Purification OF THCA synthase from Cannabis callus
从大麻愈伤组织中纯化 THCA 合酶
基本信息
- 批准号:05671830
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The occurrence of DELTA^1-tetrahydrocannabinolic acid synthase was confirmed in the fresh leaf buds of marihuana (Cannabis sativa L.). The enzyme, assayd with cannabigerolic acid, was purified approximately 300-fold by chromatography on diethylaminoethyl-cellulose, phenyl Sepharose CL-4B and hydroxylapatite. The purified protein migrated during sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 75kD.The native molecular mass determined by gel-filtration chromatography was 74 kDa, indicating that DELTA^1-tetrahydrocannabinolic acid synthase is a monomeric enzyme. Using isoelectric focusing, we determined a pI of 6.4 for the enzyme. The enzyme activity was greatest between pH5.5 and 6.0. The enzyme catalyzed the conversion of cannabigerolic acid or cannabinerolic acid to DELTA^1-tetrahydrocannabinolic acid. The V_<max> values were 2.68 and 0.37 nkat/mg for cannabigerolic acid and cannabinerolic acid, respectively. These results indicated that DELTA^1-tetrahydrocannabinolic acid is predominantly formed from cannabigerolic acid.
在大麻(Cannabis sativa L.)的新鲜叶芽中证实了Δ ^1-四氢大麻酚酸合成酶的存在。用大麻萜酚酸测定的酶,经二乙氨基乙基纤维素、苯基琼脂糖CL-4 B和羟基磷灰石层析纯化约300倍。SDS-PAGE电泳结果表明,该酶为单一条带,分子量约为75 kD,凝胶过滤层析结果表明,该酶的天然分子量为74 kD,表明该酶为单体酶。使用等电聚焦,我们测定了该酶的pI为6.4。在pH5.5 ~ 6.0范围内酶活性最高。该酶催化大麻萜酚酸或大麻酚酸转化为Δ ^1-四氢大麻酚酸。大麻<max>萜酚酸和大麻酚酸的V_值分别为2.68和0.37 nkat/mg。这些结果表明,Δ ^1-四氢大麻酚酸主要由大麻萜酚酸形成。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Futoshi Taura et al.: "New cannabinoid, cannabinerolic acid" from Cannabis sativa Phytochemistry. (in press). (1995)
Futoshi Taura 等人:“新大麻素,大麻酚酸”来自大麻植物化学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
FutoSHI TAURA: "A New Cannabinoids,Cannabinerlic Acid,from Cannabis Sativa" Phytochemistry. (1995)
FutoSHI TAURA:“一种新的大麻素,大麻二酸,来自大麻”植物化学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
FutoSHI TAURA: "A New Cannabinoid,Cannabinerolic acid,from Cannabis Sativa" Phytochemistry. (1995)
FutoSHI TAURA:“一种新的大麻素,来自大麻的大麻酚酸”植物化学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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MORIMOTO Satoshi其他文献
MORIMOTO Satoshi的其他文献
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18K18139 - 财政年份:2018
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Molecular- and structural-biological studies on plant necrosis
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14572110 - 财政年份:2002
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10672108 - 财政年份:1998
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