Possibilities of Organs Growth and Maturation of Fetal Goat Incubated in Artificial Amniotic Fluid (Artificial Placenta)

将8只妊娠约125天（足月，150天）的山羊胎儿置于胎儿ECMO系统中。他们的四个体重相似的双胞胎胎儿被收获并检查作为ECMO前的基线数据（pre-ECMO数据）。A-V ECMO平均持续时间为138.8小时（范围：87 - 237小时）。对所有四个胎儿进行器官生长因子如类固醇激素、甲状腺激素和表皮生长因子的药物治疗。比较4例气管结扎胎儿的肺重量。在ECMO前（双胎）和ECMO期间分析气管液的表面活性剂和电解质。分析ECMO前（双胎）和ECMO后肺组织表面活性物质和肺重量。血浆皮质醇和T3水平也被测定为激素因素，影响肺成熟在pre-ECMO和在ECMO.In的结论，这个胎儿的AV ECMO系统在浴显示出生产的表面活性剂，离子运输的肺上皮细胞的维护，肺重量的增加，并增加成熟的II型细胞，导致肺的生长和成熟。在ECMO后对所有四个胎儿的肺进行称重。ECMO后肺湿重和干重均显著高于ECMO前。ECMO后肺湿重、脑湿重和肝湿重占胎仔体重的比例增加，但与ECMO前相比差异无统计学意义。

Eight goat fetuses at about 125 days' gestation (term, 150 days) were placed in the fetal ECMO system. Their four twin fetuses with similar body weights were harvested and examined as baseline data before ECMO (pre-ECMO data). The mean duration of A-V ECMO was 138.8 hours (range : 87 to 237hours). Medication of organ growth factor such as steroid hormone, thyroid hormon and epitherial growth factor was performed on all four fetuses. The lung weights of the four fetuses with tracheal ligation are compared. The surfactant and electrolytes of the tracheal fluid were analyzed in pre-ECMO (twin fetuses) and during ECMO. The surfactant of lung tissue, and lung weight were analyzed in pre-ECMO (twin fetuses) and after ECMO. Plasma cortisol and T3 levels were also assayed as hormonal factors that effected lung maturation in pre-ECMO and during ECMO.In conclusion, this fetal A-V ECMO systems in the bath showed a production of surfactant, the maintenance of ion transport by the pulmonary epithelium, an increase in lung weight, and an increase in mature type II cells, resulting in lung growth and maturation. he lungs of all four fetuses were weighed after ECMO. Both wet and dry lung weight were significantly higher after ECMO than pre-ECMO. The wet lung, brain and liver weights as proportions of fetal body weights were higher after ECMO, but not significantly differents from pre-ECMO.