Mechanisms of gestagen therapy on endometrial cancer.

孕激素治疗子宫内膜癌的机制。

• 批准号: 01570916
• 负责人: NISHIDA Masato
• 金额: $ 1.41万
• 依托单位: Tsukuba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570916/
• 关键词: Endometrial Cancer; Hormone Therapy; Breast Cancer; DMBA-induced mammary Cancer; Chemotherapy; Progestogen

An attempt was made to clarify the mechanisms of gestagen therapy on endometrial cancer or breast cancer.Mammary tumor was. induced in female Sprague-Dawley rats by oral administration of 7, 12-dimethybenz[a]anthracene(DMBA). Histologically, the induced mammary tumors were divided into 7 types such as well differentiated adenocarcinoma, moderately differentiated adenocarcinoma and so on. Estrogen receptor(ER)and progesterone receptor(PR)were positively determined in 43(87.8%)and 34(69.4%), respectively, out of 49 samples by the dextran-coated charcoal(DCC)method. A portion of DMBA-induced mammary tumor was transplanted subcutaneously into nude mice in 38 samples. All but one sample which contains neither ER nor PR, could not make tumors. The tumors which were autoimplanted into subcutaneous tissue of the rats did not develop.The primary culture of the tumor have been carried out in 35 times. Though the excellent cell growth of the epithelial cells and fibroblastic cells were seen at the primary stage, no cell lines have been established, because the cell numbers had been tapering at each subculture. We investigated the effects of medroxyprogesterone acetate(MPA)on the tumor developed in nude mice. No apparent antitumor effects were seen. We also investigated antitumor effects of MPA in vitro using the cell strain. There were no difference between the MPA group and the control group.

试图阐明孕激素治疗子宫内膜癌或乳腺癌的机制。乳腺肿瘤。通过口服 7, 12-二甲基苯并[a]蒽 (DMBA) 在雌性 Sprague-Dawley 大鼠中诱导。组织学上将诱发的乳腺肿瘤分为高分化腺癌、中分化腺癌等7种类型。右旋糖酐包被炭(DCC)法检测49份样品中雌激素受体(ER)和孕激素受体(PR)分别呈阳性43份(87.8%)和34份(69.4%)。将DMBA诱导的乳腺肿瘤的一部分皮下移植到38个裸鼠样本中。除一份样品外，所有样品均不含 ER 和 PR，均不能产生肿瘤。自体植入大鼠皮下组织的肿瘤未发育，肿瘤原代培养35次。尽管在初级阶段观察到上皮细胞和成纤维细胞的良好细胞生长，但尚未建立细胞系，因为细胞数量在每次传代培养中逐渐减少。我们研究了醋酸甲羟孕酮（MPA）对裸鼠肿瘤生长的影响。没有看到明显的抗肿瘤作用。我们还使用细胞株研究了 MPA 的体外抗肿瘤作用。 MPA组和对照组之间没有差异。