权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Chemosensitivity of gynecologic malignancies revealed by disease oriented screening system.

疾病导向筛查系统揭示妇科恶性肿瘤的化学敏感性。

基本信息

批准号：
05671352
负责人：
NISHIDA Masato
金额：
$ 1.34万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671352/
关键词：
Endometrial cancer Ovarian cancer Chemotherapy Chemosensitivity test Cell line DOSS 癌細胞株 DOSS

项目摘要

Studies are undertaken to examine the chemosensitivity of endometrial cancer and ovarian serous adenocarcinoma in vitro by MTT assay.Five kinds of established human endometrial cancer cell line, Ishikawa, HEC-1, HEC-50B,HEC-59 and HEC-88 and 3 kinds of established ovarian serous adenocarcinoma cell line, Yoshizaki, Asano and SHIN-3 were used in this study.Twenty-three kinds of antitumor agents including ADM,THP-ADM,EPI,ACR,DM,Act-D,BLM,PEP,NCS,MMC,VLB,VCR,VDS,Ara-C,5-FU,MTX,CPM,CDDP,CBDCA,254-S,MCNU,L-ASP,ETP were examined.The cells were inoculated into microplate at 1x10^4/well (day 0) , cultured for 3 days and exposed to each drug at 0,0.01,0.05,0.1,0.2,0.5,1,2,5,10 mug/ml, for 1,3,6,12,24,48,72hs (day 3) , washed free drug and incubate media alone. The enzyme activities were measured by MTT assay (day 7). The antitumor effects of each drug were evaluated from the surviving fraction curves.As a result, there was a difference in drug sensitivity between well differentiated type and poorly differentiated type in endometrial cancer.And this study revealed that ADM,THP-ADM,ACR,Act-D,NCS,VLB,CDDP,254-S and ETP were effective for endometrial cancer and ACR,Act-D and CDDP were effective for ovarian serous adenocarcinoma in vitro.

采用MTT法体外检测子宫内膜癌和卵巢浆液性腺癌的化疗敏感性。建立了5种已建立的人子宫内膜癌细胞系Ishikawa、HEC-1、HEC-50B、HEC-59和HEC-88以及3种已建立的卵巢浆液性腺癌细胞系Yoshizaki、Asano和本研究采用SHIN-3，检测ADM、THP-ADM、EPI、ACR、DM、Act-D、BLM、PEP、NCS、MMC、VLB、VCR、VDS、Ara-C、5-FU、MTX、CPM、CDDP、CBDCA、254-S、MCNU、L-ASP、ETP等23种抗肿瘤药物，接种细胞。以 1x10^4/孔（第 0 天）放入微孔板中，培养 3 天，并以 0,0.01,0.05,0.1,0.2,0.5,1,2,5,10 mug/ml 的浓度暴露于每种药物，持续 1,3,6,12,24,48,72 小时（第 3 天），洗涤游离药物并单独孵育培养基。通过MTT测定（第7天）测量酶活性。从存活分数曲线评价各药物的抗肿瘤作用，结果显示，子宫内膜癌中高分化型和低分化型的药物敏感性存在差异。本研究发现ADM、THP-ADM、ACR、Act-D、NCS、VLB、CDDP、254-S和ETP对子宫内膜癌有效，ACR、Act-D和CDDP对卵巢浆液性癌有效。体外腺癌。