Structure and function of a novel fungal enzyme, lactonohydrolase, and its application to optical resolution of pantolactone

新型真菌酶内酯水解酶的结构和功能及其在泛内酯光学拆分中的应用

• 批准号: 06453167
• 负责人: SHIMIZU Sakayu
• 金额: $ 5.06万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453167/
• 关键词: Pantolactone; Optical resolution; Lactonase; Fusarium oxysporum; High-mannose sugarchain

A fungal lactonohydrolase catalyzes the stereospecific hydrolysis of the intramolecular ester bond of D-pantoyl lactone and is useful for optical resolution of racemic pantoyl lactone. High activity of this stereospecific hydrolysis reaction was found in several filamentous fungi belonging to the genera Fusarium, Gibberella and Cylindrocarpon through the screening in a variety of microorganisms. Fusarium oxysporum AKU 3702 showed high productivity of the enzyme and the cells containing the enzyme could be used repeatedly for this hydrolysis reaction. On incubation with the mycelia of this fungus, which had been cultivated in 3% glycerol, 0.5% Polypepton, 0.5% yeast extract and 0.5% corn steep liquor, pH 6.0,46.0% of the recemic pantoyl lactone (700 mg/ml) was hydrolyzed and the optical purity of the pantoic acid formed was 96% enantiomeric excess for the D-isomer.

真菌乳糖水解酶催化D-Pantoyl Lactone的分子内酯键的立体特异性水解，可用于光学分辨率分辨出乳腺Pantoyl Lactone。通过在多种微生物中筛选的几种属于镰刀菌，gibberella和cylindrocarpon的丝状真菌中发现了这种立体特异性水解反应的高活性。 Aku 3702的镰刀菌Aku fusarium aku显示出较高的酶的生产率，并且含有该酶的细胞可反复用于这种水解反应。 On incubation with the mycelia of this fungus, which had been cultivated in 3% glycerol, 0.5% Polypepton, 0.5% yeast extract and 0.5% corn steep liquor, pH 6.0,46.0% of the recemic pantoyl lactone (700 mg/ml) was hydrolyzed and the optical purity of the pantoic acid formed was 96% enantiomeric excess for the D-isomer.

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