Microbial Degradation of Dioxin and Its Related Compounds

二恶英及其相关化合物的微生物降解

• 批准号: 05680482
• 负责人: OMORI Toshio
• 金额: $ 1.28万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680482/
• 关键词: dibenzofuran; dioxin; meta ring-fission enzyme; fluorene; microbial degradation; シベンゾフラン; フレオレン; ジベンゾパラダイオキシン; ジベンゾチオフェレ

To elucidate the genes of the enzymes including initial dioxygenases involved in the degradation pathways of dioxin and dibenzofuran by strain DBF63, the cloning of the genes and sequencing of the isolated genes were carried out.One clone (pLM1) specifing meta cleavage enzyme has been isolated. Another gene for meta cleavage enzyme was also found out. The isolated gene for meta cleavage enzyme has Sph1 site. Restriction enzyme Sph1 was used to obtain another gene by shot gun cloning with use of host vector system of E.coli MV1184 and pUC119. An active tageted clone was detected by spraying the solution mixture of catechol, 3-methylcatechol, 4-methylcatechol and 3-phenylcatechol which are analogus compouds of substrate 2,2', 3-trihydroxybiphenyl to the colonies of transformants.The newly obtained plasmid pKN1 has completely different restriction enzyme site with the other clone which has activity of meta ring-fission enzyme.

为阐明DBF 63降解二恶英和二苯并呋喃的起始双加氧酶基因，对DBF 63降解二恶英和二苯并呋喃的起始双加氧酶基因进行了克隆和序列测定，获得了一个特异性Meta裂解酶的克隆（pLM 1）。还发现了另一个Meta裂解酶基因。分离的Meta切割酶基因具有Sph 1位点。用限制性内切酶Sph 1，利用大肠杆菌MV 1184和pUC 119宿主载体系统，通过鸟枪克隆获得另一个基因。将底物2，2 '，3-三羟基联苯的类似物邻苯二酚、3-甲基邻苯二酚、4-甲基邻苯二酚和3-苯基邻苯二酚的混合溶液喷向转化子菌落，检测到一个活性标签克隆，新获得的质粒pKN 1与另一个具有Meta环裂合酶活性的克隆具有完全不同的限制性酶切位点。

项目成果

門奈 他: "Microbial Degradation of Dibenzo-p-dioxin,Dibenzo furan and Fluorene by staphy lococcus a uriculans Strain DBF63" Appl.Environ.Microbiol.58. 911-915 (1992)

Monna 等人：“尿葡萄球菌菌株 DBF63 对二苯并-对-二恶英、二苯并呋喃和芴的微生物降解”Appl.Environ.Microbiol.58 (1992)。

Monna, L., et al.: "Microbial Degradation of Dibenzo-p-dioxin, Dibenzofuran and Fluorene by Staphylococcus auricularis Strain DBF63" Appl.Environ.Microbiol.59. 285-289 (1993)

Monna, L. 等人：“耳廓葡萄球菌菌株 DBF63 对二苯并-对-二恶英、二苯并呋喃和芴的微生物降解”Appl.Environ.Microbiol.59。

Monna, L. et al.: "Microbial Degradation of Dibenzu-p-dioxin Dibenzufuran and Fluorene by Staphlococcos auriculr's Strain DBF63" Appl. Ennron, Microbiol. 59. 285-289 (1993)

Monna, L. 等人：“Staphlococcos auriculr 菌株 DBF63 对 Dibenzu-p-dioxin Dibenzufuran 和芴的微生物降解”Appl。

Lisa Monna: "Microbial Degradation of Dibenzofuran,Fluore,and Dibenzo-p-Dioxin" Appl.Environ.Microbiol.59. 285-289 (1993)

Lisa Monna：“二苯并呋喃、氟和二苯并-对二恶英的微生物降解”Appl.Environ.Microbiol.59。