The total evaluation of the functions of microsome, mitochondria, and reticuloendothelial systems in the liver

肝脏微粒体、线粒体、网状内皮系统功能的综合评价

• 批准号: 09671318
• 负责人: CHIJIIWA Kazuo
• 金额: $ 1.98万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671318/
• 关键词: obstructive jaundice; biliary drainage; hepatectomy; gadolinium; ischemia; reperfusion injury; Kupffer細胞; 肝切除; 肝再生; 肝予備能; 他臓器合併肝切除

(1) Preoperative internal biliary drainage is superior to external biliary drainage in liver regeneration and function after hepatectomy in obstructive jaundiced ratsBackgrounds : The significance of biliary drainage before surgery is controversial in patients with obstructive jaundice. We examined the differences in regeneration rates and function of the liver after hepatectomy in obstructive jaundiced rats with preoperative external and internal biliary drainage. Methods : After biliary obstruction for 7 days, Male Wistar rats were randomly divided into 3 groups : obstructive jaundice and hepatectomy (OJ-Hx), external biliary drainage and hepatectomy (ED-Hx), and internal biliary drainage and hepatectomy (ID-Hx). The liver regeneration, energy status, and liver functions were evaluated. Results : The relative liver weight and DNA synthesis rate were significantly higher than in the ID-Hx group than in the other two groups. The hepatic malondialdehyde and serum hyaluronic acid concent … More rations were lowest in the ID-Hx group. Both biliary drainage procedures lowered the portal endotoxin concentration. Hepatic adenine triphosphate concentrations and energy charge levels were similar among the 3 groups. Conclusions : Although both external and internal biliary drainage before hepatectomy improved the liver function, internal biliary drainage was superior to external drainage, as evidenced by the better liver regeneration and function after hepatectomy. (Ref. 3)(2) Gadolinium pretreatment decreased survival and impairs liver regeneration after partial hepatectomy under ischemia/repercussion in ratsBackground. Modulation of Kupffer cell functions by treatment with gadolinium chloride (Gd) protects the liver against reperfusion injury. Using a ischemia/reperfusion technique clinically used, we examined the effect of Gd on liver regeneration after hepatectomy in rats. Methods. After an initial 15-minute ischemia and 15-minute reperfusion, 70% hepatectomy was performed during the second 15-minute ischemia period in Gd pretreated (Gd group) and saline pretreated (control group) rats. The liver regeneration, energy status, and liver functions were evaluated. Results. The 24-hour survival rate was significantly lower in the Gd group (67%) than in the control group (100%). On POD1, the relative liver weight and DNA synthesis rate were significantly lower in the Gd group than in the control group. On POD1, serum total bilirubin and endotoxin levels were significantly higher in the Gd group than in the control group. Immediately after hepatectomy, the hepatic ATP level was significantly lower in the Gd group than in the control group. Conclusion. Under ischemia/reperfusion, Gd pretreatment impairs liver regeneration and energy status after hepatectomy, and decreases postoperative survival. (Ref. 6) Less

(1)对于梗阻性黄疸大鼠肝切除术后的肝再生和功能，术前胆汁内引流优于胆汁外引流背景：梗阻性黄疸患者术前胆汁引流的意义存在争议。我们检查了梗阻性黄疸大鼠肝切除术后肝再生率和功能的差异，并进行了术前外胆管引流和内胆管引流。方法：胆道梗阻7 d后，雄性Wistar大鼠随机分为3组：梗阻性黄疸肝切除组（OJ-Hx）、胆道外引流肝切除组（ED-Hx）、胆道内引流肝切除组（ID-Hx）。评估肝再生、能量状态和肝功能。结果：ID-Hx组的相对肝脏重量和DNA合成率显着高于其他两组。 ID-Hx 组的肝脏丙二醛和血清透明质酸浓度最低。两种胆道引流手术均降低了门脉内毒素浓度。三组之间的肝三磷酸腺嘌呤浓度和能量电荷水平相似。结论：虽然肝切除术前胆道外引流和胆道内引流均改善了肝功能，但胆道内引流优于胆道外引流，肝切除后肝再生和功能更好就证明了这一点。 （参考文献 3）(2) 钆预处理会降低大鼠局部肝切除术后局部缺血/反应的存活率并损害肝再生背景。通过氯化钆 (Gd) 治疗调节库普弗细胞功能可保护肝脏免受再灌注损伤。我们利用临床上使用的缺血/再灌注技术，研究了Gd对大鼠肝切除术后肝再生的影响。方法。在最初的 15 分钟缺血和 15 分钟再灌注后，在 Gd 预处理（Gd 组）和盐水预处理（对照组）大鼠的第二个 15 分钟缺血期间进行 70% 肝切除术。评估肝再生、能量状态和肝功能。结果。 Gd组的24小时生存率（67%）显着低于对照组（100%）。在 POD1 时，Gd 组的相对肝脏重量和 DNA 合成率显着低于对照组。 POD1时，Gd组的血清总胆红素和内毒素水平显着高于对照组。肝切除后立即，Gd 组的肝脏 ATP 水平显着低于对照组。结论。在缺血/再灌注条件下，Gd预处理会损害肝切除术后的肝再生和能量状态，并降低术后生存率。 （参考文献 6） 减

项目成果

Chijiwa K, Watanabe M: "Serum Hyaluronic Acid Level Reflects Volume and ATP Levels of the Liver after Extended Hepatectomy with and without Preoperative Portal Vein Occlusion"J Surg Res. 72. 107-111 (1997)

Chijiwa K，Watanabe M：“血清透明质酸水平反映了有或没有术前门静脉闭塞的扩大肝切除术后肝脏的体积和 ATP 水平”J Surg Res。

Chijiwa K, Saiki S: "Effect of Preoperative Portal Vein Embolization on Liver Volume and Hepatic Energy Status of the Nonembolized Liver Lobe in Humans"European Surgical Research. (in press).

Chijiwa K、Saiki S：“术前门静脉栓塞对人类非栓塞肝叶的肝脏体积和肝能量状态的影响”欧洲外科研究。

Saiki S, Chijiwa K: "Preoperative Internal Biliary Drainage Is Superior to External Biliary Drainage in Live Regeneration and Function After Hepatectomy in Obstructive Jaundiced Rats"Annals of Surgery. 230(5). 655-662 (1999)

Saiki S、Chijiwa K：“在梗阻性黄疸大鼠肝切除术后的活体再生和功能方面，术前胆汁内引流优于胆汁外引流”《外科年鉴》。

Watanabe M, Chijiwa K: "Effects of Streptococcal Preparation OK-432 on Liver Regeneration and Energy Status After Partial Hepatectomy under Ischemia/Reperfusion in Rats"Hepato-Gastroenterology. (in press).

Watanabe M、Chijiwa K：“链球菌制剂 OK-432 对大鼠缺血/再灌注下部分肝切除术后肝脏再生和能量状态的影响”肝胃肠病学。

Watanabe M, Chijiwa K: "Gadolinum Pretreatment Decreases Survival and Impairs Liver Regeneration After Partial Hepatectomy Under Ischemia/Reperfusion in Rats"Surgery. (in press).

Watanabe M、Chijiwa K：“缺血/再灌注大鼠部分肝切除术后钆预处理会降低存活率并损害肝脏再生”手术。