The total evaluation of the functions of microsome, mitochondria, and reticuloendothelial systems in the liver

(1)术前胆道内引流对梗阻性黄疸大鼠肝切除术后肝再生和功能的作用优于胆道外引流上级。我们研究了梗阻性黄疸大鼠术前胆道内外引流后肝切除术后肝脏再生率和功能的差异。研究方法：将雄性Wistar大鼠随机分为梗阻性黄疸肝切除组（OJ-Hx）、胆道外引流肝切除组（ED-Hx）和胆道内引流肝切除组（ID-Hx）。评估肝脏再生、能量状态和肝功能。结果：ID-Hx组大鼠肝相对重量和DNA合成率明显高于其他两组。肝组织丙二醛和血清透明质酸含量 关于我们 ID-Hx组的摄食量最低。两种胆道引流术均降低了门静脉内毒素浓度。3组间肝脏三磷酸腺嘌呤浓度和能荷水平相似。结论：虽然肝切除术前胆道内外引流均能改善肝功能，但胆道内引流上级外引流，肝切除术后肝再生和功能更好。(Ref. 3）（2）钆预处理降低大鼠缺血/再灌注下部分肝切除术后的存活率并损害肝再生。通过用氯化钆（Gd）处理来调节枯否细胞功能可保护肝脏免受再灌注损伤。使用缺血/再灌注技术临床使用，我们研究了Gd对大鼠肝切除术后肝再生的影响。方法. Gd预处理组（Gd组）和生理盐水预处理组（对照组）大鼠在缺血15 min再灌注15 min后，于缺血15 min行70%肝切除。评估肝脏再生、能量状态和肝功能。结果Gd组的24小时存活率（67%）明显低于对照组（100%）。术后第1天，Gd组大鼠肝脏相对重量和DNA合成率显著低于对照组。术后第1天，Gd组血清总胆红素和内毒素水平显著高于对照组。肝切除术后即刻，Gd组的肝脏ATP水平显著低于对照组。结论在缺血/再灌注条件下，Gd预处理损害肝切除术后的肝再生和能量状态，并降低术后存活率。(Ref. 6）减

(1) Preoperative internal biliary drainage is superior to external biliary drainage in liver regeneration and function after hepatectomy in obstructive jaundiced ratsBackgrounds : The significance of biliary drainage before surgery is controversial in patients with obstructive jaundice. We examined the differences in regeneration rates and function of the liver after hepatectomy in obstructive jaundiced rats with preoperative external and internal biliary drainage. Methods : After biliary obstruction for 7 days, Male Wistar rats were randomly divided into 3 groups : obstructive jaundice and hepatectomy (OJ-Hx), external biliary drainage and hepatectomy (ED-Hx), and internal biliary drainage and hepatectomy (ID-Hx). The liver regeneration, energy status, and liver functions were evaluated. Results : The relative liver weight and DNA synthesis rate were significantly higher than in the ID-Hx group than in the other two groups. The hepatic malondialdehyde and serum hyaluronic acid concent … More rations were lowest in the ID-Hx group. Both biliary drainage procedures lowered the portal endotoxin concentration. Hepatic adenine triphosphate concentrations and energy charge levels were similar among the 3 groups. Conclusions : Although both external and internal biliary drainage before hepatectomy improved the liver function, internal biliary drainage was superior to external drainage, as evidenced by the better liver regeneration and function after hepatectomy. (Ref. 3)(2) Gadolinium pretreatment decreased survival and impairs liver regeneration after partial hepatectomy under ischemia/repercussion in ratsBackground. Modulation of Kupffer cell functions by treatment with gadolinium chloride (Gd) protects the liver against reperfusion injury. Using a ischemia/reperfusion technique clinically used, we examined the effect of Gd on liver regeneration after hepatectomy in rats. Methods. After an initial 15-minute ischemia and 15-minute reperfusion, 70% hepatectomy was performed during the second 15-minute ischemia period in Gd pretreated (Gd group) and saline pretreated (control group) rats. The liver regeneration, energy status, and liver functions were evaluated. Results. The 24-hour survival rate was significantly lower in the Gd group (67%) than in the control group (100%). On POD1, the relative liver weight and DNA synthesis rate were significantly lower in the Gd group than in the control group. On POD1, serum total bilirubin and endotoxin levels were significantly higher in the Gd group than in the control group. Immediately after hepatectomy, the hepatic ATP level was significantly lower in the Gd group than in the control group. Conclusion. Under ischemia/reperfusion, Gd pretreatment impairs liver regeneration and energy status after hepatectomy, and decreases postoperative survival. (Ref. 6) Less