Chemosensitivity test using immunoprotected culture system in vivo.

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Low molecular weight substances such as glucose can pass through the membrane composed of polylysine and alginate, but the high molecular weight substances such as immunoglobulin can not pass through this membrane. Therefore, microencapsulation of living cells can protect heterotransplanted cells from rejection. This research aims to establish a new chemosensitivity test system using microencapsulated human cancer cells transplanted into mouse in vivo.1.Cell encapsulationThe microcapsules were produced according to the procedure originally reported by Lin, using the Ishikawa cells (an established endometrial adenocarcinoma cell line) for encapsulated cells. Cells were suspended in sodium alginate at a concentration of 1x10^6/ml. This cell suspension formed gelled spherical microdroplets by atomizing into CaCl_2 After coated with polylysine, the gels were washed with sodium alginate and CaCl_2 again. Further washing was employed with sodium citrate to liquify the gel inside the capsules. The adequate size of the capsules with a membrane of polylysine sandwiched between 2 layrs of alginate were 0.5 mm in diameter for manipulation.After several trials and errors, the microcapsules could be produced smoothly.2.Cultivation of the microcapsules in vitroThe capsules were suspended in the culture media and cultured under the closed condition.The phasecontrast microscopic findings revealed that the solitary cancer cells in its early stage grow rapidly foaming aggregates in the microcapsules. The growth curves of the encapsulated cells assessed by SDI analysis showed exponential growth of the cells.