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Possibilities of Organs Growth and Maturation of Fetal Goat Incubated in Artificial Amniotic Fluid (Artificial Placenta)

人工羊水（人工胎盘）中孵化的胎山羊器官生长和成熟的可能性

基本信息

批准号：
10671670
负责人：
HISANO Katsuya
金额：
$ 1.54万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671670/
关键词：
artificial placenta membrane oxygenator ECMO organ maturation Artificial placenta fetal development 遠心ポンプ胎児手術 fetal surgery

项目摘要

Eight goat fetuses at about 125 days' gestation (term, 150 days) were placed in the fetal ECMO system. Their four twin fetuses with similar body weights were harvested and examined as baseline data before ECMO (pre-ECMO data). The mean duration of A-V ECMO was 138.8 hours (range : 87 to 237hours). Medication of organ growth factor such as steroid hormone, thyroid hormon and epitherial growth factor was performed on all four fetuses. The lung weights of the four fetuses with tracheal ligation are compared. The surfactant and electrolytes of the tracheal fluid were analyzed in pre-ECMO (twin fetuses) and during ECMO. The surfactant of lung tissue, and lung weight were analyzed in pre-ECMO (twin fetuses) and after ECMO. Plasma cortisol and T3 levels were also assayed as hormonal factors that effected lung maturation in pre-ECMO and during ECMO.In conclusion, this fetal A-V ECMO systems in the bath showed a production of surfactant, the maintenance of ion transport by the pulmonary epithelium, an increase in lung weight, and an increase in mature type II cells, resulting in lung growth and maturation. he lungs of all four fetuses were weighed after ECMO. Both wet and dry lung weight were significantly higher after ECMO than pre-ECMO. The wet lung, brain and liver weights as proportions of fetal body weights were higher after ECMO, but not significantly differents from pre-ECMO.

将8个妊娠约125天（足月150天）的山羊胎儿置于胎儿ECMO系统中。他们收集了四个体重相似的双胞胎胎儿，作为ECMO前的基线数据（ECMO前数据）进行检查。A-V ECMO的平均持续时间为138.8小时（范围：87 ~ 237小时）。4例胎儿均给予类固醇激素、甲状腺激素、上皮生长因子等器官生长因子治疗。比较4例气管结扎胎儿的肺重量。在ECMO前（双胎）和ECMO过程中分析气管液的表面活性剂和电解质。分析ECMO前（双胎）和ECMO后肺组织表面活性物质和肺重量。血浆皮质醇和T3水平也作为影响ECMO前和ECMO期间肺成熟的激素因素进行了检测。综上所述，这种胎儿a - v ECMO系统在泡浴中表现出表面活性剂的产生，肺上皮维持离子运输，肺重量增加，成熟II型细胞增加，导致肺生长和成熟。在体外膜肺氧合后对四个胎儿的肺进行称重。ECMO后干肺和湿肺重量均显著高于ECMO前。ECMO后湿肺、湿脑和湿肝重量占胎儿体重的比例较高，但与ECMO前无显著差异。