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The distribution and monomer activity of cholesterol in micellar solution - its significance in gallstone formation -

胶束溶液中胆固醇的分布和单体活性-其在胆结石形成中的意义-

基本信息

批准号：
62570609
负责人：
CHIJIIWA Kazuo
金额：
$ 1.28万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The differences in cholesterol distribution, cholesterol monomer activity and the concentrations of biliary lipids and protein related with nucleation time between the gallbladder biles with and without gallstone were examined to clarify the etiology of cholesterol gallstone formation. Gallbladder biles were obtained by the direct needle puncture at surgery from two groups of patients with cholelithisasis or with diseases other than hepatobiliary disease. The filtered biles through the 0.22 um membrane to remove cholesterol crystals had similar concentrations of biliary lipids and biliary proteins. Cholesterol monomer activity was not significantly different between two groups. nucleation time was significantly faster in gallstone patients. The filtrate through the XM-300 membrane had a similar nucleation time due to the prolonged nucleation time in gallstone group. This filtration retained more cholesterol, phospholipid and biliary protein in gallstone patients, while the filtration rate of bile salt was similar between two groups. These results suggest that the protein which cannot pass through the XM-300 membrane but not the total protein is responsible for cholesterol monohydrate crystallization. The removal of vesicles mainly composed of cholesterol and phospholipid prolongs the nucleation time. Cholesterol monomer activity as well as cholesterol saturation index cannot distinguish cholesterol gallstone disease from control.

研究有胆结石和无胆结石的胆囊胆汁中胆固醇分布、胆固醇单体活性以及与成核时间相关的胆汁脂质和蛋白质浓度的差异，以阐明胆固醇胆结石形成的病因。两组胆石症或肝胆疾病以外疾病患者通过手术直接针刺获取胆囊胆汁。通过 0.22 um 膜过滤去除胆固醇晶体的胆汁具有相似浓度的胆汁脂质和胆汁蛋白质。两组之间的胆固醇单体活性没有显着差异。胆结石患者的成核时间明显更快。由于胆石组成核时间延长，通过 XM-300 膜的滤液具有相似的成核时间。这种过滤在胆结石患者中保留了更多的胆固醇、磷脂和胆汁蛋白，而两组之间胆汁盐的过滤率相似。这些结果表明，不能通过 XM-300 膜的蛋白质而非总蛋白质负责一水胆固醇结晶。去除主要由胆固醇和磷脂组成的囊泡延长了成核时间。胆固醇单体活性以及胆固醇饱和指数不能区分胆固醇胆结石疾病和对照。