LUNG GROWTH AND MATURATION OF FETUS INCUBATED IN ARTIFICIAL PLACENTA

人工胎盘中孵化的胎儿的肺部生长和成熟

• 批准号: 08672057
• 负责人: HISANO Katsuya
• 金额: $ 1.34万
• 依托单位: KOBE UNIVERSITY,SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672057/
• 关键词: FETAL A-V ECMO; ARTIFICIAL PLACENTA; LUNG MATURATION; 肺成熟; artificial placenta; 胎児肺成熟; サーファクタント

The goat fetuses at about 125 days gestation were placed in the fetal ECMO system. The mean duration of A-V ECMO was 138 hours. The surfactant and electrolytes of the tracheal fluid were analyzed in pre-ECMO and during ECMO.The surfactant of lung tissue, and lung weight were analized in pre-ECMO and afte ECMO.Plasma cortisol and T3 levels were also assayd as hormonal factors that affected lung maturation in pre-ECMO and during ECMO.The phospholipid, phosphatidylcholine and lecithinsphingomeyelin ratio of the tracheal fluid on day 5 during ECMO were elevated above pre-ECMO.The phosphatidylcholine and disturacted phosphatidylcholine of the lung tissue after ECMO were 72.3mg/g and 19.2mg/g, which were significantly higher than pre-ECMO.The wet and dry lung weights after ECMO was 87.8g and 6.3g, which were significantly higher than pre-ECMO.Plasma control levels and T3 levels during ECMO were significantly higher than pre-ECMO.Electron microscopy demonstrated a higher increase of mature type II cells in the lung after ECMO than pre-ECMO.

将妊娠约125天的山羊胎儿置于胎儿ECMO系统中。A-V ECMO平均持续时间为138小时。分析ECMO前和ECMO期间气管液的表面活性物质和电解质。分析ECMO前和ECMO后肺组织的表面活性物质和肺重量。血浆皮质醇和T3水平也进行了分析。ECMO前和ECMO期间影响肺成熟的激素因素。磷脂，ECMO期间第5天气管液的磷脂酰胆碱和卵磷脂鞘磷脂比升高，ECMO后肺组织磷脂酰胆碱和干扰磷脂酰胆碱分别为72.3mg/g和19.2mg/g，ECMO后肺湿重和干重分别为87.8g和6.3g，ECMO期间血浆对照水平和T3水平显著高于ECMO前，ECMO。电子显微镜显示ECMO后肺中成熟II型细胞的增加高于ECMO前。

项目成果

Sakata M, Hisano K, et al: "New artificial placeuta with a ceutrifugal punp:long terru total extrauterine support of goat fetus" J of Thoracic Cardiovasular Surgery. (in press). (1997)

Sakata M、Hisano K 等人：“带有 ceutrifugal punp 的新型人工胎盘：山羊胎儿的 long terru 完全宫外支持”《胸心血管外科杂志》。

Sakata M, Hisano K.et al: "New artificial placeuta with a ceutrifugal pump:long term total extrauterine support of goat fetus" J of Thoracic Cardiovasular Surgery. (in press).

Sakata M、Hisano K.等人：“带有离心泵的新型人工胎盘：山羊胎儿的长期完全宫外支持”《胸心血管外科杂志》。

久野克也, 岡田昌義: "新生児のECMO治療" 日本外科学会雑誌. 98(12). 990-995 (1997)

Katsuya Kuno、Masayoshi Okada：“ECMO 治疗新生儿”，日本外科学会杂志 98(12)。

Yasuhuku M, Hisano K et al: "Arterio-venous extracorporeal membraer oxygenation of fetal goat inculated in artificial amniotic fluid" J of Pediatric Surgery. (in press).

Yasuhuku M、Hisano K 等人：“人工羊水中接种的胎山羊的动静脉体外膜氧合”《小儿外科杂志》。

Yasufuku M,Hisano K,Sakata M,Okada M: "Arterio-Venous Extracorporeal Membrane Oxygenation of Fetal Goat Incubated in Artificial Amniotic Fluid (Artificial Placenta) : Influence on Lung Growth and Maturation." Journal of Pediatric Surgery. 33. 442-448 (199

Yasufuku M，Hisano K，Sakata M，Okada M：“人工羊水（人工胎盘）中孵化的胎山羊的动静脉体外膜氧合：对肺生长和成熟的影响。”