Chemosensitivity of gynecologic malignancies revealed by disease oriented screening system.

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Studies are undertaken to examine the chemosensitivity of endometrial cancer and ovarian serous adenocarcinoma in vitro by MTT assay.Five kinds of established human endometrial cancer cell line, Ishikawa, HEC-1, HEC-50B,HEC-59 and HEC-88 and 3 kinds of established ovarian serous adenocarcinoma cell line, Yoshizaki, Asano and SHIN-3 were used in this study.Twenty-three kinds of antitumor agents including ADM,THP-ADM,EPI,ACR,DM,Act-D,BLM,PEP,NCS,MMC,VLB,VCR,VDS,Ara-C,5-FU,MTX,CPM,CDDP,CBDCA,254-S,MCNU,L-ASP,ETP were examined.The cells were inoculated into microplate at 1x10^4/well (day 0) , cultured for 3 days and exposed to each drug at 0,0.01,0.05,0.1,0.2,0.5,1,2,5,10 mug/ml, for 1,3,6,12,24,48,72hs (day 3) , washed free drug and incubate media alone. The enzyme activities were measured by MTT assay (day 7). The antitumor effects of each drug were evaluated from the surviving fraction curves.As a result, there was a difference in drug sensitivity between well differentiated type and poorly differentiated type in endometrial cancer.And this study revealed that ADM,THP-ADM,ACR,Act-D,NCS,VLB,CDDP,254-S and ETP were effective for endometrial cancer and ACR,Act-D and CDDP were effective for ovarian serous adenocarcinoma in vitro.