DYNAMIC ASPECTS OF SPECIFIC RECOGNITION PROCESSES AT LIPID MEMBRANE INTERFACES

脂质膜界面特异性识别过程的动态方面

• 批准号: 06453153
• 负责人: KITANO Hiromi
• 金额: $ 0.77万
• 依托单位: TOYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453153/
• 关键词: SURFACE-ENHANCED RAMAN SCATTERING (SERS); MEMBRANE PROTEINS; GLYCOLIPID; MULTIPLE INTERNAL REFLECTION FLUORESCENCE (MIRF); MOLECULAR RECOGNITION; LECTIN; HAPTEN; レクチン; スピン・格子緩和時間; 脂質二分子膜小胞; 細胞膜; 表面増強共鳴ラマン法; シクロデモストリン; 視覚化手法

DYNAMIC ASPECTS CONCERNING SPECIFIC MOLECULAR RECOGNITION PROCESSES AT LIPID MEMBRANE INTERFACES WERE INVESTIGATED BY MAKING USE OF VIBRATION SPECTROSCOPY (SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) AND FOURIER-TRANSFORM INFRA RED (FTIR) SPECTROSCOPY,FOR EXAMPLE), MULTIPLE INTERNAL REFLECTION FLUORESCENCE METHOD (MIRF), AND MICROSCOPIC METHOD.(1) CYTOCHROME C BOUND TO SELF-ASSEMBLED MONOLAYERS (SAM) ON SILVER WAS STUDIED BY SERS,AND IT WAS CONCLUDED THAT SILVER MODIFIED WITH A SAM IS A SUITABLE SERS-ACTIVE SUBSTRATE TO ANALYZE THE STRUCTURE OF PROTEINS ON BIOMIMETIC MEMBRANE SURFACES WITHOUT DENATURATION.(2) NOVEL AMPHIPHILES WHICH CONTAIN GALACTOSE RESIDUES WERE PREPARED BY TELOMERIZATION OF GALACTOSE-CARRYING VINYL MONOMER USING A LIPOPHILIC RADICAL INITIATOR.THE GALACTOSE-CARRYING AMPHIPHILES INCORPORATED IN A LIPOSOME WAS EASILY RECOGNIZED BY A LECTIN FROM Ricinus communis AND GALACTOSE OXIDASE.THE RECOGNITION WAS LARGELY AFFECTED BY THE DEGREE OF POLYMERIZATION AND THE SURFACE DENSITY OF THE AMPHIPHILE.(3) BINDING PROCESSES OF ANTIBODY TO A HAPTEN-DOPED LIPID MONOLAYERS WERE FOLLOWED BY THE MULTIPLE INTERNAL REFLECTION FLUORESCENCE METHOD.THE INITIAL RATE OF BINDING OF THE ANTIBODY INCREASED AT A LOW SURFACE DENSITY OF HAPTENS IN THE LIPID MONOLAYER,AND LEVELED OFF AT A MODERATE-TO-HIGH HAPTEN DENSITY.THE LONG SPACER BETWEEN THE HAPTEN GROUP AND THE LIPID LAYER PROMOTED THE ACCESS OF ANTIBODIES TO THE HAPTENS.

用振动光谱(如表面增强拉曼光谱(SERS)和傅立叶变换红外光谱(FTIR))、多重内反射荧光法(MIRF)和显微方法研究了脂膜界面特定分子识别过程的动态变化：(1)用SERS研究了细胞色素C与银表面自组装单分子膜(SAM)的结合；(2)通过亲脂性自由基引发半乳糖乙烯基单体的TELOMERIATE反应，制备了新型的含有半乳糖残基的两亲分子。半乳糖修饰的两亲分子很容易被蓖麻凝集素和半乳糖氧合酶识别。识别能力受聚合度和两亲分子表面密度的影响较大。(3)抗体与半乳糖掺杂的脂单分子膜的结合过程遵循多重内反射荧光法。抗体的初始结合率在脂单分子层中半抗原的表面密度较低时增加，半抗原组与脂层之间的长间隔区促进抗体对半抗原的访问。

项目成果

野崎知行.前田寧.伊藤研策.北野博巳: "Cydosextrins Modified with Polguen chains" Mauoms lecules. 28. 522-524 (1995)

Tomoyuki Nozaki。Yasushi Kensaku Ito。“用 Polguen 链修饰的环糊精”Mauoms lecules。

北野博巳 大野工司: "Sugar-Cantaining Lipids Prepand by Using a Lipophinki Initiator" Langminr. 10. 4131-4135 (1994)

Hiromi Kitano 和 Koji Ohno：“使用 Lipophinki 引发剂制备含糖脂质”Langminr。10. 4131-4135 (1994)

大野工司.北野博巳ら: "Galactose-Containing Amphiphiles Prepared with a Lipophilic Radical Initiator : Association Processes between Liposomes Triggered by Enzymatic Reaction" Bioconjugate Chemistry. 6. 361-366 (1995)

K. Ohno、Hiromi Kitano 等人：“用亲脂性自由基引发剂制备的含半乳糖两亲物：酶促反应引发的脂质体之间的缔合过程”《生物共轭化学》6. 361-366 (1995)。

谷本智史.北野博巳: "Binding Kinetics of Antibody to Hapten-Doped Lipid Monolayers As Studied by Multiple Internal Reflection Fluorescence Method" Colloids and Surfaces. 4. 259-265 (1995)

Satoshi Tanimoto. Hiromi Kitano：“通过多重内反射荧光方法研究抗体与半抗原掺杂脂质单层的结合动力学”胶体和表面。 4. 259-265 (1995)

永山一美: "Catalytic Propertres of Enzymes Modified with Polymers" Macrmol.Chem.Phys.196. 611-620 (1995)

Kazumi Nagayama：“聚合物修饰酶的催化特性”Macrmol.Chem.Phys.196（1995）。