Chromatological and immunohistochemical study of oral premalignant lesions-Evaluation of mailgnant potentiality-

口腔癌前病变的色谱和免疫组织化学研究-恶性潜能评估-

• 批准号: 05671659
• 负责人: FUJII Eiji
• 金额: $ 1.28万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671659/
• 关键词: premalignant lesion; Oral leukoplakia; malignant potentiality; epithelial dysplasis; the new-type of colorimeter; color difference; degree of cellular atypia; cytokeration (CK) expression pattern

Oral leukoplakia is known as a premalignant lesion. But every leukoplakia doesn't develop malignancy and it may not necessarily be easy to distinguish the leukoplakias with verious levels of malignant potentiality. The aim of this study was to find the effective measures to evaluate the malignant potentiality of leukoplakia objectively and quantitatively.1)As to clinical finding, the color of leukoplakia is closely connected with the malignant potentiality and the leukoplakia with red component has the higher risk. Accordingly the color of the lingual leukoplakia was measured with the new-type of colorimeter without touching the object. The measured color was recorded in figures and the color difference from the normal lingual color was expressed in CIE-LAB system, the three-dimentional standard coordinates (L^*a^*b^*). In general value index (L^*) is high and chromaticness index (a^*, b^*) is low in leukoplakia. But so far as leukoplakia with severe epithelial dyspasia is concerned, the peculiar color (L^* is low and a^*, b^* is high) namely red component made its appearance.2)Concerning histopathological finding, the cytokeratin (CK) expression pattern in oral epithelium is related to the cellular differentiation level. Therefore immunohistochemical analysis of CK expression was thought to be useful aid for the objective evaluation of dysplastic change in leukoplakias. CK10 expression was seen in the epithelium with hyperorthokeratosis and CK13 in the stratum spinosum of the leukoplakia with slight dysplasia. But both CK10 and CK13 were absent in areas with increased degree of cellular atypia. CK high molecular weight, whict includes CK 1,5,10,14, was expressed strongly in the areas where the rete ridge showed irregular elongation. Expression of CK19 was normally seen only in the stratum basale, but also in suprabasal cells in the leukoplakia with severe dysplasia.

口腔粘膜白斑病被称为癌前病变。但并不是所有的白斑都有恶变的可能，不同恶性程度的白斑也不一定容易鉴别。本研究的目的是寻找客观、定量评价白斑恶性潜能的有效方法。1）临床表现方面，白斑颜色与白斑恶性潜能密切相关，红色成分的白斑危险性较高。据此，用新型比色计对舌白斑进行了不接触物体的比色测定。将测得的颜色以数字的形式记录下来，并将与正常舌色的色差以CIE-LAB系统中的三维标准坐标（L^*a^*B^*）表示。一般白斑的明度指数（L^*）较高，色度指数（a^*，B^*）较低。但对于重度上皮发育不良的白斑，出现了特殊的颜色（L^* 低，a^*、B^* 高）即红色成分。2）组织病理学发现，口腔上皮细胞角蛋白（CK）的表达模式与细胞分化程度有关。因此，CK表达的免疫组化分析被认为是有用的援助，以客观评价白斑的不典型性改变。角化过度的上皮细胞中可见CK 10的表达，轻度异型增生的白斑棘层中可见CK 13的表达。而在细胞浸润程度增加的区域，CK 10和CK 13均缺失。CK高分子量蛋白（包括CK 1、5、10、14）在网脊呈不规则伸长的区域表达较强。CK 19的表达仅见于基底层，但在伴有重度异型增生的白斑中，CK 19的表达也见于基底层上细胞。