Molecular- and structural-biological studies on plant necrosis

植物坏死的分子和结构生物学研究

• 批准号: 20590123
• 负责人: MORIMOTO Satoshi
• 金额: $ 3万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590123/
• 关键词: 薬用資源学; ネクローシス; カンナビノイド; ミトコンドリア; モノクローナル抗体; Cyclophilin D; VDAC; THCA

Two cannabinoids were identified as plant necrosis-inducing factors from Cannabis sativa. In addition, genes encoding cyclophilin D (CYD) and voltage-dependent anion channel (VDAC), which are assumed to be involved in necrosis, were cloned and expressed in E. coli. Anti-VDAC antibodies were prepared using recombinant VDAC. Animal VDAC antibodies are known to inhibit necrosis in animal cells, whereas the above VDAC antibodies obtained in my study had such inhibitory effect for plant cells.

从大麻中鉴定出两种大麻素类物质为植物坏死诱导因子。此外，还克隆了编码亲环素D（CYD）和电压依赖性阴离子通道（VDAC）的基因，并在大肠杆菌中表达。杆菌使用重组VDAC制备抗VDAC抗体。已知动物VDAC抗体抑制动物细胞中的坏死，而在我的研究中获得的上述VDAC抗体对植物细胞具有这种抑制作用。

项目成果

カンナビノイドの生合成に関する化学的・生物学的研究

大麻素生物合成的化学和生物学研究

• 发表时间: 2009
• 作者: Y. Shoyama;et al.;森元聡
• 通讯作者: 森元聡

Cannabinoids act as necrosis-inducing factors in Cannabis sativa

大麻素是大麻中的坏死诱导因子

• 发表时间: 2008
• 期刊: Plant Signaling & Behavior 3
• 作者: Y. Shoyama;et al.
• 通讯作者: et al.