylase (CH7alpha-OH) as the liver microsomal function, and fibronectin as the reticuloendothelial systemic function. We have reported that these systemic evaluation is of clinical importance. In the present study, we examined 1) mitochondrial and microsomal functions and liver regeneration following biliary obstruction, 2) the relationship between hepatic energy status and liver regeneration in the selective portal vein ligated (PVL) and nonligated (PVNL) lobes of the liver, 3) advantages of partial portal occlusion preceding extended hepatectomy. In the model of biliary obstruction, it is suggested that biliary decompression is neccessary, since the prolonged obstructive jaundice progressively injured hepatocyte. In the selective portal vein occlusion model, EC was required for regeneration but DNA synthesis was not merely modulated by EC because decline in EC did not predict the following liver regeneration. AKBR did not reflect EC of the whole liver in this study. The microsomal function was preserved and there was no significant difference in CH 7alpha-OH activity among PVL,PVNL lobes and sham-operated liver. In the experiment 3, it was suggested that selective portal occlusion prior to extended hepatectomy had advantage because EC did not decline and liver regeneration restored faster than animals underwent corresponding hepatectomy without selective portal occlusion.

该项目的目的是通过同时测量肝脏线粒体、微粒体和网状内皮系统三个部分的功能来评估肝脏储备功能。测定肝脏的能荷（EC）和动脉酮体比率（AKBR）作为肝脏线粒体的参数，血清7 α-羟基胆固醇和胆固醇7 α-羟化酶（CH 7 α-OH）作为肝脏微粒体功能，纤连蛋白作为网状内皮系统功能。我们已经报道了这些系统评价具有临床重要性。在本研究中，我们研究了1）线粒体和微粒体功能和肝再生后胆道梗阻，2）之间的关系，肝能量状态和肝再生的选择性门静脉结扎（PVL）和非结扎（PVNL）的肝叶，3）扩大肝切除术前的门静脉部分阻断的优势。在胆道梗阻模型中，胆道减压是必要的，因为梗阻性黄疸的持续进行性损伤肝细胞。在选择性门静脉阻断模型中，EC是再生所必需的，但DNA合成不仅由EC调节，因为EC下降并不能预测随后的肝再生。AKBR在本研究中未反映整个肝脏的EC。微粒体功能得以保留，PVL、PVNL叶和假手术肝之间CH 7 α-OH活性无显著差异。在实验3中，有人建议，扩大肝切除术前的选择性门静脉阻断有优势，因为EC没有下降，肝再生恢复快于动物接受相应的肝切除术没有选择性门静脉阻断。