ENHANCEMENT OF GROWTH OF PERIODONTOPATHIC BACTERIA BY PRO-INFLAMMATORY CYTOKINES

(1)细胞因子对细菌生长的影响：我们研究了il - α、β和IL-6对细菌生长的影响。放线菌生长对数期（第9h: 1.6 ~ 1.9倍，第18 h: 1.5 ~ 1.7倍）观察到IL-1 α、β较对照（无IL-1）的促生长作用。与对照相比，1 ~ 100 ng/ml的IL-1 α在孵育9h后能促进放线菌的生长。10和100 ng/ml的il -1 β促进放线菌生长。IL-1 α和β对碳吞噬菌和梭杆菌的生长也有较小的影响。(2) <125> i - il -1 α、β和IL-6与细菌的结合：放线菌对放射性标记的il -1 α、β和IL-6的吸收水平很高，而碳吞噬菌和梭杆菌对<125> i - il -1 α和IL-6的吸收水平很低。其他菌株包括卟啉单胞菌、普雷沃氏菌和艾肯菌不与细胞因子结合。(3)与人细胞因子受体基因的同源性研究：检测细胞因子受体基因表达时，IL-IR (I)和IL-IR （II）仅在放线菌（Actinobacillus）中检测到，其他细菌如卟啉单胞菌（Porphyromonas）、普雷沃菌（Prevotella）、梭杆菌（Fusobacterium）和Capnocytophaga等均未检测到。另一方面，所有菌株均未检测到IL-6R的表达。(4)细菌自分泌机制：检测细菌培养上清液中il - α、β和IL-6细胞因子活性。在Fusobacterium和Capnocytophaga中检测到少量il -1 α活性，但不显著。在所有菌株中均检测到微量il - 1 β，其中放线菌中il - 1 β含量较高。所有菌株均未产生IL-6。

(1) Effect of cytokines on bacteria growth : we investigated the effect of IL-alpha, beta and IL-6 on bacterial growth. The growth-promoting effect of IL-1alpha, beta over control (no IL-1) was observed during the log phase of Actinobacillus growth (9h : 1.6-1.9 times, 18th : 1.5-1.7 times) . IL-1 alpha at 1 to 100 ng/ml enhanced Actinobacillus growth after 9h incubation compared to control. IL-1beta at 10 and 100 ng/ml promoted Actinobacillus growth. IL-1 alpha, beta also had a smaller effect on Capnocytophaga and Fusobacterium growth.(2) Binding of ^<125>I-IL-1alpha, beta and IL-6 to bacteria : Actinobacillus showed a high level of uptake of the radiolabeled IL-1alpha, beta and IL-6, While Capnocytophaga and Fusobacterium bound a very low amount of ^<125>I-IL-1alpha and IL-6. Other strains including Porphyromonas, Prevotella and Eikenella did not bind to cytokines.(3) Homology search with human cytokine receptor gene : When we examined bacteria for gene expression of cytokine receptor, expression of IL-IR (I) and IL-IR (II) was only detected in Actinobacillus, not other bacteria such as Porphyromonas, Prevotella, Fusobacterium and Capnocytophaga. On the other hand, IL-6R expression was not detected in all the strains used.(4) Autocrine mechanism against bacteria : We examined IL-Ialpha, beta and IL-6 cytokine activity in bacterial culture supernatants. IL-1alpha activity was detected slightly in Fusobacterium and Capnocytophaga, but was not significant. Trace amounts of IL-I beta ware detected in all the strains used, and especially higher level of IL-I beta could be detected in Actinobacillus. IL-6 was not produced in all the strains.