Mechanisms of gestagen therapy on endometrial cancer.

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An attempt was made to clarify the mechanisms of gestagen therapy on endometrial cancer or breast cancer.Mammary tumor was. induced in female Sprague-Dawley rats by oral administration of 7, 12-dimethybenz[a]anthracene(DMBA). Histologically, the induced mammary tumors were divided into 7 types such as well differentiated adenocarcinoma, moderately differentiated adenocarcinoma and so on. Estrogen receptor(ER)and progesterone receptor(PR)were positively determined in 43(87.8%)and 34(69.4%), respectively, out of 49 samples by the dextran-coated charcoal(DCC)method. A portion of DMBA-induced mammary tumor was transplanted subcutaneously into nude mice in 38 samples. All but one sample which contains neither ER nor PR, could not make tumors. The tumors which were autoimplanted into subcutaneous tissue of the rats did not develop.The primary culture of the tumor have been carried out in 35 times. Though the excellent cell growth of the epithelial cells and fibroblastic cells were seen at the primary stage, no cell lines have been established, because the cell numbers had been tapering at each subculture. We investigated the effects of medroxyprogesterone acetate(MPA)on the tumor developed in nude mice. No apparent antitumor effects were seen. We also investigated antitumor effects of MPA in vitro using the cell strain. There were no difference between the MPA group and the control group.