Two-oscillator coupling in circadian clock

生物钟中的两个振荡器耦合

• 批准号: 16590173
• 负责人: ABE Hiroshi
• 金额: $ 2.3万
• 依托单位: University of Fukui (2005)Hokkaido University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590173/
• 关键词: Circadian rhythm; Biological clock; Suprachiasmatic nucleus; Behavior; Clock gene; CS mouse; Rhythm splitting; Methamphetamine; リズムスプリッティング; 制限給餌サイクル

Two-oscillator model of circadian clock has been suggested to be 1) Evening (E) and Morning (M) oscillators, 2) master pacemaker in the suprachiasmatic nucleus (SCN) and food-entrainable oscillator (FEO) under restricted feeding schedule (RF), and 3) the SCN pacemaker and methamphetamine (MAP)-induced oscillator. Present study examined the functional relationship between E/M oscillators, FEO, and MAP oscillator by using CS mouse which shows 'splitting' in the circadian rhythm of behavior and obtained following results.1. To establish the stable condition which induces 'splitting' in behavioral rhythm of CS mouse, three modified light-dark conditions (LD6:6:6:6 h, LD9:6:3:6 h and LD9:15 h) were tested for the mouse housed in a running-wheel cage. However, CS mice did not show splitting in the running-wheel activity rhythm under these conditions. This indicates that constant darkness (DD) is the best condition to induce 'splitting' in the circadian rhythm of CS mouse.2. Under RF (3 h feeding) in DD, behavioral rhythm of CS mouse was entrained by RF. In addition, clock gene (mPer1, mPer2 and mBMAL1) expression rhythms in the SCN were entrained by RF. These results indicate that the SCN pacemaker of CS mouse is entrained by RF unlike that of rats and other strain of mouse (C57BL/6J). During RF and the ad lib feeding after RF, behavioral rhythm did not show 'splitting', suggesting that the E/M oscillator might be associated with FEO in the CS mouse.3. Administration of 0.01% MAP in drinking water under DD induced the activity component which freeran with lengthened period in addition to the freerunning component of split rhythm. After MAP administration, M component of split rhythm disappeared. These results suggest that there is no relationship between E/M oscillators and MAP oscillator, and that the M oscillator might be influenced by MAP administration.

昼夜节律钟的双振子模型被认为是：1）夜间（E）和早晨（M）振子; 2）限制进食时视交叉上核（SCN）的主起搏点和食物敏感振子（FEO）;本研究利用行为昼夜节律分裂的CS小鼠，探讨了E/M振荡器、FEO和MAP振荡器之间的功能关系，得到以下结果.为了建立诱导CS小鼠行为节律“分裂”的稳定条件，对饲养在转轮笼中的小鼠进行了三种改良的光暗条件（LD 6：6：6：6 h，LD 9：6：3：6 h和LD 9：15 h）的测试。然而，CS小鼠在这些条件下没有表现出分裂的转轮活动节奏。这表明恒定黑暗是诱导CS小鼠昼夜节律“分裂”的最佳条件.在DD条件下，RF（3 h喂养）诱导CS小鼠的行为节律。此外，时钟基因（mPer 1，mPer 2和mBMAL 1）在SCN中的表达节律被RF所诱导。这些结果表明，CS小鼠SCN起搏点与大鼠和其他品系小鼠（C57 BL/6 J）不同，是由RF携带的。在RF期间和RF后的自由进食期间，行为节律没有出现“分裂”，提示CS小鼠的E/M振荡器可能与FEO有关. 0.01%MAP饮水给药在DD条件下，除诱发分裂节律的自由运行成分外，还诱发了周期延长的自由运行成分。MAP给药后，分裂节律的M成分消失。这些结果表明，E/M振荡器和MAP振荡器之间没有关系，M振荡器可能受到MAP的影响。

项目成果

Circadian rhythms in behavior and clock gene expressions in the brain of mice lacking histidine decarboxylase

• DOI: 10.1016/j.molbrainres.2004.02.015
• 发表时间: 2004-05-19
• 期刊: MOLECULAR BRAIN RESEARCH
• 作者: Abe, H;Honma, S;Honma, K
• 通讯作者: Honma, K

時間遺伝子によるリズム発振:主時計と末梢時計の脱同調

时间基因引起的节律振荡：主时钟与外设时钟不同步

• 发表时间: 2004
• 期刊: 臨床脳波 46卷4号
• 作者: Sakai;T.;安倍 博
• 通讯作者: 安倍 博