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Studies of biological effects of vacuum ultraviolet radiation above 50 nm.

50 nm以上真空紫外辐射的生物效应研究。

基本信息

批准号：
61304066
负责人：
HIEDA Kotaro
金额：
$ 4.22万
依托单位：
Rikkyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The monochromatic vacuum-ultraviolet (VUV) irradiation system for radiobiological studies installed at BL-5 of the SOR-RING, the Institute for Solid State Physics, the University of Tokyo, was improved as follows. Firstly a 50 1/s ion pump was added to the grating chamber of the monochromator to obtain better vacuum. As a result, decrease of light intensity due to deteriotation of the grating became negligible. Secondly a shutter was replaced by a new high-performance type, which can control irradiation time down to 0.25 sec. Thirdly computor program was improved. The following experiments were performed during 1986 and 1987 fiscal years.Dry samples: Oligonucleotide, dephospho (dA)_2, was irradiated over 55 to 190 nm range and analyzed thin-layer chromatography (T. Ito et al.). Following DNA damages were measured: single-strand breaks detected by agarose gel electrophoresis by K. Hieda (80-255 nm), base release detected by HPLC by K. Hieda (150-190 nm) and thymine-dimer detected by monoclonal antibody by O. Nikaido (150-260 nm). Inactivation of lysozyme by K. Kobayashi for 60-190 nm, inactivation of T_1 phage by H. Maezawa for 50-260 nm and inactivation of Batillus subtilis spore by N. Munakata for 50-300 nm were measured. Conventional absorption measurement on lipid bilayer by T. Megumi and photoacoustic spectrometry of organic film by T. Inagaki were also performed.Solution: OH radical produced by VUV was measured by the spin-trapping method by A. Minegishi. Photolysis of dinucleotide, CpC, by K. Takakura for 150-254 nm.

安装在东京大学固态物理研究所SOR-RING BL-5的用于放射生物学研究的单色真空紫外线辐照系统作了如下改进。首先在单色器的光栅室中增加了一个50 l/s的离子泵，以获得更好的真空度。结果，由于光栅的旋转而引起的光强度的降低变得可以忽略不计。其次，用一种新的高性能类型取代了快门，它可以将照射时间控制在0.25秒。第三，改进了计算机程序。在1986和1987财政年度期间进行了以下实验：干样品：寡核苷酸，脱磷酸（dA）_2，在55 - 190 nm范围内照射，并进行薄层色谱分析（T。Ito等人）。DNA损伤检测：琼脂糖凝胶电泳检测单链断裂; Hieda（80-255 nm），通过HPLC由K. Hieda（150-190 nm）和O. Nikaido（150-260 nm）。K.小林在60-190 nm范围内对T_1噬菌体的灭活作用。Maezawa的50-260 nm的紫外线照射和枯草芽孢杆菌（Bacillussubtilis）孢子的灭活。测量50-300 nm的Munakata。用T. Megumi和T.溶液：采用自旋捕获法测量真空紫外产生的OH自由基。峰岸二核苷酸CpC被K. Takakura为150-254 nm。