BASIC STUDY FOR SELECTIVE GENE TARGETING THERAPY OF COLON CANCER USING MONOCLONAL ANTIBODY

1993年，我们进行了用单抗结合的反义DNA选择性免疫治疗结肠癌的试验，但结果并不理想。1994年，反义DNA导入逆转录病毒包被抗结肠癌单抗的逆转录病毒，感染人结肠癌细胞株，c-myc基因的表达明显增强。c-myc基因的表达在抗原阳性的癌细胞中受到抑制，而在无抗原的癌细胞中不表达。单抗的大小改变了反义DNA的亲和力，导致反义DNA在靶细胞中的表达降低。

IN 1993, THE TRIAL TO APPLY MONOCLONAL ANTIBODY BOUND ANTISENSE DNA FOR SELECTIVE IMMUNOTARGETING GENE THERAPY OF COLON CANCER HAD BEEN CONDUCTED, THOUGH THE RESULTS WERE NOT SATISFACTORY.IN 1994, ANTISENSE DNA INTORDUCED INTO RETRO-VIRUS THOSE WERE COATED WITH MONOCLONAL ANITOBODY AGAINST COLON CANCER HAD BEEN DEVELOPED.THE HUMAN COLON CANCER CELL LINES WERE INFECTED AND THE EXPRESSION OF c-myc GENE WAS EXAMINED.THE EXPRESSION OF c-myc GENE WAS INHIBITED IN ANTIGEN POSITIVE CANCER CELL LINES BUT NOT IN CELL LINES WITHOUT ANTIGEN.THE SIZE OF MONOCLONAL ANTIBODY CHANGED THE AFFINITY OF ANTIBODEY RESULTED IN DECREASED EXPRESSION OF ANTISENSE DNA IN TARGET CELLS.TEH MOST IMPORTANT PROBRLEM TO OVERCOME SEEMED POOR EFFEICENCY OF VIRUS INFECTION.