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Molecular Mechanism of Cytoplasmic Male Sterility in Sugarbeet (Beta vulgaris L.)

甜菜（Beta vulgaris L.）细胞质雄性不育的分子机制

基本信息

批准号：
06454039
负责人：
MIKAMI Tetsuo
金额：
$ 3.97万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454039/
关键词：
sugarbeet cytoplasmic male sterility mitochondria coxI Rf transcription flower bud RNA Northern blot analysis 構造変異 rps13 rps3 orf171 orfB

项目摘要

To study the molecular basis of cytoplasmic male sterility (CMS) in sugarbeet, we have conducted a detailed comparison of the mitochondrial genomes of two sugarbeet lines, one containing a fertile (N) cytoplasm and the other a male sterile one. The two genomes have approximately 330kb of common sequences but there is considerable variation in sequence organization which can be accounted for by genome rearrangements.Transcripts for known mitochondrial genes and regions of the two sugarbeet genomes known to be rearranged relative to one another were compared among male-fertile, CMS and nuclear-restored sugarbeet genotypes. In most instances, identical transcript patterns were observed for all three, mtRNAs (prepared from leaves or flower buds), indicating that rearrangement events did not occur within transcriptional units. Altered transcript patterns were, however, identified for six genes, coxI,coxII,atpA,rps3, orf324, and orf1.Interestingly, the coxI transcripts were found to be significantly affected by nuclear background, whereas unclear background has no effect on the transcripts of other five genes.The results suggest that nuclear-restorer gene (s) have an effect on coxI transcript patterns and implicate coxI in sugarbeet male sterility.

为了研究甜菜细胞质雄性不育（CMS）的分子基础，我们对两个甜菜品系的线粒体基因组进行了详细的比较，其中一个含有可育（N）细胞质，另一个含有雄性不育细胞质。这两个基因组有大约330 kb的共同序列，但有相当大的变化，序列组织，可以占基因组rearrangements.Transcripts已知的线粒体基因和区域的两个甜菜基因组已知的相对于彼此重排之间进行了比较雄性可育，CMS和核恢复甜菜基因型。在大多数情况下，观察到相同的转录模式，所有三个，线粒体RNA（从叶或花蕾制备），表明重排事件没有发生在转录单位。coxI、coxII、atpA、rps3、orf324和orf1等6个基因的转录模式发生了改变，其中coxI基因的转录受核背景的影响显著，而核背景不清对其他5个基因的转录没有影响，说明核恢复基因对coxI基因的转录模式有影响，coxI基因可能与甜菜雄性不育有关。