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Identification of mitochondrial male sterility gene and molecular dissection of pollen abortion sugarbeet

甜菜花粉败育线粒体雄性不育基因鉴定及分子解剖

基本信息

批准号：
13306001
负责人：
MIKAMI Tetsuo
金额：
$ 31.95万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13306001/
关键词：
sugarbeet cytoplasmic male sterility mitochondrial genes mechanism of pollen abortion ORF preSatp6 35-kDa protein mitochondrial genome preSATP6 in organello翻訳ウエスタン解析キメラ遺伝子稔性回復核遺伝子ノーザン解析

项目摘要

We determined the complete nucleotide sequence of the mtDNA from cytoplasmic male sterile sugarbeet (cv.TK81-MS), with the expectation that the data will aid in identifying genes responsible for male sterility. The entire genome comprises 501020 bp. Although the mitochondrial genome of TK81-MS is 130kb larger than the normal fertile mtDNA (TK81-O), there is no difference in the content of identified genes between the two mitochondrial genomes. Most of the additional sequences in TK81-MS mtDNA are composed of sequence duplications and increased intergenic regions of unknown origin. In an attempt to identify the TK81-MS-specific ORF(open reading frame) involved in male sterility, we found that atp6 gene from TK81-MS has a 387-codon presequence which shows no similarity to the presequence of either TK81-O atp6, or any other sequences of TK81-O mtDNA. The TK81-MS atp6 presequence is actually transcribed and translated in the mitochondrion, resulting in the expression of a 35-kDa protein. The variant 35-kDa protein was revealed to be characteristic of the male-sterile mitochondria and to accumulate in flower buds. This study is the first to describe that the atp6 presequence is actually translated in mitochondria and causally related with the male sterility phenotype.

我们测定了甜菜细胞质雄性不育系(cv.TK81-MS)mtDNA的完整核苷酸序列，以期这些数据将有助于识别与雄性不育有关的基因。整个基因组由501020个核苷酸组成。尽管TK81-MS的线粒体基因组比正常可育mtDNA(TK81-O)大130kb，但两种线粒体基因组中鉴定基因的含量没有差异。TK81-MS线粒体DNA的大部分附加序列由序列重复和来源不明的基因间隔区组成。为了鉴定TK81-MS特异的ORF(开放阅读框)与男性不育有关，我们发现TK81-MS的atp6基因有一个387密码子的前序列，与TK81-O的前序列或TK81-O线粒体DNA的任何其他序列都没有相似性。TK81-MS atp6前序列实际上是在线粒体中转录和翻译的，导致了35 kDa蛋白的表达。变异的35 kDa蛋白被发现是雄性不育线粒体的特征，并在花蕾中积累。这项研究首次描述了atp6前序列实际上是在线粒体中翻译的，并与男性不育表型存在因果关系。