Structural and biological studies on metabolism of active oxygen species in medicinal plants

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Hydrogen peroxide, which occurs in plant cells, is known to give serious stress to plant bodies. Many plants construct the systems to detoxify hydrogen peroxide in their bodies, and peroxidases catalyze this detoxification. To precisely understand the mechanism of these detoxifying reactions, I attempted to demonstrate the structures of morphine peroxidase and baicalein peroxidase by X-ray crystallography. As results, I expressed large amounts of recombinant protein in E. coli, whereas they were insoluble and lost the peroxidase activity. Therefore, when I refolded the insoluble and inactive protein, and obtained both peroxidases in the active forms. In addition, we crystallized morphine peroxidase.