LUNG GROWTH AND MATURATION OF FETUS INCUBATED IN ARTIFICIAL PLACENTA

将妊娠约125天的山羊胎儿置于胎儿ECMO系统中。A-V ECMO的平均持续时间为138小时。分析ECMO前和ECMO过程中气管液的表面活性剂和电解质。分析ECMO前后肺组织表面活性物质及肺重量。血浆皮质醇和T3水平作为影响ECMO前和ECMO期间肺成熟的激素因素也被检测。ECMO第5天气管液磷脂、磷脂酰胆碱和卵磷脂鞘磷脂比值均高于ECMO前。ECMO后肺组织磷脂酰胆碱和紊乱磷脂酰胆碱分别为72.3mg/g和19.2mg/g，均显著高于ECMO前。ECMO后肺干、湿重分别为87.8g和6.3g，均显著高于ECMO前。ECMO期间血浆控制水平和T3水平明显高于ECMO前。电镜观察显示，ECMO后肺成熟II型细胞比ECMO前增加。

The goat fetuses at about 125 days gestation were placed in the fetal ECMO system. The mean duration of A-V ECMO was 138 hours. The surfactant and electrolytes of the tracheal fluid were analyzed in pre-ECMO and during ECMO.The surfactant of lung tissue, and lung weight were analized in pre-ECMO and afte ECMO.Plasma cortisol and T3 levels were also assayd as hormonal factors that affected lung maturation in pre-ECMO and during ECMO.The phospholipid, phosphatidylcholine and lecithinsphingomeyelin ratio of the tracheal fluid on day 5 during ECMO were elevated above pre-ECMO.The phosphatidylcholine and disturacted phosphatidylcholine of the lung tissue after ECMO were 72.3mg/g and 19.2mg/g, which were significantly higher than pre-ECMO.The wet and dry lung weights after ECMO was 87.8g and 6.3g, which were significantly higher than pre-ECMO.Plasma control levels and T3 levels during ECMO were significantly higher than pre-ECMO.Electron microscopy demonstrated a higher increase of mature type II cells in the lung after ECMO than pre-ECMO.