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Structure and function of a novel fungal enzyme, lactonohydrolase, and its application to optical resolution of pantolactone

Structure and function of a novel fungal enzyme, lactonohydrolase, and its application to optical resolution of pantolactone
新型真菌酶内酯水解酶的结构和功能及其在泛内酯光学拆分中的应用
批准号:
06453167
负责人:
SHIMIZU Sakayu
金额:
$5.06万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995

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中文摘要
翻译
真菌内酯水解酶可催化d -泛酰基内酯分子内酯键的立体特异性水解,可用于外消旋泛酰基内酯的光学拆分。通过对多种微生物的筛选,发现该立体特异性水解反应在镰刀菌属、赤霉素属和圆柱碳属的几种丝状真菌中均具有高活性。尖孢镰刀菌AKU 3702具有较高的酶产率,含有该酶的细胞可重复用于该酶的水解反应。在3%甘油、0.5%多肽、0.5%酵母提取物和0.5%玉米浸泡液中培养的真菌菌丝体,在pH 6.0条件下水解46.0% (700 mg/ml)的泛酸,形成的泛酸光学纯度为96%以上的对映异构体。
英文摘要
A fungal lactonohydrolase catalyzes the stereospecific hydrolysis of the intramolecular ester bond of D-pantoyl lactone and is useful for optical resolution of racemic pantoyl lactone. High activity of this stereospecific hydrolysis reaction was found in several filamentous fungi belonging to the genera Fusarium, Gibberella and Cylindrocarpon through the screening in a variety of microorganisms. Fusarium oxysporum AKU 3702 showed high productivity of the enzyme and the cells containing the enzyme could be used repeatedly for this hydrolysis reaction. On incubation with the mycelia of this fungus, which had been cultivated in 3% glycerol, 0.5% Polypepton, 0.5% yeast extract and 0.5% corn steep liquor, pH 6.0,46.0% of the recemic pantoyl lactone (700 mg/ml) was hydrolyzed and the optical purity of the pantoic acid formed was 96% enantiomeric excess for the D-isomer.
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DOI: --
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作者: []
通讯作者:
Kataoka, M.et al.: "Lactonohydrolase catalyzed optical resolution of pantoyl lactone" Appl.Microbiol.Biotechnol.44. 333-338 (1995)
Kataoka, M.et al.:“内酯水解酶催化泛酰内酯的光学拆分”Appl.Microbiol.Biotechnol.44。
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通讯作者:
Shirasaka, N. et al.: "Occurrence of a furan fatty acid in marine bacteria" Biochim. Biophys. Acta. 1258. 225-227 (1995)
Shirasaka, N. 等人:“海洋细菌中呋喃脂肪酸的出现”Biochim。
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通讯作者:
清水 昌: "「スクリーニング」にまつわるあいまいな話" バイオサイエンスとインダストリー. 52. 134 (1994)
Masaru Shimizu:“关于‘筛选’的模糊故事”《生物科学与工业》52. 134 (1994)。
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共 16 条
    Production of novel functional lipids by manifold application of microbial capacity
    • 批准号:
      18208009
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $30.53万
    • 财政年份:
      2006
    • 负责人:
      SHIMIZU Sakayu
    • 依托单位:
    Novel multi-purpose bioreduction system for the large-scale production of chiral alcohols
    • 批准号:
      13853009
    • 项目类别:
      Grant-in-Aid for Scientific Research (S)
    • 资助金额:
      $78.96万
    • 财政年份:
      2001
    • 负责人:
      SHIMIZU Sakayu
    • 依托单位:
    Development of enzymatic resolution process of pantolactone by fungal lactonase
    • 批准号:
      08556013
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $6.4万
    • 财政年份:
      1996
    • 负责人:
      SHIMIZU Sakayu
    • 依托单位:
    Screening, functional analysis and application of polyunsaturated fatty acid-desaturase inhibitors
    • 批准号:
      08456050
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $4.54万
    • 财政年份:
      1996
    • 负责人:
      SHIMIZU Sakayu
    • 依托单位:
    海外基金