The total evaluation of the functions of microsome, mitochondria, and reticuloendothelial systems in the liver

(1)术前胆道内引流在梗阻性黄疸大鼠肝切除后肝脏再生和功能方面优于胆管外引流。我们研究了梗阻性黄疸大鼠肝切除术后，胆道外引流和胆道内引流对肝再生率和功能的影响。方法：雄性Wistar大鼠胆道梗阻7d后，随机分为梗阻性黄疸肝切除(OJ-HX)组、胆管外引流肝切除(ED-HX)组和胆总管内引流肝切除(ID-HX)组。对肝脏再生、能量状态和肝功能进行评估。结果：ID-HX组小鼠肝脏相对重量和DNA合成率明显高于其他两组。肝丙二醛和血清透明质酸浓度…ID-HX组中更多的配给是最低的。两种胆道引流方法均可降低门静脉内毒素浓度。三组大鼠肝脏三磷酸腺苷浓度和能荷水平相似。结论：尽管肝切除术前胆道外引流和胆道内引流均能改善肝功能，但内引流优于外引流，肝切除后肝再生和功能恢复良好。(参考文献)(3)(2)在大鼠肝部分切除缺血/再灌流模型中，Gd预处理使大鼠存活率降低，肝再生受损。氯化钆(Gd)对Kupffer细胞功能的调节保护肝脏免受再灌注损伤。采用临床常用的缺血再灌流技术，观察了Gd对大鼠肝切除后肝再生的影响。方法：研究方法。Gd预处理组(Gd组)和生理盐水组(对照组)在初始缺血15min再灌流15min后，在第二个15min缺血期行70%的肝切除。对肝脏再生、能量状态和肝功能进行评估。结果。Gd组的24小时存活率(67%)明显低于对照组(100%)。在POD1点，Gd组小鼠的相对肝脏重量和DNA合成率显著低于对照组。在POD1时，Gd组血清总胆红素和内毒素水平显著高于对照组。肝切除后即刻，Gd组大鼠肝脏ATP水平明显低于对照组。结论。在缺血/再灌流条件下，Gd预处理损害了肝切除后的肝脏再生和能量状态，降低了术后存活率。(参考文献)6)更少

(1) Preoperative internal biliary drainage is superior to external biliary drainage in liver regeneration and function after hepatectomy in obstructive jaundiced ratsBackgrounds : The significance of biliary drainage before surgery is controversial in patients with obstructive jaundice. We examined the differences in regeneration rates and function of the liver after hepatectomy in obstructive jaundiced rats with preoperative external and internal biliary drainage. Methods : After biliary obstruction for 7 days, Male Wistar rats were randomly divided into 3 groups : obstructive jaundice and hepatectomy (OJ-Hx), external biliary drainage and hepatectomy (ED-Hx), and internal biliary drainage and hepatectomy (ID-Hx). The liver regeneration, energy status, and liver functions were evaluated. Results : The relative liver weight and DNA synthesis rate were significantly higher than in the ID-Hx group than in the other two groups. The hepatic malondialdehyde and serum hyaluronic acid concent … More rations were lowest in the ID-Hx group. Both biliary drainage procedures lowered the portal endotoxin concentration. Hepatic adenine triphosphate concentrations and energy charge levels were similar among the 3 groups. Conclusions : Although both external and internal biliary drainage before hepatectomy improved the liver function, internal biliary drainage was superior to external drainage, as evidenced by the better liver regeneration and function after hepatectomy. (Ref. 3)(2) Gadolinium pretreatment decreased survival and impairs liver regeneration after partial hepatectomy under ischemia/repercussion in ratsBackground. Modulation of Kupffer cell functions by treatment with gadolinium chloride (Gd) protects the liver against reperfusion injury. Using a ischemia/reperfusion technique clinically used, we examined the effect of Gd on liver regeneration after hepatectomy in rats. Methods. After an initial 15-minute ischemia and 15-minute reperfusion, 70% hepatectomy was performed during the second 15-minute ischemia period in Gd pretreated (Gd group) and saline pretreated (control group) rats. The liver regeneration, energy status, and liver functions were evaluated. Results. The 24-hour survival rate was significantly lower in the Gd group (67%) than in the control group (100%). On POD1, the relative liver weight and DNA synthesis rate were significantly lower in the Gd group than in the control group. On POD1, serum total bilirubin and endotoxin levels were significantly higher in the Gd group than in the control group. Immediately after hepatectomy, the hepatic ATP level was significantly lower in the Gd group than in the control group. Conclusion. Under ischemia/reperfusion, Gd pretreatment impairs liver regeneration and energy status after hepatectomy, and decreases postoperative survival. (Ref. 6) Less