Regulation mechanism by blue light of photomorophogenesis and brassinosteroid activity

蓝光对光形态发生和油菜素类固醇活性的调节机制

• 批准号: 06453160
• 负责人: ABE Hiroshi
• 金额: $ 4.29万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453160/
• 关键词: Blue Light; Myxobacteria; Brassinosteroid activity; Photomorphogenesis; Carotenoid biosynthesis; Photosignal transduction; シグナルトランスダクション; ブラシノステロイド; カロチノイド

This is study on photoregulation mechanism by blue light of lamina inclination of etiolated rice seedlings and formation of fruiting body of Myxobacteria. Lamina inclination of etiolated rice seedlings was remarkably promoted by treatment with brassinosteroid (BS), the six-type of phytohormone, at a extremely low concentration and by irradiation with blue light. A lamina inclination activity in the seedlings grown under blue light irradiation was enhanced about 30 times higher than that grown under the darkness. The blue light effect was observed within 3 hr of irradiation and the same effect was maintained after 48 hr irradiation. However, the endogenous levels of BSs which were identified to be teasterone, typhasterol, castasterone and brassinolide. Enhancement of the inclination activity was caused by presence of a BS synergist. The inclination activity of seedlings grown under the darkness was recovered by means of HPLC purification, resulting presence of a certain suppressor that … More inhibits the function of BS synergist in the dark-grown seedlings. From the facts it was shown that the blue light effect is to play a role of decomposing or removing the suppressor of the BS synergist. Both of the BS synergist and its suppressor failed to identify because of a quite small amounts and unstable compound. But we found a BS synergist during screening some synthetic compounds. Probably it will offer any chemical informations for identification of the natural compound.On the other hand, blue light is essential for formation of fruiting body in a life cycle of Myxobacteria which is most evolutionary in gram negative bacteria. An inhibitor of carotenoid biosynthesis inhibited forming the fruiting body under blue light irradiation. Effect of blue light was to changing qualitatively in carotenoid accumulation at the beginning of the fruiting formation. Carotenoid commonly distributed in plant tissues has been counted to be one of a possible receptor of blue light. Assignment of a factor inducing the fruiting body formation of Myxococcus will offer very useful information as to photomorphogenesis of plants. Less

本研究是蓝光对黄化稻苗叶片倾斜度和粘细菌子实体形成的光调节机制的研究。通过极低浓度的六种植物激素油菜素类固醇（BS）处理和蓝光照射，黄化水稻幼苗的叶片倾斜度显着提高。在蓝光照射下生长的幼苗的叶片倾斜活性比在黑暗下生长的幼苗增强约30倍。在照射后3小时内观察到蓝光效应，并且在照射48小时后仍保持相同的效应。然而，内源水平的 BS 被鉴定为茶甾酮、伤寒甾醇、蓖麻甾酮和油菜素内酯。倾向活性的增强是由BS增效剂的存在引起的。通过HPLC纯化，恢复了黑暗下生长的幼苗的倾向活性，导致存在某种抑制物，该抑制剂抑制了黑暗生长的幼苗中BS增效剂的功能。事实证明，蓝光效应是起到分解或去除BS增效剂抑制剂的作用。 BS增效剂及其抑制剂均因含量极小且不稳定而未能鉴定。但我们在筛选一些合成化合物时发现了BS增效剂。它可能会提供用于鉴定天然化合物的任何化学信息。另一方面，蓝光对于粘细菌（革兰氏阴性细菌中进化程度最高）的生命周期中子实体的形成至关重要。类胡萝卜素生物合成抑制剂在蓝光照射下抑制子实体的形成。蓝光的作用是在子实体形成初期改变类胡萝卜素积累的质量。通常分布在植物组织中的类胡萝卜素被认为是蓝光的可能受体之一。诱导粘球菌子实体形成的因子的分配将为植物的光形态发生提供非常有用的信息。较少的

项目成果

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SEIICHI ASAKAWA：“百合花药和 Distylium racemosum 叶的纯化和鉴定及其生物转化为 Typhasterol。”

YOSHIMASA KYOKAWA: "Synergistic Effect of Sterol Glucoside on Brassinolide Activity in Lamina Inclination and Epicotyl Elongation." J.Pesticide Science. 21 (in press). (1996)

YOSHIMASA KYOKAWA：“甾醇葡萄糖苷对叶片倾斜和上胚轴伸长中油菜素内酯活性的协同作用。”

S.Kyokawa,et al: "Synergistic Effect of Sterol Glucoside on Brassinolide Activity in Lamina Inclination and Epicotyl Elongation" J.Pesticide Science.21(2)(in press). (1996)

S.Kyokawa 等人：“甾醇葡萄糖苷对叶片倾斜和上胚轴伸长中油菜素内酯活性的协同作用”J.Pesticide Science.21(2)（出版中）。

S.Asakawa et al: "Purification and Indentification in Lily Anther and Distyliumracemosum Leaves and its Biotransformation into Typhasterol" Bioso.Biotech Biochem.in press. (1996)

S.Asakawa 等人：“百合花药和 Distyliumracemosum 叶的纯化和鉴定及其生物转化成香蒲甾醇”Bioso.Biotech Biochem.in press。

安倍浩: "ブラシノステロイド研究のフロンティアー生理・生合成・ストレス耐性への関与など新展開" 化学と生物. 34. 356-358 (1994)

Hiroshi Abe：“油菜素类固醇前沿研究的新进展，包括其在生理学、生物合成和抗应激方面的作用。” 34. 356-358 (1994)。