EXPERMENTAL STUDY OF ARTIFICIAL PLACENTA ; DOPPLER ECMO CARDIOGRAPHIC EVALUATION OF GOAT FETAL BLOOD FLOW.

人工胎盘的实验研究；

• 批准号: 05671493
• 负责人: HISANO Katsuya
• 金额: $ 1.15万
• 依托单位: KOBE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671493/
• 关键词: A-V shunt; Artificial placenta; Fetal surgery; Doppler echocardiography; Ductus arteriosus; A-V shunt; fetal surgery; artifical placenta

An expermental study of fetal incubation in artificial amniotic fluid was performed. Four goat fetuses obtained by caesarian incision (average gestational age was 125 days) underwent cannulation through the umbilical arteries and veins. ECMO circuits contained hollowfiber type membrane oxygenators (0.5 m2)and autoregulating sensors. The fetuses were incubated in an artificial amniotic fluid. A-V shunts were performed by a conventional roller pump until cardiac arrest. The duration of ECMO perfusion ranged from 3 to 70 hours with mean of 40 hours. During ECMO perfusion, ductus arteriosus and fetal ductal blood velocity wave forms were evaluated with pulse Dopple echocardiography. Hemodynamics of the fetus undergoing incubation depended on the perfusion rate of ECMO.On A-V ECMO for fetuses, it was advantageous to obtain a flow rate from 80 to 100 ml/kg/min. Ductal occlusion and stenosis changed the blood velocity wave forms, and caused to early death. Doppler echocardiograpic evaluation was the beat way for assessment of fetal circulation in artificial placenta.

进行了人工羊水中胎儿孵化的实验研究。对剖腹产获得的 4 只山羊胎儿（平均胎龄 125 天）进行脐动脉和静脉插管。 ECMO 电路包含中空纤维型膜氧合器 (0.5 m2) 和自动调节传感器。胎儿在​​人造羊水中孵化。通过传统的滚子泵进行 A-V 分流直至心脏骤停。 ECMO灌注时间3～70小时，平均40小时。在ECMO灌注期间，用脉冲多普勒超声心动图评估动脉导管和胎儿导管血速度波形。孵化中胎儿的血流动力学取决于ECMO的灌注速率。对于胎儿的A-V ECMO，获得80至100ml/kg/min的流速是有利的。导管闭塞和狭窄改变了血流速度波形，导致早期死亡。多普勒超声心动图评估是评估人工胎盘中胎儿循环的最佳方式。

项目成果

Hisano K, Okada M, Sakata M, Noma O, Yokoyama N, Uetani Y, Nakamura H: "Experimental study of artificial placenta ; Doppler echocardiographic evaluation of goat fetal bolld flow." Jpn J Artif Organs. 22. 880-884 (1993)

Hisano K、Okada M、Sakata M、Noma O、Yokoyama N、Uetani Y、Nakamura H：“人工胎盘的实验研究；山羊胎儿血流的多普勒超声心动图评估。”

坂田雅宏 他: "Color Doppler法によるECMO A-V Bypass下の山羊胎児血流分布の評価" 人工臓器. 23. 921-924 (1994)

Masahiro Sakata 等人：“使用彩色多普勒方法评估 ECMO A-V 旁路下胎儿山羊的血流分布”人工器官 23. 921-924 (1994)。

Sakata M, Hisano K, Okada M, Yasufuku M, Yokoyama N, Uetani Y, Noma O, Nakamura H: "Experimental study of artificial placenta" Jpn J Artif Organs. 23. 921-924 (1994)

Sakata M、Hisano K、Okada M、Yasufuku M、Yokoyama N、Uetani Y、Noma O、Nakamura H：“人工胎盘的实验研究”Jpn J Artif Organs。

坂田雅宏 他: "Color Duppler法によるECMO A-V Bypass Fの山羊胎児血流分布の評価" 人工臓器. 23 (印刷中). (1994)

Masahiro Sakata 等人：“使用 Color Dupler 方法评估 ECMO A-V Bypass F 中的胎儿山羊血流分布”，Artificial Organs（1994 年出版）。

久野克也 他: "ヤギ胎仔水中保育実験におけるドップラー超音波法を用いた血行動態の研究" 人工臓器. 22. 880-884 (1993)

Katsuya Kuno 等人：“在山羊胎儿水下饲养实验中使用多普勒超声进行血流动力学研究”人工器官。 22. 880-884 (1993)