Development of an oligonucleotide dosimeter for absolute measurements of harmful solar UV radiation

开发用于绝对测量有害太阳紫外线辐射的寡核苷酸剂量计

• 批准号: 05680468
• 负责人: HIEDA Kotaro
• 金额: $ 1.22万
• 依托单位: Rikkyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680468/
• 关键词: Solar UV; DNA; photoproduct; dinucleotide

Since the most important molecular damage leading to biological effects induced by solar UV radiation is DNA photoproducts, the amount of DNA photoproducts must be a direct measure of the biologically effective dose of solar UV radiation. An oligonucleotide, 2'-deoxythymidylyl-(3'-5')-2'-deoxythymidine(dTpdT), has been chosen as the simplest model molecule suitable for this purpose, since its photoproducts can be easily quantitated by HPLC.The results are summarized as follows. (1) The actinometer measuring solar UV radiatio by cis-syn dimers in dTpdT solution has been developed. (2) Pilot field measurements for harmful solar UV radiation at Rikkyo University showed that the dTpdT dosimeter was an easily and accurate dosimeter. (3) Physically measured UVB amounts were different from those using the dTpdT dose by several times, indication that the direct measurement of DNA photoproducts is important for estimating biologically effective dose of solar UV radiation.

由于导致太阳紫外线辐射生物效应的最重要的分子损伤是DNA光产物，因此DNA光产物的量必须直接衡量太阳紫外线辐射的生物有效剂量。作为一种寡核苷酸，2‘-deoxythymidylyl-(3’-5‘)-2’-deoxythymidine(dTpdT)，被选为最简单的适合于这一目的的模型分子，因为它的光产物可以很容易地用HPLC定量。(1)研制了测量dTpdT溶液中顺式二聚体太阳紫外辐射的辐射计。(2)在理光大学进行的有害太阳紫外线辐射的中试现场测量表明，dTpdT剂量计是一种简单、准确的剂量计。(3)物理测量的UVB量与dTpdT剂量相差数倍，表明直接测量DNA光产物对估算太阳紫外线辐射的生物有效剂量具有重要意义。

项目成果

M. Satitou and K. Hieda: "Dithymine photodimers and photodestruction products of thymidylyl-thymidine induced by ultraviolet radiation from 150 to 300nm." Radiat. Res.,. 140. 215-220 (1994)

M. Satitou 和 K. Hieda：“150 至 300 nm 紫外线辐射诱导的二胸腺嘧啶光二聚体和胸苷酰胸苷的光破坏产物。”

K.Hieda and Y.Yamaguchi: "Development of a dosimeter for absolute measurements of harmful solar UV radiation using deoxythymidylyl-5'-3'-thymidine (Japanese)." Proceedings of the Japanese Committee for Sunlight Protection. Vol.5, No.2. 19-27 (1995)

K.Hieda 和 Y.Yamaguchi：“开发剂量计，使用脱氧胸苷基-5-3-胸苷（日语）绝对测量有害太阳紫外线辐射。”

T.Hara, M.Saitou, and K.Hieda18GB04 : Boundary wavelength between far-UV and X-ray types of DNA damage.: Proceedings of 10th International Congress of Radiation Research. (in press).(1996)

T.Hara、M.Saitou 和 K.Hieda18GB04：远紫外线和 X 射线类型 DNA 损伤之间的边界波长。：第 10 届国际辐射研究大会论文集。

檜枝光太郎、山口由起子: "Deoxythymidylyl-5′-3′-thymidine(TpT)を用いた有害太陽紫外線の絶対線量計" 太陽紫外線防御研究委員会学術報告書. 5巻2号. 19-27 (1995)

Kotaro Hidea、Yukiko Yamaguchi：“使用脱氧胸苷基-5-3-胸苷 (TpT) 的有害太阳紫外线绝对剂量计”，太阳紫外线防护研究委员会的科学报告，第 5 卷，第 2. 19-27 期。 1995）

N.Munakata, F.Moroboshi, K.Hieda, K.Suzuki, Y.Furusawa, H.Shimura, and T.Ito: "Experimental correspodence between spore dosimetry and spectral phtometry of solar ultraviolet radiation." Photochem. Photobiol.63. 74-78 (1996)

N.Munakata、F.Moroboshi、K.Hieda、K.Suzuki、Y.Furusawa、H.Shimura 和 T.Ito：“孢子剂量测定与太阳紫外线辐射光谱光度测定之间的实验对应关系。”