ENHANCEMENT OF GROWTH OF PERIODONTOPATHIC BACTERIA BY PRO-INFLAMMATORY CYTOKINES

促炎性细胞因子促进牙周病细菌的生长

• 批准号: 05671520
• 负责人: OCHIAI Tomoko
• 金额: $ 1.34万
• 依托单位: NIHON UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671520/
• 关键词: Growth; Periodontopathic bacteria; IL-1alpha; IL-1beta; IL-6; 歯周病原性 細菌; IL-1beta; IL-1alpha

(1) Effect of cytokines on bacteria growth : we investigated the effect of IL-alpha, beta and IL-6 on bacterial growth. The growth-promoting effect of IL-1alpha, beta over control (no IL-1) was observed during the log phase of Actinobacillus growth (9h : 1.6-1.9 times, 18th : 1.5-1.7 times) . IL-1 alpha at 1 to 100 ng/ml enhanced Actinobacillus growth after 9h incubation compared to control. IL-1beta at 10 and 100 ng/ml promoted Actinobacillus growth. IL-1 alpha, beta also had a smaller effect on Capnocytophaga and Fusobacterium growth.(2) Binding of ^<125>I-IL-1alpha, beta and IL-6 to bacteria : Actinobacillus showed a high level of uptake of the radiolabeled IL-1alpha, beta and IL-6, While Capnocytophaga and Fusobacterium bound a very low amount of ^<125>I-IL-1alpha and IL-6. Other strains including Porphyromonas, Prevotella and Eikenella did not bind to cytokines.(3) Homology search with human cytokine receptor gene : When we examined bacteria for gene expression of cytokine receptor, expression of IL-IR (I) and IL-IR (II) was only detected in Actinobacillus, not other bacteria such as Porphyromonas, Prevotella, Fusobacterium and Capnocytophaga. On the other hand, IL-6R expression was not detected in all the strains used.(4) Autocrine mechanism against bacteria : We examined IL-Ialpha, beta and IL-6 cytokine activity in bacterial culture supernatants. IL-1alpha activity was detected slightly in Fusobacterium and Capnocytophaga, but was not significant. Trace amounts of IL-I beta ware detected in all the strains used, and especially higher level of IL-I beta could be detected in Actinobacillus. IL-6 was not produced in all the strains.

(1)细胞因子对细菌生长的影响：我们研究了IL-α、β和IL-6对细菌生长的影响。在放线杆菌生长的对数期观察到IL-1 α、β相对于对照（无IL-1）的生长促进作用（9 h：1.6-1.9倍，18 th：1.5-1.7倍）。与对照相比，1至100 ng/ml的IL-1 α在孵育9小时后增强放线杆菌的生长。10和100 ng/ml的IL-1 β促进放线杆菌生长。IL-1 α、β对二氧化碳噬纤维菌和梭杆菌生长的影响也较小。(2)β<125>-IL-1 α、β和IL-6与细菌的结合：放线杆菌显示出高水平的放射性标记的IL-1 α、β和IL-6的摄取，而二氧化碳噬细胞菌和梭杆菌结合极少量的<125>β-IL-1 α和IL-6。其他菌株包括卟啉单胞菌、普雷沃氏菌和艾肯氏菌不与细胞因子结合。(3)与人细胞因子受体基因的同源性搜索：当我们检测细菌细胞因子受体的基因表达时，仅在放线杆菌中检测到IL-1 R（I）和IL-1 R（II）的表达，而在其他细菌如卟啉单胞菌属、普雷沃氏菌属、梭杆菌属和二氧化碳噬纤维菌属中未检测到。另一方面，在所有使用的菌株中均未检测到IL-6 R表达。(4)抗细菌的自分泌机制：我们检测了细菌培养上清液中的IL-1 α、β和IL-6细胞因子活性。在梭杆菌属和二氧化碳噬纤维菌属中检测到轻微的IL-1 α活性，但不显著。在所有菌株中均检测到微量IL-1 β，特别是放线杆菌中可检测到较高水平的IL-1 β。所有菌株均不产生IL-6。