Influence of salinity on novel (moderately) thermophilic nitrite-oxidizing bacteria
盐度对新型(中度)嗜热亚硝酸氧化细菌的影响
基本信息
- 批准号:432963485
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:
- 资助国家:德国
- 起止时间:
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Nitrification, the biological oxidation of ammonia to nitrite and further to nitrate, is of environmental importance to avoid accumulation of harmful ammonia and nitrite, which can result in human and aquatic animal health risk. In WWTPs its functioning has to be maintained even under adverse conditions caused for example by elevated temperature or salinity. Especially the fastidious nitrite-oxidizing bacteria (NOB) that perform the second step of nitrification are not sufficiently investigated. From geothermal systems and warm sewage, Nitrospira and different Chloroflexi NOB were already cultivated, but metagenome data indicated the presence of a highly diverse community of putative NOB by specific sequences of the key enzyme, the nitrite oxidoreductase (NXR). In the first phase of this project, directed cultivation approaches led to the description of new (moderately) thermophilic species of Nitrospira and Nitrolancea. Additionally, several novel NOB candidates growing at elevated temperature (37-50°C) from diverse phyla (Ca. Dadabacteria, Actinobacteria, Chloroflexi, Acidobacteria, (Delta)-Proteobacteria, Nitrospirae) were selectively enriched and correlated with specific nxrA/B and 16S rRNA gene sequences as well as distinct morphotypes, but their 16S rRNA gene similarities to the next taxonomically described bacteria are rather low (80-94%). Molecular characterization of metagenome assembled genomes (MAGs) gave first insights into their carbon, nitrogen and oxygen metabolism including the finding of rubisco genes for some Chloroflexi and Actinobacteria, supporting their chemolithoautotrophic lifestyle and differentiating them from denitrifiers. In the past, new NOB isolates were obtained by various selecting factors (nitrite concentration, temperature, DO, pH), whereas in the second part of the project, salinity stress (especially nitrate) will be the main driver for niche separation in combination with high temperature. Preliminary results indicated high NaCl tolerances of Dehalococcoides-like NOB and novel Actinobacteria and reduced abundance of Nitrospira. It is the goal to highly enrich/isolate novel salt- and heat-tolerant NOB by cell sorting, to characterize them physiologically and to screen metagenomes for new metabolic pathways. RNA based gene expression and metatranscriptome data obtained under different growth conditions will verify activity of novel nxr genes and give insights into tolerance mechanisms. Most promising candidates will be tested for their ability to grow in bioreactor systems under salt and temperature stress for biotechnological applications. With regard to a warming world, there is a growing interest in nitrification under thermal conditions and it has to be ensured that agricultural nitrification inhibitors will still affect a shifting community of nitrifying microorganisms. Furthermore, thermophilic bacteria as survivors of early Earth are interesting with regard to evolutionary aspects.
硝化作用是氨生物氧化为亚硝酸盐并进一步氧化为硝酸盐,对于避免有害氨和亚硝酸盐的积累具有重要的环境意义,而有害氨和亚硝酸盐的积累会导致人类和水生动物的健康风险。在污水处理厂中,即使在温度或盐度升高等不利条件下,也必须保持其功能。特别是进行硝化第二步的难养亚硝酸盐氧化细菌(NOB)没有得到充分的研究。从地热系统和温暖的污水中,硝化螺旋菌和不同的Chloroflexi NOB已经被培养出来,但宏基因组数据表明,通过关键酶亚硝酸盐氧化还原酶(NXR)的特定序列,存在高度多样性的推定NOB群落。在该项目的第一阶段,定向培养方法导致描述了Nitrospira和Nitrolancea的新(中度)嗜热物种。此外,几个新的NOB候选人生长在高温(37-50°C)从不同的门(Ca. Dadabacteria、Actinobacteria、Chloroflexi、Acidobacteria、(Delta)-Proteobacteria、Nitrosspirit)被选择性富集并与特定的nxrA/B和16 S rRNA基因序列以及不同的形态型相关,但它们与下一个分类学描述的细菌的16 S rRNA基因相似性相当低(80-94%)。宏基因组组装基因组(MAG)的分子特征首次揭示了它们的碳、氮和氧代谢,包括发现一些绿球藻和放线菌的rubisco基因,支持它们的化能无机自养生活方式,并将它们与反硝化菌区分开来。过去,新的NOB分离株是通过各种选择因素(亚硝酸盐浓度、温度、DO、pH)获得的,而在该项目的第二部分,盐胁迫(尤其是硝酸盐)将成为生态位分离的主要驱动因素,并结合高温。初步结果表明,高NaCl耐受性的Dehalocococcoides样NOB和新的放线菌和减少丰度的Nitrospira。其目标是通过细胞分选高度富集/分离新型耐盐和耐热NOB,以生理学表征它们并筛选宏基因组用于新的代谢途径。在不同生长条件下获得的基于RNA的基因表达和元转录组数据将验证新型nxr基因的活性,并深入了解耐受机制。最有前途的候选人将测试他们的能力,在生物技术应用的盐和温度压力下的生物反应器系统中生长。关于变暖的世界,人们对热条件下的硝化作用越来越感兴趣,并且必须确保农业硝化抑制剂仍然会影响硝化微生物的移动群落。此外,嗜热细菌作为早期地球的幸存者在进化方面是有趣的。
项目成果
期刊论文数量(0)
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Privatdozentin Dr. Eva Spieck其他文献
Privatdozentin Dr. Eva Spieck的其他文献
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{{ truncateString('Privatdozentin Dr. Eva Spieck', 18)}}的其他基金
A renewed view on the complex community of nitrite oxidizing bacteria derived from sewage
对污水中亚硝酸盐氧化细菌复杂群落的新认识
- 批准号:
230977559 - 财政年份:2012
- 资助金额:
-- - 项目类别:
Research Grants
springsDiversität thermophiler nitrifizierender Gemeinschaften aus heißen Quellen
温泉中嗜热硝化群落的多样性
- 批准号:
133181405 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Research Grants
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