STRUCTURAL IDENTIFICATION AND CHARACTERIZATION OF A NEW DNA PHOTOPRODUCT

新型 DNA 照片产品的结构鉴定和表征

• 批准号: 8361374
• 负责人: JOHN-STEPHEN Adolfino TAYLOR
• 金额: $ 2.68万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361374
• 关键词: Attention; Cytosine; DNA biosynthesis; DNA photoproducts; Deamination; Funding; Goals; Grant; High Pressure Liquid Chromatography; Kinetics; Link; Mass Spectrum Analysis; Measures; National Center for Research Resources; Preparation; Principal Investigator; Pyrimidine Dimers; Research; Research Infrastructure; Resources; Site; Skin Cancer; Source; Structure; Structure-Activity Relationship; Techniques; Time; Ultraviolet B Radiation; United States National Institutes of Health; X-Ray Crystallography; biomedical resource; cost; design; dimer; irradiation; sunlight-induced; transition mutation; ultraviolet irradiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA synthesis past deamination products of cytosine and 5-methylcytosine-containing cis-syn pyrimidine dimer photoproducts may be one of the principal mechanisms by which sunlight induces C-to-T transition mutations linked to skin cancer. To understand better the structure-activity relationships governing photoproduct deamination, we designed the ODN sequence d(GTATCATGAGGTGC) containing a unique TC site to enable preparation of a dT[c,s]C dimer by UV irradiation to measure the kinetics of deamination of cytosine photoproducts. Instead of the expected T[c,s]C photoproduct, an abundant unknown with an unusually short HPLC retention time attracted our attention during the course of UVB irradiation (312 nm) of d(GTATCATGAGGTGC). This product only appeared at low pH (below 6). Our goal is to elucidate the structure of this unknown major photoproduct by combining various techniques such as HPLC, mass spectrometry, NMR, and X-Ray crystallography.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 DNA合成超过胞嘧啶和含5-甲基胞嘧啶的顺式嘧啶二聚体的脱氨基产物可能是阳光诱导与皮肤癌相关的C-T转换突变的主要机制之一。为了更好地了解控制光产物脱氨反应的构效关系，我们设计了含有唯一TC位点的ODN序列d(GTATCATGAGGTGC)，使其能够在紫外光照射下制备DT[c，S]C二聚体，以测量胞嘧啶光产物的脱氨动力学。在d(GTATCATGAGGTGC)的UVB(312 Nm)照射过程中，除了预期的T[c，S]C光产物外，一个具有异常短的保留时间的丰富的未知物质引起了我们的注意。该产品仅在低pH值(6以下)下出现。我们的目标是通过结合各种技术，如高效液相色谱、质谱学、核磁共振和X射线结晶学来阐明这一未知的主要光产物的结构。